scholarly journals Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1839
Author(s):  
Harlinda Kuspradini ◽  
Indah Wulandari ◽  
Agmi Sinta Putri ◽  
Sabeti Yulis Tiya ◽  
Irawan Wijaya Kusuma
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
Free Radical Formation ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent ◽  
Microdilution Broth

Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.

scholarly journals Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1839 ◽  
Author(s):  
Harlinda Kuspradini ◽  
Indah Wulandari ◽  
Agmi Sinta Putri ◽  
Sabeti Yulis Tiya ◽  
Irawan Wijaya Kusuma
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
Free Radical Formation ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent ◽  
Microdilution Broth

Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

2017 ◽  
pp. 59-63
Author(s):  
Abdul Sadat ◽  
Mayukh Hore ◽  
Kaushik Chakraborty ◽  
Subhrajyoti Roy
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

scholarly journals Phytochemical Constituent and Antioxidant Activity of Thaumatococcus daniellii Benn (Benth.) Leaves (Food Wrapper)

2016 ◽  
Vol 2 ◽  
pp. 55-61
Author(s):  
Oke Ibukunoluwa Ayodeji ◽  
Olufemi Adeleye ◽  
Olugbenga Dada ◽  
Olaoluwa Adeyemi ◽  
Godswill N. Anyasor
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Ferric Ion ◽  
Aqueous Fraction ◽  
Standard Procedures ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent

Thaumatoccocus daniellii Benn (Benth.) is an organic food wrapper that influences the color and flavour of most foods processed and packaged with it. Hence, this study was designed to evaluate the phytochemical constituent and antioxidant activity of T. daniellii leave. Quantitative phytochemical analysis was carried out using standard procedures while antioxidant activity was assayed using 2’,2’diphenylpicrylhydrazine (DPPH) and ferric ion reducing antioxidant potential (FRAP). Results showed that T. daniellii leaves contained flavonoids, polyphenols, alkaloids and saponins. Furthermore, aqueous leaf fraction had higher quantity of polyphenols (0.41 ± 0.1 mg% gallic acid) and flavonoids (0.28 ± 0.1 mg% quercetin) than polyphenols (0.23 ± 0.3 mg% gallic acid) and flavonoids (0.11 ± 0.1 mg% quercetin) in hexane leaf fraction. Investigation of antioxidant activity revealed that 100 - 500 mg/ml aqueous leaf fraction exhibited a significantly (P<0.05) higher DPPH radical scavenging activity than the hexane leaf fraction. In addition, the aqueous fraction of T. daniellii leaves exhibited a significantly (P<0.05) higher ferric ion reducing potential than hexane leaf fraction. Thus, data from this study indicated that T. daniellii leaves color and flavour enhancing properties could be attributed to the presence of polyphenols and flavonoids. In addition, its use as food wrapper may introduce phyto-antioxidants into foods processed and packaged with T. daniellii leaves.

scholarly journals GC-MS profiling and DPPH radical scavenging activity of the bark of Tampoi (Baccaurea macrocarpa)

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1977
Author(s):  
Erwin Erwin ◽  
Widar Ristiyani Pusparohmana ◽  
Indah Permata Sari ◽  
Rita Hairani ◽  
Usman Usman
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
East Kalimantan ◽  
Dpph Radical Scavenging

