scholarly journals Bio-efficacy of Pseudomonas fluorescens isolated from chickpea fields as plant growth promoting rhizobacteria

2016 ◽  
Vol 9 (4) ◽  
pp. 138-146
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola

2017 ◽  
Vol 9 (1) ◽  
pp. 121-128
Author(s):  
S. Kumar ◽  
M. Singh ◽  
Sushil Sharma
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Jatropha Curcas ◽  
Root Rot ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Total Phenols ◽  
Phenyl Propanoid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.

Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant-growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase

2011 ◽  
Vol 57 (7) ◽  
pp. 578-589 ◽  
Author(s):  
Maqshoof Ahmad ◽  
Zahir A. Zahir ◽  
H. Naeem Asghar ◽  
M. Asghar
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Mung Bean ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Saline Stress ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Axenic Conditions

Twenty-five strains of plant-growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and 10 strains of rhizobia were isolated from rhizosphere soil samples and nodules of mung bean. They were screened in separate trials under salt-stressed axenic conditions. The three most effective strains of PGPR (Mk1, Pseudomonas syringae ; Mk20, Pseudomonas fluorescens ; and Mk25, Pseudomonas fluorescens biotype G) and Rhizobium phaseoli strains M1, M6, and M9 were evaluated in coinoculation for their growth-promoting activity at three salinity levels (original, 4 dS·m–1, and 6 dS·m–1) under axenic conditions. The results showed that salinity stress significantly reduced plant growth but inoculation with PGPR containing ACC deaminase and rhizobia enhanced plant growth, thus reducing the inhibitory effect of salinity. However, their combined application was more effective under saline conditions, and the combination Mk20 × M6 was the most efficient for improving seedling growth and nodulation. The effect of high ethylene concentrations on plant growth and the performance of these strains for reducing the negative impact of saline stress was also evaluated by conducting a classical triple-response bioassay. The intensity of the classical triple response decreased owing to inoculation with these strains, with the root and shoot lengths of inoculated mung bean seedlings increasing and stem diameter decreasing, which is a typical response to the dilution in a classical triple response bioassay. Thus, coinoculation with PGPR containing ACC deaminase and Rhizobium spp. could be a useful approach for inducing salt tolerance and thus improving growth and nodulation in mung bean under salt-affected conditions.

Cytokinin production by plant growth promoting rhizobacteria and selected mutants

2001 ◽  
Vol 47 (5) ◽  
pp. 404-411 ◽  
Author(s):  
Ines E García de Salamone ◽  
Russell K Hynes ◽  
Louise M Nelson
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Pure Culture ◽  
Growth Regulation ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Resistant Mutant ◽  
Plant Growth Promoting ◽  
Isopentenyl Adenosine ◽  
Growth Promoting

One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20–18, a rifampicin-resistant mutant (RIF), and two TnphoA-derived mutants (CNT1, CNT2), with reduced capacity to synthesize cytokinins, was further characterized in pure culture using immunoassay and thin layer chromatography. G20–18 produced higher amounts of three cytokinins, isopentenyl adenosine (IPA), trans-zeatin ribose (ZR), and DHZR than the three mutants during stationary phase. IPA was the major metabolite produced, but the proportion of ZR and DHZR accumulated by CNT1 and CNT2 increased with time. No differences were observed between strain G20–18 and the mutants in the amounts of indole acetic acid synthesized, nor were gibberellins detected in supernatants of any of the strains. Addition of 10–5 M adenine increased cytokinin production in 96- and 168-h cultures of strain G20–18 by approximately 67%. G20–18 and the mutants CNT1 and CNT2 may be useful for determination of the role of cytokinin production in plant growth promotion by PGPR.Key words: cytokinins, plant growth regulation, Pseudomonas fluorescens, rhizobacteria, plant growth promoting rhizobacteria (PGPR).

