scholarly journals Effect of Leukemia Inhibitory Factor Added to Maturation Medium on In Vitro Maturation, Fertilization and Development of Bovine Follicular Oocytes.

1997 ◽  
Vol 14 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Nao Araki ◽  
Eun Song Lee ◽  
Yutaka Fukui
Keyword(s):  
Leukemia Inhibitory Factor ◽  
In Vitro Maturation ◽  
Inhibitory Factor ◽  
Maturation Medium

scholarly journals In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

2020 ◽  
Vol 21 (19) ◽  
pp. 7067
Author(s):  
Meritxell Vendrell-Flotats ◽  
Tania García-Martínez ◽  
Iris Martínez-Rodero ◽  
Manel Lopez-Bejar ◽  
Jonathan LaMarre ◽  
...  
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Embryo Development ◽  
Leukemia Inhibitory Factor ◽  
In Vitro Maturation ◽  
Expression Patterns ◽  
Inhibitory Factor ◽  
Blastocyst Development ◽  
Developmental Potential

Oocyte cryopreservation has a significant impact on subsequent embryonic development. Herein, we investigated whether supplementing in vitro maturation medium with Leukemia Inhibitory Factor (LIF) prior to vitrification affects embryo development and gene expression at different embryo developmental stages. A panel of genes including maternal effect, epigenetics, apoptosis and heat stress was relatively quantified. The results show reduced cleavage rates after vitrification, regardless of the LIF treatment. Although not statistically different from control-vitrified oocytes, oocyte apoptosis and the blastocyst yield of LIF-vitrified oocytes were similar to their non-vitrified counterparts. Vitrification increased oocyte ZAR1, NPM2 and DPPA3 gene expression while its expression decreased in LIF-vitrified oocytes to similar or close levels to those of non-vitrified oocytes. With a few gene-specific exceptions, vitrification significantly increased the expression of DNMT3A, HDAC1, KAT2A, BAX and BCL2L1 in oocytes and most stages of embryo development, while comparable expression patterns for these genes were observed between LIF-vitrified and non-vitrified groups. Vitrification increased HSPA1A expression in oocytes and HSP90AA1 in 2-cell embryos. Our data suggest that vitrification triggers stage-specific changes in gene expression throughout embryonic development. However, the inclusion of LIF in the IVM medium prior to vitrification stimulates blastocyst development and several other developmental parameters and induces oocytes and embryos to demonstrate gene expression patterns similar to those derived from non-vitrified oocytes.

91 EFFECT OF LEUKEMIA INHIBITORY FACTOR (LIF) ON MATURATION OF PORCINE OOCYTES IN VITRO MATURATION AND DEVELOPMENT OF PARTHENOGENETIC EMBRYOS

2014 ◽  
Vol 26 (1) ◽  
pp. 159 ◽  
Author(s):  
Y. B. Choi ◽  
S. J. Kim ◽  
E. J. Park ◽  
K. Y. Song ◽  
J. H. Moon ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
In Vitro Maturation ◽  
Cell Number ◽  
Total Cell ◽  
Inhibitory Factor ◽  
Blastocyst Formation ◽  
Total Cell Number ◽  
Parthenogenetic Embryos ◽  
Cattle And Sheep

