Domates Tohumlarında Pseudomonas syringae pv. tomato’ya Karşı Farklı Bor Bileşiklerinin Etkililikleri

Pseudomonas syringae pv. tomato, the bacterial agent for tomato speck disease, can cause serious epidemics with high leaf moisture, mild temperatures, and cultural practices allowing bacterial dissemination among host plants. Boron is an essential micro-nutrient for plant growth and health in agricultural production. In this study, the effectiveness of 14 different Boron compounds at 5 different doses (1, 5, 10, 20 and 40 mM) against P. s. pv. tomato at a concentration of 108 CFU ml-1 in vitro was evaluated and the most successful 4 different Boron compounds (Ammonium tetrafluoroborate, Sodium tetrafluoroborate, Zinc borate and Disodium octaborate tetrahydrate) were coated with 5 mM doses infected cv. H2274 tomato seeds to determine bacterial populations and seed emergence rates in the seeds. Among the 14 different Boron compounds used in the experiments, Disodium octaborate tetrahydrate was the most successful active ingredient 92% ratio in inhibiting P. s. pv. tomato populations in tomato seeds, while 39% success was achieved with sodium tetrafluoroborate applications. According to the results of the research, it is thought that some Boron compounds can be an economical, effective and environmentally friendly chemical in reducing P. s. pv. tomato in tomato seeds within the scope of good agricultural practices.

Formula padat Bacillus cereus STRAIN TLE1.1 untuk pengendalian penyakit busuk pangkal batang (Sclerotium rolfsii) pada tanaman tomat

Penyakit utama tanaman tomat yaitu busuk pangkal batang yang disebabkan oleh Sclerotium rolfsii dapat menimbulkan kerugian mencapai 80-100%. Tujuan penelitian yaitu untuk mendapatkan formula padat Bacillus cereus strain TLE1.1 yang efektif untuk pengendalian penyakit busuk pangkal batang pada tanaman tomat. Penelitian bersifat eksperimen dengan mengamati kemampuan formula padat B. cereus strain TLE1.1 dalam pengendalian penyakit busuk pangkal batang dengan Rancangan Acak Lengkap yang terdiri atas 9 perlakuan dan 3 ulangan. Perlakuan terdiri atas kombinasi bahan pembawa formula padat yang terdiri atas limbah padat ampas tebu, ampas tahu dan tongkol jagung, fungisida serta kontrol. Masing-masing formula padat B. cereus strain TLE1.1 diintroduksi pada benih dan bibit tomat. Hasil penelitian menunjukkan bahwa hampir semua formula mampu menekan penyakit busuk pangkal batang tanaman tomat. Formula terbaik dalam menurunkan penyakit busuk pangkal batang pada tanaman yaitu formula ampas tahu dan ampas tahu + tongkol jagung.Main disease of tomato plant, namely stem rot caused by Sclerotium rolfsii which can cause losses up to 80-100%. The aim of the study was to obtain a solid formula of Bacillus cereus strain TLE1.1 which was effective for controlling stem rot disease in tomato plant. This research was an experimental study to know the ability of the solid formula of B. cereus strain TLE1.1 in controlling stem rot disease which was carried out in a completely randomizeddesign consisting of 9 treatments and 3 replications. The treatment consistedof a combination of solid formula carriers consisting of sugarcane solid waste,tofu dreg and corncob, fungicides and controls. Each solid formula of B. cereus strain TLE1.1 was introduced into tomato seeds and seedlings. The results showed that almost all of the formulas were able to suppress stem base disease of tomato plants. The best formula that reduced stem rot in plants were the tofu dreg and tofu dreg + corncob formula.

Caliente a deterioração das sementes de tomate verde dependendo de sua imersão em água quente / The deterioration of green tomato seeds depending on their immersion in hot water

