Enzyme-Linked Immunosorbent Assay for Detection of Feline Serum Amyloid A Protein by Use of Immunological Cross-Reactivity of Polyclonal Anti-Canine Serum Amyloid A Protein Antibody.

Traumatic brain injury (TBI) causes a wide variety of neuroinflammatory events. These neuroinflammatory events depend, to a greater extent, on the severity of the damage. Our previous studies have shown that the liver produces serum amyloid A (SAA) at high levels in the initial hours after controlled cortical impact (CCI) injury in mice. Clinical studies have reported detectable SAA in the plasma of brain injury patients, but it is not clear if SAA levels depend on TBI severity. To evaluate this question, we performed a mild to severe CCI injury in wild-type mice. We collected blood samples and brains at 1, 3, and 7 days after injury for protein detection by western blotting, enzyme-linked immunosorbent assay, or immunohistochemical analysis. Our results showed that severe CCI injury compared to mild CCI injury or sham mice caused an increased neuronal death, larger lesion volume, increased microglia/macrophage density, and augmented neutrophil infiltration. Furthermore, we found that the serum levels of SAA protein ascended in the blood in correlation with high neuroinflammatory and neurodegenerative responses. Altogether, these results suggest that serum SAA may be a novel neuroinflammation-based, and severity-dependent, biomarker for acute TBI.

Download Full-text

A Rapid Enzyme-Linked Immunosorbent Assay for Serum Amyloid a Using Sequence-Specific Antibodies

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000309 ◽

1993 ◽

Vol 30 (3) ◽

pp. 278-286 ◽

Cited By ~ 13

Author(s):

Martin-Tino Časl ◽

Anders Grubb

Keyword(s):

Immunosorbent Assay ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Enzyme Linked Immunosorbent Assay ◽

Microtitre Plate ◽

Amyloid A ◽

Diagnostic Potential ◽

Assay Time ◽

Reliable Marker ◽

Specific Rabbit

A microtitre plate based enzyme-linked immunosorbent assay for determining the concentration of serum amyloid A (SAA) is described. The method employs easily produced sequence-specific rabbit antibodies and the preferential absorption of SAA to polystyrene, which obviates the use of capture antibodies and allows an assay time of only 3 · 5 h, so that the diagnostic potential of the SAA level as a rapid and reliable marker for inflammation can be fully exploited. The assay has a working concentration range of 0·1–2500 mg/L, which embraces the known biological variation of the SAA concentration. The intra-assay coefficient of variation (CV) for SAA concentrations above 10 mg/L is between 1 · 6 and 3·3% and the inter-assay CV between 3·0 and 4·2%. Recovery of SAA added to serum is from 96 to 102%.

Download Full-text

Serum amyloid a protein as a potential biomarker in predicting acute onset and association with in-hospital death in acute aortic dissection

BMC Cardiovascular Disorders ◽

10.1186/s12872-019-1267-0 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Yuchen He ◽

Changcheng Ma ◽

Jia Xing ◽

Shiyue Wang ◽

Chao Ji ◽

...

Keyword(s):

Aortic Dissection ◽

Hospital Mortality ◽

Serum Amyloid A ◽

Acute Aortic Dissection ◽

Serum Amyloid ◽

Hospital Death ◽

Enzyme Linked Immunosorbent Assay ◽

Amyloid A ◽

Potential Biomarker ◽

Serum Amyloid A Protein

Abstract Background Acute aortic dissection (AAD) is a life-threatening disorder in vascular surgery with a high early mortality. Serum amyloid A (SAA) is a kind of acute-phase protein with a rapid diagnostic value in other diseases. However, the researches on the performance of SAA for the diagnosis of AAD is still lacking. This retrospective study aimed to evaluate the SAA levels and further explore its potential diagnostic role in AAD patients. Methods SAA levels were measured by enzyme-linked immunosorbent assay (ELISA) in 63 controls and 87 AAD patients. Laboratory examinations were also performed. And relative clinical information was collected from participants included in this study. Results SAA levels were significantly higher in AAD patients than those in healthy controls. SAA levels were independently associated with the risk of AAD. There was a positive significant correlation between SAA and C reactive protein (R = 0.442, and P = 0.001). Based on receiver-operating characteristic (ROC) analysis, the area under the curve (AUC) of SAA for the diagnosis of AAD were 0.942 with optimal cut-off points of 0.427 mg/L. For in-hospital mortality, the AUC of SAA were 0.732 with optimal cut-off points of 0.500 mg/L. According to logistic regression analysis, higher SAA levels represent a higher risk of in-hospital mortality (OR = 1.25; 95%CI: 1.07–1.47; P = 0.005). Conclusion Our findings demonstrated that SAA levels were significantly enhanced in AAD. SAA was closely correlated with inflammatory parameters and coagulation-related parameters in AAD. Furthermore, SAA could be a potential bio-marker for identifying AAD in the early diagnosis. Finally, SAA > 5.0 mg/L are independently related to AAD in-hospital mortality.