Cytokines have been suggested to have an important role for successful implantation. Among the cytokines, leukemia inhibitory factor (LIF) is a pleiotropic cytokine of the interlukin-6 family that has been confirmed for its significance in implantation in human and animal studies. Furthermore, it has been reported that LIF enhanced in vitro maturation (IVM) in cattle and sheep, blastocyst formation and hatching rate of embryos and pregnancy rate in mouse, human, cattle and sheep. However, to our knowledge, there has been no report on the effects of human LIF (hLIF) on pig oocytes IVM and embryos development. Therefore, we designed and performed this study to examine the effect of hLIF treatment on pig IVM and oocyte developmental competence. We investigated the effect of hLIF treatment during pig oocyte in vitro maturation (IVM) and in vitro culture (IVC) on parthenogenetic embryos. Three groups of different hLIF concentrations were used: 0, 0.5, and 1.0 ng mL–1. In experiment 1, hLIF was contained on IVM media, in experiment 2, hLIF was contained on IVC media for 7 days, and experiment 3, hLIF was contained on IVM and IVC media. In experiment 1, hLIF in IVM media significantly increased cleavage rate in 0.5 ng mL–1 group [hLIF 0 ng mL–1; 46.56 ± 3.1 (%), 0.5 ng mL–1; 58.43 ± 3.6 (%), 1.0 ng mL–1; 52.05 ± 2.7 (%)] and total cell number of blastocysts in hLIF 1.0 ng mL–1 group (hLIF 0 ng mL–1; 35.00 ± 2.3, 0.5 ng mL–1; 40.71 ± 3.1, 1.0 ng mL–1; 51.06 ± 3.7) but no significant differences were found in oocyte maturation rate or blastocyst formation rate. In experiment 2, total cell number of blastocysts showed significant difference in hLIF 1.0 ng mL–1 in IVC media (hLIF 0 ng mL–1; 43.81 ± 1.6, hLIF 0.5 ng mL–1; 45.97 ± 2.0, hLIF 1.0 ng mL–1; 52.10 ± 2.9). Finally, total cell number of blastocysts increased in hLIF 0.5 ng mL–1 in IVM and IVC media [hLIF 0 ng mL–1; 46.50 ± 2.3 (%), 0.5 ng mL–1; 55.11 ± 2.9 (%)]. In conclusion, hLIF supplementation to IVM and IVC medium improved porcine embryo development in terms of increasing total cell number of blastocysts. This study was supported by Korean MKE (#10033839), the Research Institute for Veterinary Science.

scholarly journals In vitro maturation in the presence of Leukemia Inhibitory Factor modulates gene and miRNA expression in bovine oocytes and embryos

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Meritxell Vendrell-Flotats ◽  
Tania García-Martínez ◽  
Iris Martínez-Rodero ◽  
Manel López-Béjar ◽  
Jonathan LaMarre ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Leukemia Inhibitory Factor ◽  
In Vitro Maturation ◽  
Cumulus Cells ◽  
Inhibitory Factor ◽  
Hatching Rate ◽  
Cleavage Rate ◽  
Oocyte Competence ◽  
Bovine Oocytes

Abstract Members of the interleukin-6 (IL-6) family of cytokines are important for reproductive function that are mediated through changes in gene and miRNA expression. Herein, we characterized the expression of miR-21, miR-155, miR-34c and miR-146a in bovine oocytes and cumulus cells during in vitro maturation (IVM) with leukemia inhibitory factor (LIF), IL-6 and IL-11 or unsupplemented controls. LIF-exposed COCs showed higher expression of miR-21 and miR-155 in oocytes, whereas miR-146a expression was increased in oocytes matured with IL-6 and IL-11. In cumulus cells, miR-155 expression was elevated by all treatments while only LIF increased miR-21 expression. Based on these results, we next examined how LIF exposure during IVM affected oocyte competence, through IVF and the expression of specific genes in GV- and MII-oocytes, in 2- and 8-cell embryos, and in Day 8-blastocysts. LIF supplementation did not affect cleavage rate, blastocyst yield or several other developmental parameters, but did increase hatching rate. LIF suppressed DPPA3, ZAR1 and NPM2 expression in 2 cell- and/or 8-cell embryos. LIF increased the expression of KAT2A and HSPA1A in MII-oocytes, and that of HDAC1, KAT2A and HSP90AA1 and the BAX:BCL2L1 ratio in 2-cell embryos. In contrast, HDAC1, KAT2A and HSP90AA1 expression and BAX:BCL2L1 ratio was lower in 8-cell embryos derived from LIF oocytes. IVM with LIF also increased the expression of DNMT3A, HSPA1A and HSP90AA1 in blastocysts. In conclusion, supplementation with LIF during IVM was consistently associated with changes in the relative abundance of transcripts in mature bovine oocytes and in specific embryo developmental stages.

Endometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization

2008 ◽  
Vol 102 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Paulo Serafini ◽  
André M. Rocha ◽  
Cyntia T. Osório ◽  
Ismael da Silva ◽  
Eduardo L. Motta ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Leukemia Inhibitory Factor ◽  
Inhibitory Factor ◽  
Vitro Fertilization

scholarly journals Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

2021 ◽  
Vol 14 (2) ◽  
pp. 452-456
Author(s):  
Mohamed Fathi ◽  
Amr F. Elkarmoty
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blue Staining ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Nuclear Maturation ◽  
Maturation Medium

Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5). Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively). Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.

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