Objetivo deste estudo foi analisar a viabilidade das sementes de tomate verde (capacidade de germinar) coletadas em quatro jardins agroecológicos familiares em duas comunidades do Caminho Real de Becal e Calkiní, no Estado de Campeche, méxico. A técnica de imersão temporária em (0, 1,2,3,5,7 e 10 s) de 5400 sementes de tomate verde em água quente a 100°C determinou seu grau de deterioração no laboratório e no campo, indicado de acordo com a capacidade de germinar calculando o percentual de germinação; da mesma forma, foram medidos pH, CE, °Brix do suco celular. De acordo com os resultados houve um percentual de germinação de 96% no controle absoluto semelhante aos tempos de imersão de um, dois e três segundos, no entanto, nos tratamentos de quatro e cinco segundos de imersão reduziu para 72% e nos tratamentos de sete e 10 segundos as sementes não germinam em ambas as condições. O suco celular do tomate verde teve as seguintes médias de pH 3,51, condutividade elétrica 7,81 dSmen/cm2 e 5,65°Brix. Portanto, conclui-se que as sementes de tomate verde de quatro pomares agroecológicos de Becal e Calkiní apresentaram maior tolerância à deterioração por imersão em água quente a 100 ° C para 1, 2, 3, 5 s para atingir uma germinação acima de 90%. Houve comprometimento grave por imersão de 7 e 10 s. Portanto, as sementes podem manter o potencial em sua capacidade de germinação por três a cinco anos. Mas não por períodos acima de cinco anos onde +20% é perdido por ano semelhante aos resultados indicados pelos pesquisadores.

Discrimination of tomato seeds belonging to different cultivars using machine learning

This study was aimed at developing the discriminant models for distinguishing the tomato seeds based on texture parameters of the outer surface of seeds calculated from the images (scans) converted to individual color channels R, G, B, L, a, b, X, Y, Z. The seeds of tomatoes ‘Green Zebra’, ‘Ożarowski’, ‘Pineapple’, Sacher F1 and Sandoline F1 were discriminated in pairs. The highest results were observed for models built based on sets of textures selected individually from color channels R, L and X and sets of textures selected from all color channels. In all cases, the tomato seeds ‘Green Zebra’ and ‘Ożarowski’ were discriminated with the highest average accuracy equal to 97% for the Multilayer Perceptron classifier and 96.25% for Random Forest for color channel R, 95.25% (Multilayer Perceptron) and 95% (Random Forest) for color channel L, 93% (Multilayer Perceptron) and 95% (Random Forest) for color channel X, 99.75% (Multilayer Perceptron) and 99.5% (Random Forest) for a set of textures selected from all color channels (R, G, B, L, a, b, X, Y, X). The highest average accuracies for other pairs of cultivars reached 98.25% for ‘Ożarowski’ vs. Sacher F1, 95.75% for ‘Pineapple’ vs. Sandoline F1, 97.5% for ‘Green Zebra’ vs. Sandoline F1, 97.25% for Sacher F1 vs. Sandoline F1 for models built based on textures selected from all color channels. The obtained results may be used in practice for the identification of cultivar of tomato seeds. The developed models allow to distinguish the tomato seed cultivars in an objective and fast way using digital image processing. The results confirmed the usefulness of texture parameters of the outer surface of tomato seeds for classification purposes. The discriminative models allow to obtain a very high probability and may be applied to authenticate and detect seed adulteration.

Pre-Processing Unsellable Tomatoes into Separated Streams with the Intention of Recovering Protein

PurposeA large proportion of the European Union’s tomato crop is discarded during harvesting and there is a valorisation potential to recover proteins from this waste. MethodsCherry tomatoes were segregated into three separate components: juice, pomace (peels and skins), and seeds. The peels and skins, and seeds were separately hydrolyzed with carbohydrases to determine whether protein recovery could be increased. In addition, a strategy to fractionate the seeds using sequential washing of milled tomato seeds followed by low-speed centrifugation to remove the denser seed hulls and to collect the protein rich kernels remaining in suspension. ResultsThe protein content of the seeds was highest with 27.4% while the peels and skins contained 7.6%. Carbohydrase mediated hydrolysis revealed a minor increase in protein recovery from seeds by 10% using Filta 02L (cellulase, xylananse and β-glucanase), and the quantity of protein recovered from peels and skins increased by 210% using Tail 157 (pectinase, hemicellulase). The strategy to separate the seeds into two fractions, revealed that a higher proportion of the fibre (65%) was associated with the hull fraction compared with the original seeds (47%). A significant proportion of the fibre in this fraction was composed lignin although the protein contents between both fractions was similar ranging from 27.4% to 29.9%. ConclusionsThese results reveal that carbohydrases were quite effective in protein extraction from peels and skins, but not from seeds. An alternative strategy was developed to remove the seed hulls from the milled seeds and to collect a crude protein fraction where the protein content could be further improved.