Download Full-text

Monoclonal Antibody-Based Sensitive Enzyme-Linked Immunosorbent Assay for Murine Serum Amyloid A

Journal of Immunoassay ◽

10.1080/01971529909349352 ◽

1999 ◽

Vol 20 (4) ◽

pp. 223-235 ◽

Cited By ~ 7

Author(s):

Toshiyuki Yamada ◽

Takeaki Fukuda ◽

Atsufumi Wada ◽

Yoshihisa Itoh

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Enzyme Linked Immunosorbent Assay ◽

Amyloid A

Download Full-text

Serum Amyloid A Protein as a Potential Biomarker in predicting acute onset and association with in-hospital death in acute aortic dissection

10.21203/rs.2.17288/v1 ◽

2019 ◽

Author(s):

Yuchen He ◽

Changcheng Ma ◽

Jia Xing ◽

Shiyue Wang ◽

Ji Chao ◽

...

Keyword(s):

Aortic Dissection ◽

Hospital Mortality ◽

Serum Amyloid A ◽

Acute Aortic Dissection ◽

Serum Amyloid ◽

Hospital Death ◽

Enzyme Linked Immunosorbent Assay ◽

Amyloid A ◽

Study Results ◽

Serum Amyloid A Protein

Abstract Background: Acute aortic dissection (AAD) is a life-threatening disorder in vascular surgery with a high early mortality. Serum amyloid A (SAA) is a kind of acute-phase protein with a rapid diagnostic value in other diseases. However, the researches on the performance of SAA for the diagnosis of AAD is still lacking. This retrospective study aimed to evaluate the SAA levels and further explore its potential diagnostic role in AAD patients. Methods: SAA levels were measured by enzyme-linked immunosorbent assay (ELISA) in 63 controls and 87 AAD patients. Laboratory examinations were also performed. And relative clinical information was collected from participants included in this study. Results: SAA levels were significantly higher in AAD patients than those in healthy controls. SAA levels were independently associated with the risk of AAD. There was a positive significant correlation between SAA and C reactive protein (R=0.442, and P=0.001). Based on receiver-operating characteristic (ROC) analysis, the area under the curve (AUC) of SAA for the diagnosis of AAD were 0.942 with optimal cut-off points of 0.427mg/L. For in-hospital mortality, the AUC of SAA were 0.732 with optimal cut-off points of 0.500mg/L. According to logistic regression analysis, higher SAA levels represent a higher risk of in-hospital mortality (OR=1.25; 95%CI: 1.07-1.47; P=0.005). Conclusion: Our findings demonstrated that SAA levels were significantly enhanced in AAD. SAA was closely correlated with inflammatory parameters and coagulation-related parameters in AAD. Furthermore, SAA could be a potential bio-marker for identifying AAD in the early diagnosis. Finally, SAA >5.0 mg/L are independently related to AAD in-hospital mortality.

Download Full-text

Serum Amyloid A Protein as a Potential Biomarker in predicting acute onset and association with in-hospital death in acute aortic dissection

10.21203/rs.2.17288/v2 ◽

2019 ◽

Author(s):

Yuchen He ◽

Changcheng Ma ◽

Jia Xing ◽

Shiyue Wang ◽

Ji Chao ◽

...

Keyword(s):

