Effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats

Background: Tooth movement induced by the application of orthodontic force is facilitated by bone remodelling cells and chemical mediators. Vitamin E has anti-inflammatory properties, which helps in suppressing the damaging effects of oxygen free radicals in cells during bone formation. This study aimed to evaluate the effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats. Methods: Wistar rats (n=56) were divided into two groups. Group 1 served as the control groups, while group 2 was given vitamin E for 14 days before application of orthodontic force. Each group was divided into four subgroups (n=7), corresponding to the number of days orthodontic force lasted, i.e. 0, 1, 3, 7 days. At each of these four time points, distance measurements and quantity of osteoblasts-osteoclasts were measured in each rat. Results: Tooth movement distance was increased for group 2 than group 1 for all time intervals, but this difference was only statistically different on day 3 (p=0.001). For both groups, tooth movement was significantly different between each time interval in each group (p=0.041). The mean number of osteoblast cells was increased for group 2 compared to group 1 for all time intervals (p<0.05), but was not significant different between time intervals (p=0.897). The number of osteoclasts was not significantly different between groups, but it was statistically different between time intervals (p=0.004). Conclusion: Present outcomes demonstrate that vitamin E contributes to faster tooth movement compared to control group. It also stimulates more bone formation without reducing the bone resorption.

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Effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats: a prelimary study

F1000Research ◽

10.12688/f1000research.25709.2 ◽

2020 ◽

Vol 9 ◽

pp. 1093

Author(s):

Erliera Sufarnap ◽

Darmayanti Siregar ◽

Yumi Lindawati

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Control Groups ◽

Osteoblast Cells ◽

Time Intervals ◽

Orthodontic Force ◽

Group 2 ◽

Group 1

Background: Tooth movement induced by the application of orthodontic force was initiated by inflammatory process. Studies have shown that vitamin E has an anti-inflammatory and antioxidant properties which perhaps could inhibit the tooth to move. This study aimed to evaluate the effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats. Methods: Wistar rats (n=56) were divided into two groups. Group 1 served as the control groups, while group 2 was given vitamin E for 14 days before application of orthodontic force. Each group was divided into four subgroups (n=7), corresponding to the number of days orthodontic force lasted, i.e. 0, 1, 3, 7 days. At each of these four time points, distance measurements and quantity of osteoblasts-osteoclasts were measured in each rat. Results: Tooth movement distance was increased for group 2 than group 1 for all time intervals, but this difference was only statistically different on day 3 (p=0.001). For both groups, tooth movement was significantly different between each time interval in each group (p=0.041). The mean number of osteoblast cells was increased for group 2 compared to group 1 for all time intervals (p<0.05), but was not significant different between time intervals (p=0.897). The number of osteoclasts was not significantly different between groups, but it was statistically different between time intervals (p=0.004). Conclusion: The outcome of this study demonstrated that group 2 resulted a better tooth movement compared to group 1 on day 3, based on the distance measurement. The osteoclast cell numbers were the same within control groups, whilst the number of osteoblast cells in group 2 was significantly higher than those in group 1.

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Effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats: a prelimary study

F1000Research ◽

10.12688/f1000research.25709.3 ◽

2020 ◽

Vol 9 ◽

pp. 1093

Author(s):

Erliera Sufarnap ◽

Darmayanti Siregar ◽

Yumi Lindawati

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Control Groups ◽

Osteoblast Cells ◽

Time Intervals ◽

Orthodontic Force ◽

Group 2 ◽

Group 1

Background: Tooth movement induced by the application of orthodontic force was initiated by inflammatory process. Studies have shown that vitamin E has an anti-inflammatory and antioxidant properties which perhaps could inhibit the tooth to move. This study aimed to evaluate the effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats. Methods: Wistar rats (n=56) were divided into two groups. Group 1 served as the control groups, while group 2 was given vitamin E for 14 days before application of orthodontic force. Each group was divided into four subgroups (n=7), corresponding to the number of days orthodontic force lasted, i.e. 0, 1, 3, 7 days. At each of these four time points, distance measurements and quantity of osteoblasts-osteoclasts were measured in each rat. Results: Tooth movement distance was increased for group 2 than group 1 for all time intervals, but this difference was only statistically different on day 3 (p=0.001). For both groups, tooth movement was significantly different between each time interval in each group (p=0.041). The mean number of osteoblast cells was increased for group 2 compared to group 1 for all time intervals (p<0.05), but was not significant different between time intervals (p=0.897). The number of osteoclasts was not significantly different between groups, but it was statistically different between time intervals (p=0.004). Conclusion: The outcome of this study demonstrated that group 2 resulted a better tooth movement compared to group 1 and significantly found on day 3, based on the distance measurement. The osteoclast cell numbers were the same within both control groups, whilst the number of osteoblast cells in group 2 was significantly higher than those in group 1.

