scholarly journals Evaluation of serum homocysteine and nitric oxide concentrations compared with other biochemical parameters in sheep naturally infected with Fasciola hepatica

2020 ◽  
Vol 71 (2) ◽  
pp. 2107
Author(s):  
V. DENIZHAN ◽  
S. KOZAT
Keyword(s):  
Nitric Oxide ◽  
Statistical Analysis ◽  
Vitamin B12 ◽  
Biochemical Parameters ◽  
Fasciola Hepatica ◽  
Control Group ◽  
Serum Homocysteine ◽  
Healthy Sheep ◽  
Animal Material ◽  
Folate And Vitamin B12

This study aims to determine the changes in serum homocysteine (Hcy) and nitric oxide (NO) concentrations in sheep naturally infected with F. hepatica. The animal material of the study consisted of a total of 50 sheep: 40 sheep with fascioliasis and 10 healthy sheep.The statistical analysis indicated that serum homocysteine concentrations, folate and vitamin B12 levels of the sheep infected with F. hepatica were higher than those of the control group (P<0.001 P<0.001 and P<0.05, respectively), whereas the nitric oxide levels of the sheep infected with F. hepatica were significantly lower than those of healthy sheep (P<0.001). In conclusion, it is thought that vitamin B12 and folate are not used sufficiently for the conversion of homocysteine to methionine in the remethylation cycle due to the damage in the liver tissue of sheep naturally infected with F. hepatica. This results in the increase of homocysteine which in turn inhibits the formation of nitric oxide.

scholarly journals Clinical influence of maternal serum homocysteine, folate and vitamin B12 in the development of pre-eclampsia

Pteridines ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Hongyan Zhao ◽  
Xu Zeng
Keyword(s):  
Vitamin B12 ◽  
Gestational Hypertension ◽  
Severe Disease ◽  
Serum Levels ◽  
Maternal Serum ◽  
Control Group ◽  
Mild Disease ◽  
Serum Homocysteine ◽  
Folate And Vitamin B12 ◽  
Serological Biomarkers

Abstract Background: The aim of this study was to investigate the correlation between maternal serum homocysteine (Hcy), folate, vitamin B12 (VitB12) and the development of pre-eclampsia (PE). Methods: Seventy-eight normal pregnant women (without hypertension and proteinuria during their pregnancy (control group)), 66 cases of gestational hypertension (GH group) and 82 cases of pre-eclampsia (PE group, with 56 cases of mild disease and 26 cases of severe disease) were include in this study. The maternal serum Hcy, folate and VitB12 level of the included cases were examined between 11 to 13 weeks gestation and compared between each group. Results: The serum levels of VitB12 were significantly different between the control, GH and PE groups (p<0.05). The serum levels of Hcy in the PE group were significantly higher than those of the control group (p<0.05). However, the serum levels of folate in the PE group were significantly lower than those of control group (p<0.05). Significant statistical differences in the maternal serum Hcy and folate were found between mild and severe PE patient groups (p<0.05). A significant correlation was found between maternal serum Hcy and VitB12 (r=-0.34, p=0.001). Conclusion: Hcy, folate and VitB12 may play an important role in the development of PE and could be potential serological biomarkers for early PE diagnosis.

scholarly journals Assessment of plasma nitric oxide concentration and erythrocyte arginase activity in dairy cows with traumatic reticuloperitonitis

2020 ◽  
Vol 70 (4) ◽  
pp. 1833
Author(s):  
A. KIRBAS ◽  
E. BAYDAR ◽  
F,M, KANDEMIR
Keyword(s):  
Nitric Oxide ◽  
Dairy Cows ◽  
Biochemical Markers ◽  
Biochemical Parameters ◽  
Serum Iron ◽  
Control Group ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
Animal Material ◽  
Traumatic Reticuloperitonitis

