scholarly journals Linoleic Acid Isomerase inLactobacillus plantarumAKU1009a Proved to Be a Multi-Component Enzyme System Requiring Oxidoreduction Cofactors

2011 ◽  
Vol 75 (2) ◽  
pp. 318-322 ◽  
Author(s):  
Shigenobu KISHINO ◽  
Jun OGAWA ◽  
Kenzo YOKOZEKI ◽  
Sakayu SHIMIZU
Keyword(s):  
Linoleic Acid ◽  
Enzyme System ◽  
Linoleic Acid Isomerase

Conjugated Linoleic Acid Production by Fermentation

2006 ◽  
Vol 2 (4) ◽  
Author(s):  
Ming Dong ◽  
Shuting Qi
Keyword(s):  
Linoleic Acid ◽  
Incubation Time ◽  
Lactobacillus Acidophilus ◽  
Seed Oil ◽  
Incubation Temperature ◽  
Conversion Ratio ◽  
Isomerase Activity ◽  
Whole Milk ◽  
Alfalfa Seed ◽  
Linoleic Acid Isomerase

Lactobacillus acidophilus 1.1854 was used for CLA production in whole milk and alfalfa seed oil was used as substrate. Alfalfa seed oil contained linoleic acid about 40%. Results showed that alfalfa seed oil addition to the culture improved CLA production, indicating the presence of linoleic acid isomerase activity in the culture. The concentration of lactic acid bacteria, the incubation time, the substrate concentration, the pH, incubation temperature, the pre-incubation time and the substrate amount of pre-incubation were studied in our research and they are optimized at 2.5%(v/v), 21h, 0.05%(v/v), pH 6.4, 37°C, 11h and 10µL which brought the optimal conversion ratio at about 50%.

Characterization of the triple-component linoleic acid isomerase inLactobacillus plantarumZS2058 by genetic manipulation

2017 ◽  
Vol 123 (5) ◽  
pp. 1263-1273 ◽  
Author(s):  
B. Yang ◽  
H. Qi ◽  
Z. Gu ◽  
H. Zhang ◽  
W. Chen ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Genetic Manipulation ◽  
Linoleic Acid Isomerase

Purification and characterization of a membrane-bound linoleic acid isomerase from Clostridium sporogenes

2007 ◽  
Vol 40 (4) ◽  
pp. 831-839 ◽  
Author(s):  
Susan S. Peng ◽  
Ming-De Deng ◽  
Alan D. Grund ◽  
Reinhardt A. Rosson
Keyword(s):  
Linoleic Acid ◽  
Purification And Characterization ◽  
Clostridium Sporogenes ◽  
Membrane Bound ◽  
Linoleic Acid Isomerase

Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina

2015 ◽  
Vol 37 (10) ◽  
pp. 1983-1992 ◽  
Author(s):  
Danhui Hao ◽  
Haiqin Chen ◽  
Guangfei Hao ◽  
Bo Yang ◽  
Baixi Zhang ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Heterologous Expression ◽  
Conjugated Linoleic Acid ◽  
Mortierella Alpina ◽  
Oleaginous Fungus ◽  
Linoleic Acid Isomerase

scholarly journals Linoleic acid isomerase gene FgLAI12 affects sensitivity to salicylic acid, mycelial growth and virulence of Fusarium graminearum

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ya-Zhou Zhang ◽  
Zhen-Zhen Wei ◽  
Cai-Hong Liu ◽  
Qing Chen ◽  
Bin-Jie Xu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Linoleic Acid ◽  
Fusarium Graminearum ◽  
Mycelial Growth ◽  
Linoleic Acid Isomerase

Heterologous Expression of Production of T10, C12-CLA Linoleic Acid Isomerase Gene from Propionibacterium acnes

2013 ◽  
Vol 641-642 ◽  
pp. 765-768
Author(s):  
Xue Luo ◽  
Lan Wei Zhang ◽  
Hong Bo Li ◽  
Shu Mei Wang ◽  
Chao Hui Xue
Keyword(s):  
Escherichia Coli ◽  
Linoleic Acid ◽  
Double Bond ◽  
Propionibacterium Acnes ◽  
Expression System ◽  
Fusion Partner ◽  
His Tag ◽  
Double Bond System ◽  
Bond System ◽  
Linoleic Acid Isomerase

The linoleic acid isomerase enzyme from Propionibacterium acnes, which catalyzed the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating trans-10, cis-12 conjugated linoleic acid (t10, c12 CLA) was cloned and overexpressed in Escherichia coli using expression system of pCold-SUMO. The gene was expressed under T7 promotor with a fusion partner of 6×His. Tag at its 5'terminal. After induction by 0.2mM IPTG for 18 h, the recombinant enzyme are expressing in the cytoplasm. The 1275 bp gene from P. acnes encode a linoleic acid isomerase protein with 424 amino acids (55KD).

Linoleic Acid Isomerase from Propionibacterium acnes: Purification, Characterization, Molecular Cloning, and Heterologous Expression

2007 ◽  
Vol 143 (3) ◽  
pp. 199-211 ◽  
Author(s):  
Ming-De Deng ◽  
Alan D. Grund ◽  
Kenneth J. Schneider ◽  
Kim M. Langley ◽  
Sarah L. Wassink ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Propionibacterium Acnes ◽  
Linoleic Acid Isomerase

scholarly journals Heterologous expression of linoleic acid isomerase from Propionibacterium acnes and anti-proliferative activity of recombinant trans-10, cis-12 conjugated linoleic acid

Microbiology ◽  
2007 ◽  
Vol 153 (8) ◽  
pp. 2483-2490 ◽  
Author(s):  
Eva Rosberg-Cody ◽  
Mark C. Johnson ◽  
Gerald F. Fitzgerald ◽  
Paul R. Ross ◽  
Catherine Stanton
Keyword(s):  
Linoleic Acid ◽  
Heterologous Expression ◽  
Conjugated Linoleic Acid ◽  
Proliferative Activity ◽  
Propionibacterium Acnes ◽  
Linoleic Acid Isomerase
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