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The role of inhibition of osteocyte apoptosis in mediating orthodontic tooth movement and periodontal remodeling: a pilot study

Progress in Orthodontics ◽

10.1186/s40510-021-00366-4 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Michele Kaplan ◽

Zana Kalajzic ◽

Thomas Choi ◽

Imad Maleeh ◽

Christopher L. Ricupero ◽

...

Keyword(s):

Bone Density ◽

Alveolar Bone ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Tartrate Resistant Acid Phosphatase ◽

Osteocyte Apoptosis ◽

Saline Administration ◽

Group 2 ◽

Group 1

Abstract Background Orthodontic tooth movement (OTM) has been shown to induce osteocyte apoptosis in alveolar bone shortly after force application. However, how osteocyte apoptosis affects orthodontic tooth movement is unknown. The goal of this study was to assess the effect of inhibition of osteocyte apoptosis on osteoclastogenesis, changes in the alveolar bone density, and the magnitude of OTM using a bisphosphonate analog (IG9402), a drug that affects osteocyte and osteoblast apoptosis but does not affect osteoclasts. Material and methods Two sets of experiments were performed. Experiment 1 was used to specifically evaluate the effect of IG9402 on osteocyte apoptosis in the alveolar bone during 24 h of OTM. For this experiment, twelve mice were divided into two groups: group 1, saline administration + OTM24-h (n=6), and group 2, IG9402 administration + OTM24-h (n=6). The contralateral unloaded sides served as the control. The goal of experiment 2 was to evaluate the role of osteocyte apoptosis on OTM magnitude and osteoclastogenesis 10 days after OTM. Twenty mice were divided into 4 groups: group 1, saline administration without OTM (n=5); group 2, IG9402 administration without OTM (n=5); group 3, saline + OTM10-day (n=6); and group 4, IG9402 + OTM10-day (n=4). For both experiments, tooth movement was achieved using Ultra Light (25g) Sentalloy Closed Coil Springs attached between the first maxillary molar and the central incisor. Linear measurements of tooth movement and alveolar bone density (BVF) were assessed by MicroCT analysis. Cell death (or apoptosis) was assessed by terminal dUTP nick-end labeling (TUNEL) assay, while osteoclast and macrophage formation were assessed by tartrate-resistant acid phosphatase (TRAP) staining and F4/80+ immunostaining. Results We found that IG9402 significantly blocked osteocyte apoptosis in alveolar bone (AB) at 24 h of OTM. At 10 days, IG9402 prevented OTM-induced loss of alveolar bone density and changed the morphology and quality of osteoclasts and macrophages, but did not significantly affect the amount of tooth movement. Conclusion Our study demonstrates that osteocyte apoptosis may play a significant role in osteoclast and macrophage formation during OTM, but does not seem to play a role in the magnitude of orthodontic tooth movement.

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Vitamin E supplementation modulates gingival crevicular fluid lipid peroxidation and antioxidant levels in patients with orthodontic tooth movement

Cell Biochemistry and Function ◽

10.1002/cbf.1833 ◽

2011 ◽

Vol 30 (5) ◽

pp. 376-381 ◽

Cited By ~ 4

Author(s):

Elçin Esenlik ◽

Mustafa Nazıroğlu ◽

Cahide Açıkalın ◽

Ishak Suat Övey

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

Crevicular Fluid ◽

Vitamin E Supplementation

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Preventing intraperitoneal adhesions with vitamin E and sodium hyaluronate/carboxymethylcellulose: a comparative study in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502008000100007 ◽

2008 ◽

Vol 23 (1) ◽

pp. 36-41 ◽

Cited By ~ 14

Author(s):