The aim of this study was to evaluate plasma nitric oxide (NO) concentrations, erythrocyte arginase (ARG) activity, plasma fibrinogen (Fb) and serum iron (Fe) levels and some biochemical parameters in dairy cows with traumatic reticuloperitonitis (TRP). The animal material of the study consisted of 14 Swiss Brown cows diagnosed with TRP (TRP group) between 4-8 years old brought to Firat University Animal Hospital Clinics and 14 healthy Swiss Brown cows (control group) aged 4-8 years obtained from dairy farms in different regions. Blood samples were taken from the vena jugularis of the animals. Concentrations of plasma NO, Fb, erythrocyte ARG activity, and some biochemical markers were determined after the serum and plasma of the receiving blood were separated. While the NO (318.9±5.8 vs. 270.3±9.6 μmol/L) concentrations of the TRP group were found to be significantly higher than the control group (P<0.001), the erythrocyte ARG activity (29.5±0.5 vs. 35.2±1.0 U/hb) was found to be higher in the control group (P<0.001). It was also observed that total protein (TP) (6.6±0.5 vs. 7.8±0.1 g/dL) (P<0.05) and Fb (914.3±68.6 vs. 265.4±19.8 mg/dL) (P<0.001) concentrations were higher in the TRP group, compared to the control group, while albumin (ALB) (1.9±0.2 vs. 3.1±0.1 g/dL) and Fe (47.00±5.29 vs.106.79±9.44 μg/dL) concentrations were significantly lower than the control group (P<0.001). In addition, a positive correlation was found between NO and Fb concentrations and between erythrocyte ARG activity and Fe concentrations. As a result, it was determined that NO concentrations were increased and erythrocyte ARG activity was not significant in dairy cows with TRP. In addition, increased plasma Fb concentration and decreased serum Fe concentration were determined in dairy cows with TRP. This study demonstrated that plasma NO, Fb and serum Fe concentrations in dairy cows with TRP may be useful markers for prognosis.

Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

2020 ◽  
Vol 16 ◽  
Author(s):  
Maibam Beebina Chanu ◽  
Biseshwori Thongam ◽  
Khumukcham Nongalleima ◽  
Hans Raj Bhat ◽  
Surajit Kumar Ghosh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Quercus Serrata ◽  
Control Group ◽  
Blood Cell Count ◽  
Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

scholarly journals Determination of selected hormones and biochemical parameters in Turkish Van cats with tail gland hyperplasia

2020 ◽  
Vol 76 (01) ◽  
pp. 6335-2020
Author(s):  
CUMALİ OZKAN ◽  
ABDULLAH KAYAR ◽  
ABDULLAH KAYA ◽  
SERKAN YİLDİRİM ◽  
ONUR ISKEFLİ ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Statistical Significance ◽  
Control Group ◽  
Hormone Levels ◽  
Testosterone Levels ◽  
Routine Clinical Examination ◽  
Animal Material ◽  
Tail Gland ◽  
The Difference

The present study was performed on Turkish Van cats with tail gland hyperplasia in order to determine changes in some hormone levels and biochemical parameters. Animal material consisted of 18 male Van cats of between 2-8 years of age, 12 with tail gland hyperplasia and 6 unaffected cats as the control group. Following routine clinical examination, hormone levels and biochemical parameters were measured by obtaining blood samples, and biopsy samples were obtained from the region and examined under a light microscope. When compared with the control group, testosterone levels were high although the difference between groups was not statistically significant. Similarly, no statistical significance was found between the groups in terms of other hormones. Among the hormonal parameters, the only remarkable increase was seen in the testosterone level. Among biochemical parameters, the alkaline phosphatase (ALP), aspartate amino transferase (AST), cholesterol and triglyceride levels were statistically different, while other biochemical parameters were not statistically significant. While AST and cholesterol results were increased, ALP and triglyceride levels were decreased. As a result, we conclude that the disease is an important problem which occurs extensively in mature male Van cats, particularly during and after mating periods. We also conclude that the disease might be associated with an increase in testosterone levels. Presenting this study is beneficial as there are limited reports about this disease in cats. This is the first study presenting tail gland hyperplasia with some hormonal levels and biochemical parameters in male Van cats.

An Analysis of Transcobalamin II Gene Polymorphisms and Serum Levels of Homocysteine, Folate and Vitamin B12 in Chinese Patients with Crohn's Disease

2017 ◽  
Vol 35 (5) ◽  
pp. 463-471
Author(s):  
Shuzi Zheng ◽  
Chaoqun Wu ◽  
Wei Yang ◽  
Xuanping Xia ◽  
Xiuqing Lin ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Vitamin B12 ◽  
Mutant Allele ◽  
Vitamin B12 Deficiency ◽  
Folate Deficiency ◽  
Serum Homocysteine ◽  
B12 Deficiency ◽  
Folate And Vitamin B12 ◽  
Transcobalamin Ii