Aortic Dissection ◽

Hospital Mortality ◽

Serum Amyloid A ◽

Acute Aortic Dissection ◽

Serum Amyloid ◽

Hospital Death ◽

Enzyme Linked Immunosorbent Assay ◽

Amyloid A ◽

Study Results ◽

Serum Amyloid A Protein

Abstract Background: Acute aortic dissection (AAD) is a life-threatening disorder in vascular surgery with a high early mortality. Serum amyloid A (SAA) is a kind of acute-phase protein with a rapid diagnostic value in other diseases. However, the researches on the performance of SAA for the diagnosis of AAD is still lacking. This retrospective study aimed to evaluate the SAA levels and further explore its potential diagnostic role in AAD patients. Methods: SAA levels were measured by enzyme-linked immunosorbent assay (ELISA) in 63 controls and 87 AAD patients. Laboratory examinations were also performed. And relative clinical information was collected from participants included in this study. Results: SAA levels were significantly higher in AAD patients than those in healthy controls. SAA levels were independently associated with the risk of AAD. There was a positive significant correlation between SAA and C reactive protein (R=0.442, and P=0.001). Based on receiver-operating characteristic (ROC) analysis, the area under the curve (AUC) of SAA for the diagnosis of AAD were 0.942 with optimal cut-off points of 0.427mg/L. For in-hospital mortality, the AUC of SAA were 0.732 with optimal cut-off points of 0.500mg/L. According to logistic regression analysis, higher SAA levels represent a higher risk of in-hospital mortality (OR=1.25; 95%CI: 1.07-1.47; P=0.005). Conclusion: Our findings demonstrated that SAA levels were significantly enhanced in AAD. SAA was closely correlated with inflammatory parameters and coagulation-related parameters in AAD. Furthermore, SAA could be a potential bio-marker for identifying AAD in the early diagnosis. Finally, SAA >5.0 mg/L are independently related to AAD in-hospital mortality.

Download Full-text

Direct binding enzyme-linked immunosorbent assay (ELISA) for serum amyloid A (SAA)

Journal of Immunological Methods ◽

10.1016/0022-1759(89)90085-9 ◽

1989 ◽

Vol 125 (1-2) ◽

pp. 125-135 ◽

Cited By ~ 44

Author(s):

Jean D. Sipe ◽

Wayne A. Gonnerman ◽

Leland D. Loose ◽

Greta Knapschaefer ◽

Wen-Jian Xie ◽

...

Keyword(s):

Immunosorbent Assay ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Enzyme Linked Immunosorbent Assay ◽

Amyloid A ◽

Direct Binding

Download Full-text

Expression of serum amyloid A protein in the absence of the acute phase response does not reduce HDL cholesterol or apoA-I levels in human apoA-I transgenic mice

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)32143-x ◽

1999 ◽

Vol 40 (4) ◽

pp. 648-653 ◽

Cited By ~ 7

Author(s):

Hiroshi Hosoai ◽

Nancy R. Webb ◽

Jane M. Glick ◽

Uwe J.F. Tietge ◽

Matthew S. Purdom ◽

...

Keyword(s):

Transgenic Mice ◽

Acute Phase ◽

Acute Phase Response ◽

Serum Amyloid A ◽

Hdl Cholesterol ◽

Phase Response ◽

Serum Amyloid ◽

Amyloid A ◽

Serum Amyloid A Protein

Download Full-text

Aggregation of Mouse Serum Amyloid A Protein Was Promoted by Amyloid-Enhancing Factors with the More Genetically Homologous Serum Amyloid A

International Journal of Molecular Sciences ◽

10.3390/ijms22031036 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1036

Author(s):

Xuguang Lin ◽

Kenichi Watanabe ◽

Masahiro Kuragano ◽

Kiyotaka Tokuraku

Keyword(s):

Amino Acid ◽

Serum Amyloid A ◽

Animal Species ◽

Amino Acid Sequences ◽

Serum Amyloid ◽

Mouse Serum ◽

Aa Amyloidosis ◽

Basic Residue ◽

Amyloid A ◽

Serum Amyloid A Protein

Amyloid A (AA) amyloidosis is a condition in which amyloid fibrils characterized by a linear morphology and a cross-β structure accumulate and are deposited extracellularly in organs, resulting in chronic inflammatory diseases and infections. The incidence of AA amyloidosis is high in humans and several animal species. Serum amyloid A (SAA) is one of the most important precursor amyloid proteins and plays a vital step in AA amyloidosis. Amyloid enhancing factor (AEF) serves as a seed for fibril formation and shortens the onset of AA amyloidosis sharply. In this study, we examined whether AEFs extracted and purified from five animal species (camel, cat, cattle, goat, and mouse) could promote mouse SAA (mSAA) protein aggregation in vitro using quantum-dot (QD) nanoprobes to visualize the aggregation. The results showed that AEFs shortened and promoted mSAA aggregation. In addition, mouse and cat AEFs showed higher mSAA aggregation-promoting activity than the camel, cattle, and goat AEFs. Interestingly, homology analysis of SAA in these five animal species revealed a more similar amino acid sequence homology between mouse and cat than between other animal species. Furthermore, a detailed comparison of amino acid sequences suggested that it was important to mSAA aggregation-promoting activity that the 48th amino acid was a basic residue (Lys) and the 125th amino acid was an acidic residue (Asp or Glu). These data imply that AA amyloidosis exhibits higher transmission activity among animals carrying genetically homologous SAA gene, and may provide a new understanding of the pathogenesis of amyloidosis.

Download Full-text