Fredy Corrales ◽

Marcelo Corrales ◽

Carlos Cauduro Schirmer

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Adhesion Formation ◽

Sodium Hyaluronate ◽

Postoperative Adhesions ◽

Intraperitoneal Adhesions ◽

Group 4 ◽

Group 3 ◽

Group 2 ◽

Group 1

PURPOSE: To compare the effectiveness of intraperitoneally administered vitamin E with the sodium hyaluronate/carboxymethylcellulose membrane (HA/CBMC) in preventing postoperative intraperitoneal adhesion formation. METHODS: Sixty Wistar rats underwent a laparotomy and adhesions were induced (IA). The animals were divided into four groups: group 1, control (IA); group 2 (IA + Vitamin E): group 3 (IA+HA/CBMC) and group 4 (IA+ Vitamin E + HA/CBMC). The Vitamin E (groups 2 and 4) and HA/CBMC (groups 3 and 4) were administered intraperitoneally before the abdominal wall was closed. After 30 days, adhesions were classified by an independent surgeon. RESULTS: Three animals died; one from group 3 and two from group 4. All control animals had substantial adhesions compared with unsubstantial adhesions observed in 11/15 in group 2 (P = 0.000), 11/14 in group 3 (P = 0.001), and 10/13 in group 4 (P = 0.000). CONCLUSION: Vitamin E, administered intraperitoneally, is as effective as HA/CBMC in preventing postoperative adhesions.

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Hypercaloric Diet and Vitamin E Intake

Materiale Plastice ◽

10.37358/mp.19.4.5287 ◽

2019 ◽

Vol 56 (4) ◽

pp. 937-941

Author(s):

Catalina Radulescu ◽

Daniela Miricescu ◽

Alexandra Totan ◽

Andreea Cristina Didilescu ◽

Iulia-Ioana Stanescu ◽

...

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Glycolic Acid ◽

Redox Status ◽

Plga Nanoparticles ◽

Antioxidant Effect ◽

Daily Dose ◽

Group 2 ◽

Hypercaloric Diet ◽

Group 1

PLGA (poly-lactic-co-glycolic acid) nanoparticles represent an important synthetic biocomponent that has the potential to be a promising carrier of drugs, proteins, nucleic acids, due to its biodegradability and minimal side effects. The aim of our study was to observe the antioxidant effect of vitamin E loaded in PLGA nanoparticles administered over a period of 3 weeks in Wistar rats treated with a hypercaloric diet. Glutathione (GSH) and malondiadehyde (MDA) biomarkers determined from liver lysate were analyzed to evaluate the oxidative stress (OS) induced by the hypercaloric diet. The results of our study revealed a statistically significant increase for GSH and vitamin E in group 2 of Wistar rats receiving hypercaloric diet and a daily dose of vitamin E versus group 1 (p[0.005). The antioxidant effect of vitamin E was also observed by the statistically significant decrease of MDA in group 2 of Wistar rats compared with group 1. The daily dose of vitamin E has improved the liver redox status of group 2 Wistar rats.

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Vitamin E supplementation reduces stress levels from orthodontic force in Wistar rats (Rattus norvegicus)

The Saudi Dental Journal ◽

10.1016/j.sdentj.2021.09.004 ◽

2021 ◽

Author(s):

Erliera Sufarnap ◽

Syafruddin Ilyas ◽

Ervina Sofyanti ◽

Darmayanti Siregar ◽

Yumi Lindawati ◽

...

Keyword(s):

Vitamin E ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Orthodontic Force ◽

Stress Levels ◽

Vitamin E Supplementation

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PROFIL OSTEOBLAS DAN OSTEOKLAS TULANG ALVEOLAR PADA MODEL TIKUS DIABETES TAHAP AWAL DENGAN APLIKASIGAYA ORTODONTI YANG BERBEDA

el–Hayah ◽

10.18860/elha.v5i2.3025 ◽

2015 ◽

Vol 5 (2) ◽

pp. 97

Author(s):

Nuzulul Hikmah

Keyword(s):

Bone Remodeling ◽

Wistar Rats ◽

Alveolar Bone ◽

Tooth Movement ◽

Early Stage ◽

Orthodontic Tooth Movement ◽

Orthodontic Force ◽

Force Application ◽

Alveolar Bone Remodeling ◽

Diabetic Treatment

Orthodontic tooth movement is obtained through alveolar bone remodeling. Alveolar bone remodeling includes resorption process that played by osteoclasts and bone formed process that played by osteoblasts. Diabetes affects on orthodontic tooth movement. The magnitude of orthodontic force that applied in the early stages of diabetic conditions, would be a consideration of the alveolar bone remodeling process. The purpose of this study was to determine osteoblast and osteoclast profile in early stage of rat diabetic models with different orthodontic force application. 24 Wistar rats were divided into three groups of controls andthree groups of early stage of diabetic treatment with different orthodontic force application (10, 20, and 30 gramforce/grf). The results showed an increased of osteoclast numbers in early stage of diabetes and will be increased along with the increased of orthodontic force. The results also showed a decreased of osteoblast number in early stage of diabetes, but it would be increased along with the increased of orthodontic force.