Seed Priming With an Animal-derived Protein Hydrolysate Improves Drought Tolerance of Tomato Seeds by Enhancing Reserve Mobilization, Osmotic Adjustment, and Antioxidant Mechanism

Purpose Protein hydrolysates obtained from agro-industrial byproducts have received much attentions due to their positive roles in regulating plant responses to environmental stresses. However, little is known about the roles of animal protein hydrolysates in mediating seed drought tolerance and the underlying mechanism. Here, the effects of seed priming with pig blood protein hydrolysates (PP) on tomato seed germination and seedling growth under drought stress were investigated. Methods Tomato seeds were soaked with different concentrations of PP solutions for 24 h, and then transferred to filter paper moistened with distilled water or 10% PEG-6000 solution in Petri dish. The germination traits, seeding growth, reserve mobilization, osmolytes, and antioxidant system were determined.Results PP priming effectively alleviated the reduction in seed germination traits, resulting in improved tomato seedling growth under drought stress. PP priming enhanced amylase and sucrose synthase activities, soluble sugar, soluble protein, and free amino acid levels, thereby promoting reserve mobilization in seeds. Moreover, PP priming also reduces osmotic toxicity by increasing the accumulation of proline, soluble protein, and soluble sugar. Drought stress substantially enhanced the production of ROS and subsequent increases in MDA and Evans blue uptake, which were significantly alleviated after PP priming by improving the activities of SOD, POD, and CAT, and non-enzymatic antioxidants. Conclusion PP priming is a feasible method for improving tomato seed germination and seedling growth under drought stress by enhancing reserve mobilization, osmolyte accumulation, and antioxidant systems.

Lycoperoside H, a Tomato Seed Saponin, Improves Epidermal Dehydration by Increasing Ceramide in the Stratum Corneum and Steroidal Anti-Inflammatory Effect

Tomatoes are widely consumed, however, studies on tomato seeds are limited. In this study, we isolated 11 compounds including saponins and flavonol glycosides from tomato seeds and evaluated their effects on epidermal hydration. Among the isolated compounds, tomato seed saponins (10 µM) significantly increased the mRNA expression of proteins related to epidermal hydration, including filaggrin, involucrin, and enzymes for ceramide synthesis, by 1.32- to 1.91-fold compared with the control in HaCaT cells. Tomato seed saponins (10 µM) also decreased transepidermal water loss by 7 to 13 g/m2·h in the reconstructed human epidermal keratinization (RHEK) models. Quantitative analysis of the ceramide content in the stratum corneum (SC) revealed that lycoperoside H (1–10 µM) is a promising candidate to stimulate ceramide synthesis via the upregulation of ceramide synthase-3, glucosylceramide synthase, and β-glucocerebrosidase, which led to an increase in the total SC ceramides (approximately 1.5-fold) in concert with ceramide (NP) (approximately 2-fold) in the RHEK models. Evaluation of the anti-inflammatory and anti-allergic effects of lycoperoside H demonstrated that lycoperoside H is suggested to act as a partial agonist of the glucocorticoid receptor and exhibits anti-inflammatory effects (10 mg/kg in animal test). These findings indicate that lycoperoside H can improve epidermal dehydration and suppress inflammation by increasing SC ceramide and steroidal anti-inflammatory activity.

Osmo-priming in tomato seeds down-regulates genes associated with stress response and leads to reduction in longevity

Tomato seeds subjected to osmo-priming show fast and more uniform germination. However, osmo-priming reduces seed longevity, which is a complex seed physiological attribute influenced by several mechanisms, including response to stress. Thus, to have new insights as to why osmo-primed tomato seeds show a short life span, we performed a transcript analysis during their priming. For that, we performed gene expression studies of the heat-shock protein family genes that were previously reported to be associated with the enhancement of longevity in primed tomato seeds. Physiological assays of germination, vigour and longevity tests were used to support the data. The results show that the short life span of osmo-primed tomato seeds is related to the decrease in the expression of transcripts associated with response to stress during the priming treatment. These results are important because they add information regarding which seed longevity mechanisms are impacted by the priming treatment. In parallel, it will allow the use of these genes as markers to monitor longevity in osmo-primed tomato seeds.