Histological and Histomorphometric Studies of the Effects of Hyaluronic Acid on Osseointegration of Titanium Implant in Rabbits

2018 ◽  
Vol 30 (2) ◽  
pp. 10-16
Author(s):  
Mohammad Hasan Mohammad ◽  
Nada M. H. Al-Ghaban
Keyword(s):  
Hyaluronic Acid ◽  
Bone Formation ◽  
Healing Process ◽  
Pure Titanium ◽  
Titanium Implant ◽  
Commercially Pure Titanium ◽  
Histomorphometric Analysis ◽  
Natural Polymers ◽  
Commercially Pure ◽  
Significant Difference

Background: One of the unique prosthesis for tooth or teeth replacement is the dental implant. Our attempt is using a biomaterial system that is easily obtained and applicable and has the ability to provoke osteoinductive growth factor to enhance bone formation at the site of application. One of these natural polymers is hyaluronic acid. Material and methods: Sixty machined surface implants from commercially pure titanium rod inserted in thirty NewZealand rabbits. Two implants placed in both tibia of each rabbit. The animals scarified at 1, 2 and 4 weeks after implantation (10 rabbits for each interval). For all of animals the right tibia’s implant was control (uncoated) and the left one was experimental (coated with 0.1ml Hyaluronic acid gel). All sections have been stained with Haematoxylin and Eosin then they were histologically examined and assessed for histomorphometric analysis for counting of bone cells (osteoblast, osteocyte and osteoclast), cortical bone thickness, trabecular width, thread width and marrow space star volume (V*). Results: Histological findings for hyaluronic acid- coated titanium implant revealed an earlier bone formation, mineralization and maturation than that in control groups. Histomorphometric analysis for all bone parameters that examined in this study, showed highly significant difference between control and experimental groups in all healing intervals. Conclusion: Commercially pure titanium endosseous implants coated with hyaluronic acid may be osteocoductive thus accelerating healing process and enhancing osseointegration.

Histological and histomorphometric studies of the effects of hyaluronic acid on osseointegration of titanium implant in rabbits

2018 ◽  
Vol 30 (2) ◽  
pp. 10-16
Author(s):  
Mohammad H. Mohammad ◽  
Nada M.H. Al Ghaban
Keyword(s):  
Hyaluronic Acid ◽  
Bone Formation ◽  
Healing Process ◽  
Pure Titanium ◽  
Titanium Implant ◽  
Commercially Pure Titanium ◽  
Histomorphometric Analysis ◽  
Natural Polymers ◽  
Commercially Pure ◽  
Significant Difference

Background: One of the unique prosthesis for tooth or teeth replacement is the dental implant. Our attempt is using a biomaterial system that is easily obtained and applicable and has the ability to provoke osteoinductive growth factor to enhance bone formation at the site of application. One of these natural polymers is hyaluronic acid. Material and methods: Sixty machined surface implants from commercially pure titanium rod inserted in thirty NewZealand rabbits. Two implants placed in both tibia of each rabbit. The animals scarified at 1, 2 and 4 weeks after implantation (10 rabbits for each interval). For all of animals the right tibia’s implant was control (uncoated) and the left one was experimental (coated with 0.1ml Hyaluronic acid gel). All sections have been stained with Haematoxylin and Eosin then they were histologically examined and assessed for histomorphometric analysis for counting of bone cells (osteoblast, osteocyte and osteoclast), cortical bone thickness, trabecular width, thread width and marrow space star volume (V*). Results: Histological findings for hyaluronic acid- coated titanium implant revealed an earlier bone formation, mineralization and maturation than that in control groups. Histomorphometric analysis for all bone parameters that examined in this study, showed highly significant difference between control and experimental groups in all healing intervals. Conclusion: Commercially pure titanium endosseous implants coated with hyaluronic acid may be osteocoductive thus accelerating healing process and enhancing osseointegration.

scholarly journals Effect of fluoride sodium mouthwash solutions on cpTI: evaluation of physicochemical properties

2012 ◽  
Vol 23 (5) ◽  
pp. 496-501 ◽  
Author(s):  
Marcelo Bighetti Toniollo ◽  
Rodrigo Galo ◽  
Ana Paula Macedo ◽  
Renata Cristina Silveira Rodrigues ◽  
Ricardo Faria Ribeiro ◽  
...  
Keyword(s):  
Anodic Polarization ◽  
Titanium Surface ◽  
Pure Titanium ◽  
Distilled Water ◽  
Commercially Pure Titanium ◽  
Time Intervals ◽  
Commercially Pure ◽  
Significant Difference ◽  
Scanning Electron ◽  
Corrosion Data