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Debonding Shear Strength of Orthodontic Tubes Bonded to Skeletal Anchorage Miniplates with Different Agents

The Open Dentistry Journal ◽

10.2174/1874210601913010551 ◽

2019 ◽

Vol 13 (1) ◽

pp. 551-556

Author(s):

Lucas Da Silva Meirelles ◽

Orion L. Haas ◽

Neimar Scolari ◽

Mauricio Pereira ◽

Andre Favoretto ◽

...

Keyword(s):

Shear Strength ◽

Tooth Movement ◽

Testing Machine ◽

Orthodontic Tooth Movement ◽

Skeletal Anchorage ◽

Bonding Agents ◽

Significant Difference ◽

Group 2 ◽

Student’S T ◽

Group 1

Introduction: Most miniplates used for skeletal anchorage lack built-in orthodontic devices. To address this issue, orthodontists must use creative solutions, such as bonding buttons, brackets, or tubes directly to the miniplates, thus making them more versatile devices that provide a wider range of tooth movement possibilities. The purpose of the present study was to ascertain the debonding strength in Megapascals (MPa) of orthodontic accessories bonded to skeletal anchorage miniplates with different bonding agents. Methods: Forty specimens were divided into two equal groups by bonding agent: Group 1, resin (Transbond XT®, 3M ESPE); Group 2, cyanoacrylate (Scotchbond®, 3M ESPE). Shear strength testing was performed in an EMIC DL-2000 universal testing machine. Results: The results obtained were 2.28 ± 0.44 MPa for Group 1 and 4.90 ± 0.76 MPa for Group 2. The Kolmogorov-Smirnov test was used to assess the normality of data distribution. Student's t-test was used to compare means in the response variable. Conclusion: A statistically significant difference was observed between groups. However, both bonding agents provided strength in excess of that needed for secure orthodontic tooth movement.

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Orthodontic Force–Induced BMAL1 in PDLCs Is a Vital Osteoclastic Activator

Journal of Dental Research ◽

10.1177/00220345211019949 ◽

2021 ◽

pp. 002203452110199

Author(s):

Y. Xie ◽

Q. Tang ◽

S. Yu ◽

W. Zheng ◽

G. Chen ◽

...

Keyword(s):

Bone Remodeling ◽

Alveolar Bone ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Nuclear Factor Κb ◽

Periodontal Ligament Cells ◽

Orthodontic Force ◽

Periodontal Tissues ◽

Alveolar Bone Remodeling ◽

Biomechanical Stimuli

Orthodontic tooth movement (OTM) depends on periodontal ligament cells (PDLCs) sensing biomechanical stimuli and subsequently releasing signals to initiate alveolar bone remodeling. However, the mechanisms by which PDLCs sense biomechanical stimuli and affect osteoclastic activities are still unclear. This study demonstrates that the core circadian protein aryl hydrocarbon receptor nuclear translocator–like protein 1 (BMAL1) in PDLCs is highly involved in sensing and delivering biomechanical signals. Orthodontic force upregulates BMAL1 expression in periodontal tissues and cultured PDLCs in manners dependent on ERK (extracellular signal–regulated kinase) and AP1 (activator protein 1). Increased BMAL1 expression can enhance secretion of CCL2 (C-C motif chemokine 2) and RANKL (receptor activator of nuclear factor–κB ligand) in PDLCs, which subsequently promotes the recruitment of monocytes that differentiate into osteoclasts. The mechanistic delineation clarifies that AP1 induced by orthodontic force can directly interact with the BMAL1 promoter and activate gene transcription in PDLCs. Localized administration of the ERK phosphorylation inhibitor U0126 or the BMAL1 inhibitor GSK4112 suppressed ERK/AP1/BMAL1 signaling. These treatments dramatically reduced osteoclastic activity in the compression side of a rat orthodontic model, and the OTM rate was almost nonexistent. In summary, our results suggest that force-induced expression of BMAL1 in PDLCs is closely involved in controlling osteoclastic activities during OTM and plays a vital role in alveolar bone remodeling. It could be a useful therapeutic target for accelerating the OTM rate and controlling pathologic bone-remodeling activities.

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