Background : Tampoi ( Baccaurea macrocarpa) is a tropical rainforest plant that produces edible fruit and is native to Southeast Asia, especially East Kalimantan, Indonesia. Previous research showed that Tampoi potentially can be developed as a drug. It was reported that the extract of Tampoi fruit displayed antioxidant activity, which was correlated with its phenolic and flavonoid substances. There is no information about the antioxidant activity of other parts of this plant, such as the bark, which might also have this kind of activity. Therefore, the aim of this study was to evaluate the phytochemical using GC-MS analysis, toxicity againt Artemia salina, and antioxidant activity with DPPH radical scavenging method of the bark of Tampoi. Methods : The bark of Tampoi was extracted with methanol and concentrated using rotary evaporator to obtain the methanol extract of the bark. Secondary metabolites of this extract was determined using phytochemical analysis. Afterward, the methanol extract was tested for its toxicity using brine shrimp lethality test and antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method. Results : Phytochemical evaluation results showed that the methanol extract of bark of this plant contains several secondary metabolites including alkaloids, flavonoids, phenolics, steroids, and triterpenoids. The toxicity test displayed no toxic property due to a LC 50 value above 1000 ppm. For antioxidant activity, the result exhibited that the methanol extract of bark of this plant could be categorized as an active extract with IC 50 value of 11.15 ppm. Moreover, based on gas chromatography-mass spectrometer analysis, there are 37 isolated compounds from the bark, one of which is methylparaben, a phenolic predicted to act as an antioxidant. Conclusion: The results obtained in this research demonstrated that the bark of Tampoi (B. macrocarpa) has potential as an antioxidant.

Chemical composition, antioxidant activity and antimicrobial activity of essential oil from Citrus aurantium L zest against some pathogenic microorganisms

2019 ◽  
Vol 74 (5-6) ◽  
pp. 105-111 ◽  
Author(s):  
Desislava Teneva ◽  
Rositsa Denkova-Kostova ◽  
Bogdan Goranov ◽  
Yana Hristova-Ivanova ◽  
Aleksandar Slavchev ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Citrus Aurantium ◽  
Dpph Radical ◽  
Chromatography Analysis ◽  
Dpph Radical Scavenging

Abstract This study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of Citrus aurantium L zest essential oil. The identification of the chemical compounds was done using chromatography analysis. The antioxidant activity was studied by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Results showed that the main components of the essential oil were limonene (85.22%), β-myrcene (4.3%), and α-pinene (1.29%). Regarding the DPPH radical scavenging ability, the zest essential oil showed higher activity than limonene. The antimicrobial activity of the essential oil against pathogenic [Staphylococcus aureus NBIMCC 3703, Salmonella sp. (clinical isolate), Pseudomonas aeruginosa NBIMCC 1390, Bacillus subtilis NBIMCC 1208, Escherichia coli NBIMCC 3702] microorganisms by disc-diffusion method was examined. Gram-positive bacteria were more sensitive to the oil (inhibition zones being between 9 and 12.5 mm) and the minimum inhibitory concentration was more than 600 ppm; Gram-negative bacteria were less sensitive. The obtained essential oil displayed promising results for its application as a biopreservative agent.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

DPPH Radical Scavenging Activity of Kaempferol

2013 ◽  
Vol 781-784 ◽  
pp. 1294-1297 ◽  
Author(s):  
Ping Liu ◽  
Yun Tao Gao ◽  
Jiao Jiao Yu ◽  
Jia Wei Cha ◽  
Dong Mei Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

scholarly journals Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Pranabendu Mitra ◽  
Venkatesh Meda ◽  
Rick Green
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Vacuum Drying ◽  
Anthocyanin Content ◽  
Dpph Radical ◽  
Total Anthocyanin ◽  
Hot Air ◽  
Total Anthocyanin Content ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05.  DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P<0.05.

scholarly journals ANTIOXIDANT ACTIVITY OF METHYL GALLATE ISOLATED FROM THE LEAVES OF Toona sureni

2010 ◽  
Vol 9 (3) ◽  
pp. 457-460 ◽  
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methyl Gallate ◽  
Antioxidant Compound ◽  
Ic50 Value ◽  
Isolated Compound ◽  
Dpph Radical Scavenging

An antioxidant compound has been isolated from the leaves of Toona sureni (Blume) Merr. The structure was determined to be methyl 3,4,5-trihydroxybenzoate (methyl gallate), based on UV-vis, FTIR, NMR and MS spectra. The isolated compound exhibited potent antioxidant activity in the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging test, with IC50 value 1.02 μg/mL.   Keywords: antioxidant activity, methyl gallate, Toona sureni

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