The effects of fluoride, which is present in different oral hygiene products, deserve more investigation because little is known about their impact on the surface of titanium, which is largely used in Implantology. This study evaluated the surface of commercially pure titanium (cpTi) after exposure to different concentrations of sodium fluoride (NaF). The hypothesis tested in this study was that different concentrations of NaF applied at different time intervals can affect the titanium surface in different ways. The treatments resulted in the following groups: GA (control): immersion in distilled water; GB: immersion in 0.05% NaF for 3 min daily; GC: immersion in 0.2% NaF for 3 min daily; GD: immersion in 0.05% NaF for 3 min every 2 weeks; and GE: immersion in 0.2% NaF for 3 min every 2 weeks. The experiment lasted 60 days. Roughness was measured initially and every 15 days subsequently up to 60 days. After 60 days, corrosion analysis and anodic polarization were done. The samples were examined by scanning electron microscopy (SEM). The roughness data were analyzed by ANOVA and there was no significant difference among groups and among time intervals. The corrosion data (i corr) were analyzed by the Mann-Whitney test, and significant differences were found between GA and GC, GB and GC, GC and GD, GC and GE. SEM micrographs showed that the titanium surface exposed to NaF presented corrosion that varied with the different concentrations. This study suggests that the use of 0.05% NaF solution on cpTi is safe, whereas the 0.2% NaF solution should be carefully evaluated with regard to its daily use.

Structure of Composite Biocompatible Titania Coatings Modified with Hydroxyapatite Nanoparticles

2013 ◽  
Vol 787 ◽  
pp. 376-381 ◽  
Author(s):  
Aleksandr Aleksandrovich Fomin ◽  
Igor Vladimirovich Rodionov ◽  
Aleksey Borisovich Steinhauer ◽  
Marina Alekseevna Fomina ◽  
Andrey Mikhailovich Zakharevich ◽  
...  
Keyword(s):  
Pure Titanium ◽  
Morphological Characteristics ◽  
Titanium Implant ◽  
Commercially Pure Titanium ◽  
Hydroxyapatite Nanoparticles ◽  
Induction Heat ◽  
Induction Heat Treatment ◽  
Commercially Pure ◽  
Implant Coatings ◽  
Titania Coatings

The article describes prospective composite biocompatible titania coatings modified with hydroxyapatite nanoparticles and obtained on intraosseous implants fabricated from commercially pure titanium. Consistency changes of morphological characteristics and crystalline structure, mechanical properties and biocompatibility of experimental titanium implant coatings obtained by the combination of oxidation and surface modification with hydroxyapatite during induction heat treatment are defined.

scholarly journals Retention Strength of Cobalt-Chromium vs Nickel-Chromium Titanium vs CP Titanium in a Cast Framework Association of Removable Partial Overdenture

2011 ◽  
Vol 12 (3) ◽  
pp. 179-186
Author(s):  
Adriana Cristina Zavanelli ◽  
Recardo Alexandre Zavanelli ◽  
José Everaldo de Aquino Souza ◽  
Nelson Renato França Alves da Silva ◽  
Paulo Guilherme Coelho ◽  
...  
Keyword(s):  
Potential Risk ◽  
Artificial Saliva ◽  
Pure Titanium ◽  
Commercially Pure Titanium ◽  
Cobalt Chromium ◽  
Nickel Chromium ◽  
Commercially Pure ◽  
Significant Difference ◽  
Retention Strength

ABSTRACT Aim There is little information considering the framework association between cast clasps and attachments. The aim of this study was to evaluate the retention strength of frameworks match circumferential clasps and extra resilient attachment cast in three different alloys (cobalt-chromium, nickel-chromium titanium and commercially pure titanium), using two undercut (0.25 and 0.75 mm) and considering different period of time (0, 1/2, 1, 2, 3, 4 and 5 years). Methods Using two metallic matrices, representing a partially edentulous mandibular right hemiarch with the first molar crown, canine root and without premolars, 60 frameworks were fabricated. Three groups (n = 20) of each metal were cast and each group was divided into two subgroups (n = 10), corresponding the molar undercut of 0.25 mm and 0.75 mm. The nylon male was positioned at the matrix and attached to the acrylic resin of the prosthetic base. The samples were subjected to an insertion and removal test under artificial saliva environment. Results The data were analyzed and compared with ANOVAs and Tukey's test at 95% of probability. The groups cast in cobaltchromium and nickel-chromium-titanium had the highest mean retention strength (5.58 N and 6.36 N respectively) without significant difference between them, but statistically different from the group cast in commercially pure titanium, which had the lowest mean retention strength in all the periods (3.46 N). The association frameworks using nickel-chromium- titanium and cobalt-chromium could be used with 0.25 mm and 0.75 mm of undercut, but the titanium samples seems to decrease the retention strength, mainly in the 0.75 mm undercut. The circumferential clasps cast in commercially pure titanium used in 0.75 mm undercuts have a potential risk of fractures, especially after the 2nd year of use. Conclusion This in vitro study showed that the framework association between cast clasp and an extra resilient attachment are suitable to the three metals evaluated, but strongly suggest extra care with commercially pure titanium in undercut of 0.75 mm. Clinical significance Frameworks fabricated in Cp Ti tend to decrease in retentive strength over time and have a potential risk of fracture in less than 0.75 mm of undercut. How to cite this article Souza JEdA, da Silva NRFA, Coelho PG, Zavanelli AC, Ferracioli RCSR, Zavanelli RA. Retention Strength of Cobalt-Chromium vs Nickel-Chromium Titanium vs CP Titanium in a Cast Framework Association of Removable Partial Overdenture. J Contemp Dent Pract 2011;12(3):179-186.

