Assessment of plasma nitric oxide concentration and erythrocyte arginase activity in dairy cows with traumatic reticuloperitonitis

The aim of this study was to evaluate plasma nitric oxide (NO) concentrations, erythrocyte arginase (ARG) activity, plasma fibrinogen (Fb) and serum iron (Fe) levels and some biochemical parameters in dairy cows with traumatic reticuloperitonitis (TRP). The animal material of the study consisted of 14 Swiss Brown cows diagnosed with TRP (TRP group) between 4-8 years old brought to Firat University Animal Hospital Clinics and 14 healthy Swiss Brown cows (control group) aged 4-8 years obtained from dairy farms in different regions. Blood samples were taken from the vena jugularis of the animals. Concentrations of plasma NO, Fb, erythrocyte ARG activity, and some biochemical markers were determined after the serum and plasma of the receiving blood were separated. While the NO (318.9±5.8 vs. 270.3±9.6 μmol/L) concentrations of the TRP group were found to be significantly higher than the control group (P<0.001), the erythrocyte ARG activity (29.5±0.5 vs. 35.2±1.0 U/hb) was found to be higher in the control group (P<0.001). It was also observed that total protein (TP) (6.6±0.5 vs. 7.8±0.1 g/dL) (P<0.05) and Fb (914.3±68.6 vs. 265.4±19.8 mg/dL) (P<0.001) concentrations were higher in the TRP group, compared to the control group, while albumin (ALB) (1.9±0.2 vs. 3.1±0.1 g/dL) and Fe (47.00±5.29 vs.106.79±9.44 μg/dL) concentrations were significantly lower than the control group (P<0.001). In addition, a positive correlation was found between NO and Fb concentrations and between erythrocyte ARG activity and Fe concentrations. As a result, it was determined that NO concentrations were increased and erythrocyte ARG activity was not significant in dairy cows with TRP. In addition, increased plasma Fb concentration and decreased serum Fe concentration were determined in dairy cows with TRP. This study demonstrated that plasma NO, Fb and serum Fe concentrations in dairy cows with TRP may be useful markers for prognosis.

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Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows

Veterinary Research Communications ◽

10.1007/s11259-010-9355-8 ◽

2010 ◽

Vol 34 (4) ◽

pp. 315-321 ◽

Cited By ~ 21

Author(s):

DeJun Li ◽

YunFeng Liu ◽

YanFei Li ◽

Ying Lv ◽

XiaoYing Pei ◽

...

Keyword(s):

Nitric Oxide ◽

Dairy Cows ◽

Oxide Concentration ◽

Nitric Oxide Concentration

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Evaluation of serum homocysteine and nitric oxide concentrations compared with other biochemical parameters in sheep naturally infected with Fasciola hepatica

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.23634 ◽

2020 ◽

Vol 71 (2) ◽

pp. 2107

Author(s):

V. DENIZHAN ◽

S. KOZAT

Keyword(s):

Nitric Oxide ◽

Statistical Analysis ◽

Vitamin B12 ◽

Biochemical Parameters ◽

Fasciola Hepatica ◽

Control Group ◽

Serum Homocysteine ◽

Healthy Sheep ◽

Animal Material ◽

Folate And Vitamin B12

This study aims to determine the changes in serum homocysteine (Hcy) and nitric oxide (NO) concentrations in sheep naturally infected with F. hepatica. The animal material of the study consisted of a total of 50 sheep: 40 sheep with fascioliasis and 10 healthy sheep.The statistical analysis indicated that serum homocysteine concentrations, folate and vitamin B12 levels of the sheep infected with F. hepatica were higher than those of the control group (P<0.001 P<0.001 and P<0.05, respectively), whereas the nitric oxide levels of the sheep infected with F. hepatica were significantly lower than those of healthy sheep (P<0.001). In conclusion, it is thought that vitamin B12 and folate are not used sufficiently for the conversion of homocysteine to methionine in the remethylation cycle due to the damage in the liver tissue of sheep naturally infected with F. hepatica. This results in the increase of homocysteine which in turn inhibits the formation of nitric oxide.

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The impact of subchronic cadmium poisoning on the vascular effect of nitric oxide in rats

Human & Experimental Toxicology ◽

10.1191/0960327105ht536oa ◽

2005 ◽

Vol 24 (7) ◽

pp. 353-361 ◽

Cited By ~ 25

Author(s):

A Skoczynska ◽

H Martynowicz

Keyword(s):

Nitric Oxide ◽

Vascular Reactivity ◽

Perfusion Pressure ◽

Control Group ◽

Vascular Effect ◽

Cadmium Poisoning ◽

Oxide Concentration ◽

Nitric Oxide Concentration ◽

The Right ◽

The Impact

The aim of this study was to evaluate the impact of poisoning with cadmium in hypertensive doses (50 or 200 ppm in drinking water for three months) on the basal and stimulated release NO effect in the isolated and perfused rat mesenteric bed. Mesenteric artery preparation preconstricted by norepinephrine (0.5 μg/mL) was used to determine changes in its vascular resistance induced by e-NOS synthase blocker, N-ω-nitro-L-arginine (L-NOARG) injected in increasing doses from 1.0 to 200.0 μg or acetylcholine (ACh) administered in doses from 0.05 × 10-10 to 5.0 × 10-10 mol before and during L-NOARG infusion (1.0 μg/mL). Vascular reactivity was measured as an increase or decrease in perfusion pressure in the constant flow system. Rats poisoned with 50 or 200 ppm of cadmium demonstrated a significant decrease (P < 0.05) in vascular response to L-NOARG used in doses of 50 or 100 μg. The dose-response curve obtained for L-NOARG was shifted to the right and ED50 value was greater in the group of rats given cadmium in a dose of 200 ppm than in the controls (70.39 ± 10.7 versus 25.79 ± 4.8 μg, P < 0.01). These rats reacted with lower expressed vasodilatation to ACh in doses to 0.2 ± 10-10 mol. In all poisoned rats, L-NOARG enhanced the effect of ACh used in doses from 0.05 to 0.5 ± 10-10 mol, whereas in the control group this effect was only achieved at 0.1 ± 10-10 mol. The serum nitric oxide concentration was decreased (P B < 0.05) in both groups of cadmium-treated rats. These results suggest that cadmium in hypertensive doses modifies the vascular effect of NO in basal conditions and after stimulation by ACh.

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Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

Current Bioactive Compounds ◽

10.2174/1573407216999201224150602 ◽

2020 ◽

Vol 16 ◽

Author(s):

Maibam Beebina Chanu ◽

Biseshwori Thongam ◽

Khumukcham Nongalleima ◽

Hans Raj Bhat ◽

Surajit Kumar Ghosh ◽

...

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Biochemical Parameters ◽

Methanolic Extract ◽

Reducing Power ◽

Quercus Serrata ◽

Control Group ◽

Blood Cell Count ◽

Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

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