Effects of seed inoculation with a strain of Pseudomonas fluorescens on root growth and activity of wheat in well-watered and drought-stressed glass-fronted rhizotrons

1998 ◽  
Vol 78 (4) ◽  
pp. 545-551 ◽  
Author(s):  
K. M. Volkmar ◽  
E. Bremer
Keyword(s):  
Plant Growth ◽  
Root Growth ◽  
Pseudomonas Fluorescens ◽  
Crop Yields ◽  
Wheat Yield ◽  
Plant Growth Promoting Rhizobacteria ◽  
Triticum Aestivum L ◽  
Rooting Depth ◽  
Plant Growth Promoting ◽  
Growth Promoting

Identification of mechanisms by which plant growth-promoting rhizobacteria (PGPR) affect plants is required to obtain consistent or predictable increases in crop yields using PGPR. We conducted an outdoor lysimeter study using 12 glass-fronted rhizotrons to determine if inoculation with a strain of Pseudomonas fluorescens (G20-18) might benefit spring wheat (Triticum aestivum L.) (cv. Katepwa) yield by altering root growth. Inoculated and uninoculated treatments were either well watered or subjected to terminal drought with just one surface irrigation at anthesis. The only significant effect of G20-18 inoculation was a reduction in root growth in the well-watered rhizotrons after about 40 d. Inoculation did not significantly affect depth of rooting, soil moisture depletion, seed yield or uptake of 15N-labelled fertilizer. Watering regime did not affect overall root growth, although there were significant differences within specific depths. Final yields in well-watered treatments were double those of the drought-stressed treatments. Variability in yields within the drought-stressed treatments occurred due to accidental flooding of several rhizotrons early in the experiment, and demonstrated the value of deeply placed available moisture under drought conditions. We conclude that inoculation with G20-18 has minor effects on root growth that are unlikely to affect wheat yield. Key words: Plant growth-promoting rhizobacteria, rhizobacteria, roots, rooting depth, rhizotrons, N use efficiency, drought

scholarly journals APLIKASI PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) TERHADAP INFEKSI Chrysanthemum mild mottle virus (CMMV), PERTUMBUHAN, DAN PRODUKSI TANAMAN KRISAN (Chrysanthemum sp.)

2020 ◽  
Vol 3 (2) ◽  
pp. 31-49
Author(s):  
Fery Abdul Choliq ◽  
Mintarto Martosudiro ◽  
Safira Candra Jalaweni
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Plant Growth Promoting Rhizobacteria ◽  
Mottle Virus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Krisan (Chrysanthemum sp.) adalah tanaman florikultura yang memiliki nilai ekonomi tinggi dan berpotensi besar untuk dikembangkan. Budidaya krisan tidak lepas dari kendala penyakit yang menyebabkan penurunan produksi salah satunya adalah serangan penyakit Chrysanthemum mild mottle virus (CMMV) yang menyebabkan gejala rusaknya bunga, tanaman kerdil, dan malformasi. Pengendalian CMMV masih menggunakan insektisida yang bertujuan untuk menekan populasi serangga vektor. Pengurangan penggunaan insektisida dapat menggunakan alternatif pengendalian yang lebih aman yaitu Plant Growth Promoting Rhizobacteria (PGPR). Bacillus subtilis, Pseudomonas fluorescens, dan Azotobacter sp. merupakan rizobakteri yang biasa digunakan sebagai PGPR karena memiliki peranan yang penting dalam mengendalikan serangan patogen dan bermanfaat untuk memacu pertumbuhan dan produksi tanaman. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian PGPR isolat B. subtilis, P. fluorescens, Azotobacter sp., dan kombinasinya terhadap serangan CMMV, pertumbuhan, dan produksi pada tanaman krisan. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) 9 perlakuan. P0+: Kontrol positif (tanpa pemberian PGPR, dengan inokulasi virus); P0-: Kontrol negatif (tanpa pemberian PGPR, tanpa inokulasi virus); P1: Inokulasi virus + PGPR isolat B. subtilis; P2: Inokulasi virus + PGPR isolat P. fluorescens; P3: Inokulasi virus + PGPR isolat Azotobacter sp.; P4: Inokulasi virus + PGPR isolat B. subtilis + P. fluorescens; P5: Inokulasi virus + PGPR isolat B. subtilis + Azotobacter sp.; P6: Inokulasi virus + PGPR isolat    P. fluorescens + Azotobacter; P7:  Inokulasi virus + PGPR isolat P. fluorescens + B. subtilis + Azotobacter sp. Berdasarkan hasil penelitian, diketahui bahwa tanaman krisan yang diberi perlakuan PGPR tiga kombinasi yaitu P. fluorescens + B. subtilis + Azotobacter sp. menunjukkan hasil yang berbeda nyata dengan perlakuan yang lain. Setelah dikombinasikan, P. fluorescens + B. subtilis + Azotobacter sp. mampu memberikan hasil terbaik dalam hal menunda masa inkubasi CMMV, menekan intensitas serangan CMMV, serta meningkatkan pertumbuhan dan produksi tanaman krisan.

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