The Effects of a Static Magnetic Field on Bone Formation Around a Sandblasted, Large-Grit, Acid-Etched–Treated Titanium Implant

2013 ◽  
Vol 39 (S1) ◽  
pp. 248-255 ◽  
Author(s):  
Richard Leesungbok ◽  
Su-Jin Ahn ◽  
Suk-Won Lee ◽  
Geon-Ho Park ◽  
Joo-Sung Kang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Histomorphometric Analysis ◽  
Contact Ratio ◽  
Neodymium Magnet ◽  
Significant Difference ◽  
Rabbit Tibia ◽  
Group 3 ◽  
The Mean

The purpose of this study was to compare the bone formation around commercial sandblasted, large-grit, acid-etched (SLA)–treated titanium implants with or without a neodymium magnet in a rabbit tibia through histomorphometric analysis. Commercial SLA-treated implants with or without neodymium magnets were placed in 10 rabbits. After incising the flat part of the rabbit's tibia and installation of the specimens of titanium implants, the nonmagnet group was stitched without magnet insertion. On the other hand, the magnet group was inserted with neodymium magnet, fixed with pattern resin, and stitched. At 3 and 6 weeks after surgery, the animals were sacrificed, and the specimens were obtained. Undecalcified specimens were prepared for histomorphometric analysis of the bone-to-implant contact ratio (BIC) and bone volume (BV). The histomorphometric findings of the cortical bone showed that the mean BVs of the magnet group (3 weeks, 75.99%; 6 weeks, 82.94%) were higher than those of the nonmagnet group (3 weeks, 74.58%; 6 weeks, 78.75%), but there were no significant differences between the 2 groups (P > .05). In the marrow bone, the mean BICs of the magnet group (3 weeks, 10.36%; 6 weeks, 10.41%) were higher than those of the nonmagnet group (3 weeks, 6.41%; 6 weeks, 7.36%). After 3 weeks of installation, there was a significant difference between the 2 groups (P < .05). In rabbit tibia, the SLA-treated titanium implants with a neodymium magnet can trigger faster early peri-implant bone formation than those without a magnet.

scholarly journals Effect of the use of die spacer on the marginal fit of copings cast in NiCr, NiCrBe and commercially pure titanium

2007 ◽  
Vol 18 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Natércia Carreira Soriani ◽  
Mônica Barbosa Leal ◽  
Silvana Maria Paulino ◽  
Valéria Oliveira Pagnano ◽  
Osvaldo Luiz Bezzon
Keyword(s):  
Metal Matrix ◽  
Pure Titanium ◽  
Optical Microscope ◽  
Commercially Pure Titanium ◽  
Marginal Fit ◽  
Type Iv ◽  
Commercially Pure ◽  
Significant Difference ◽  
Marginal Discrepancy ◽  
Die Spacer

The goal of this study was to evaluate the effect of using die spacers on the marginal fit of NiCr (M1) and NiCrBe (M2) alloys and commercially pure titanium (cpTi) (M3) copings cast by the lost wax technique. Using a metal matrix, 45 resin added extra hard type IV stone models were obtained for the fabrication of wax patterns under the following conditions: no die spacer (A), with one die spacer layer (B) and with two die spacer layers (C), with five repetitions for each condition (alloy x die). Each die was waxed and the wax patterns were invested as per manufacturer's instructions. Three wax patterns were embedded in each casting ring, each corresponding to one of the conditions. Each coping, seated to the metal matrix by a seating pressure standardizing device (SPSD), was taken to an optical microscope for measurement of marginal discrepancy. The obtained data (µm) were analyzed statistically by ANOVA and Tukey' test (a=5%). There was statistically significant difference (p<0.05) among the materials (M1=110.67; M2=130.33 and M3=148.33). Regarding the use of the die spacer, there was a statistically significant difference (p<0.05) among the three conditions (A=162.00; B=131.06 and C=96.67). It was concluded that there is less marginal discrepancy with two die spacer layers.

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