Objectives: The study aimed to investigate the association of Crohn's disease (CD) with transcobalamin II (TCN2) polymorphisms and serum homocysteine, folate, and vitamin B12 levels. Methods:TCN2 (rs1801198, rs9606756) were genotyped by iMLDR in 389 CD patients and 746 controls. Furthermore, 102 CD patients and 153 controls were randomly selected for examination of serum homocysteine, folate, and vitamin B12 levels by enzymatic cycling assay and chemiluminescence immunoassay, respectively. Results: Mutant allele (G) and genotype (AG + GG) of (rs9606756) were higher in CD patients than in controls (both p < 0.05). So were they in ileocolonic CD patients and stricturing CD patients compared to controls (all p < 0.05). Mutant allele (G) and genotype (CG + GG) of (rs1801198) were more prevalent in stricturing CD patients than in controls (both p < 0.05). Compared to controls, average homocysteine level was enhanced in CD patients (p = 0.003), whereas average folate and vitamin B12 levels were reduced in CD patients (both p < 0.001). The prevalence of hyperhomocysteinemia, folate deficiency, and vitamin B12 deficiency was higher in CD patients than in controls (all p < 0.01). Both folate deficiency and vitamin B12 deficiency were independently related to risk of CD (both p < 0.01). Conclusion:TCN2 (rs1801198, rs9606756) polymorphisms as well as folate deficiency and vitamin B12 deficiency are correlated with CD.

scholarly journals Hyperhomocysteinemia and Low Folate and Vitamin B12 Are Associated with Vascular Dysfunction and Impaired Nitric Oxide Sensitivity in Morbidly Obese Patients

Nutrients ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 2014 ◽  
Author(s):  
Mohamed Haloul ◽  
Smita Jagdish Vinjamuri ◽  
Dina Naquiallah ◽  
Mohammed Imaduddin Mirza ◽  
Maryam Qureshi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Adipose Tissue ◽  
Vitamin B12 ◽  
Ex Vivo ◽  
Vascular Dysfunction ◽  
Morbidly Obese ◽  
No Production ◽  
Obese Subjects ◽  
Synthase Inhibitor ◽  
Folate And Vitamin B12

There is a high prevalence of hyperhomocysteinemia that has been linked to high cardiovascular risk in obese individuals and could be attributed to poor nutritional status of folate and vitamin B12. We sought to examine the association between blood homocysteine (Hcy) folate, and vitamin B12 levels and vascular dysfunction in morbidly obese adults using novel ex vivo flow-induced dilation (FID) measurements of isolated adipose tissue arterioles. Brachial artery flow-mediated dilation (FMD) was also measured. Subcutaneous and visceral adipose tissue biopsies were obtained from morbidly obese individuals and non-obese controls. Resistance arterioles were isolated in which FID, acetylcholine-induced dilation (AChID), and nitric oxide (NO) production were measured in the absence or presence of the NO synthase inhibitor, L-NAME, Hcy, or the superoxide dismutase mimetic, TEMPOL. Our results demonstrated that plasma Hcy concentrations were significantly higher, while folate, vitamin B12, and NO were significantly lower in obese subjects compared to controls. Hcy concentrations correlated positively with BMI, fat %, and insulin levels but not with folate or vitamin B12. Brachial and arteriolar vasodilation were lower in obese subjects, positively correlated with folate and vitamin B12, and inversely correlated with Hcy. Arteriolar NO measurements and sensitivity to L-NAME were lower in obese subjects compared to controls. Finally, Hcy incubation reduced arteriolar FID and NO sensitivity, an effect that was abolished by TEMPOL. In conclusion, these data suggest that high concentrations of plasma Hcy and low concentrations of folate and vitamin B12 could be independent predictors of vascular dysfunction in morbidly obese individuals.

Total serum homocysteine, folate and vitamin B12 in a Greek school age population

2006 ◽  
Vol 25 (5) ◽  
pp. 797-802 ◽  
Author(s):  
Dimitrios Papandreou ◽  
Ioannis Mavromichalis ◽  
Areti Makedou ◽  
Israel Rousso ◽  
Malamatenia Arvanitidou
Keyword(s):  
Vitamin B12 ◽  
Total Serum ◽  
School Age ◽  
Serum Homocysteine ◽  
Folate And Vitamin B12
Sign in / Sign up

Export Citation Format

Share Document