Seroprevalence of Epstein-Barr Virus IgM Antibodies among HIV-Patients and Apparently Healthy Blood Donors Attending Ahmadu Bello University Teaching Hospital, Shika-Zaria, Nigeria

2016 ◽  
Vol 4 (5) ◽  
pp. 105-111
Author(s):  
Henry Gabriel Bishop ◽  
Olajumoke Omobolanle Adegoke
Keyword(s):  
Teaching Hospital ◽  
Epstein Barr Virus ◽  
Blood Donors ◽  
University Teaching ◽  
Hiv Patients ◽  
Barr Virus ◽  
Igm Antibodies ◽  
Epstein Barr ◽  
Apparently Healthy

Epstein-Barr Virus (EBV)-Antibody Profile in an Infant Congenitally Infected by the EBV

PEDIATRICS ◽  
1982 ◽  
Vol 70 (5) ◽  
pp. 823-824
Author(s):  
R. Shihman Chang
Keyword(s):  
Epstein Barr Virus ◽  
In Utero ◽  
Healthy Infant ◽  
Infected Infant ◽  
Initial Dilution ◽  
Barr Virus ◽  
Antibody Profile ◽  
Epstein Barr ◽  
Apparently Healthy

In 1973, Blankenship and I reported on an apparently healthy infant who was infected by the Epstein-Barr virus (EBV) in utero.1 In testing for EBV capsid (VCA) antibody in this infant, we found weak positive reactions with undiluted sera but negative reactions with sera diluted at 1/4. Because sera were generally tested at the initial dilution of 1/8 or 1/10 and were considered as negative for VCA antibody if there were no visible reactions,2,3 we concluded that the congenitally infected infant was EBV-seronegative. See table in the PDF file

scholarly journals Epstein-Barr virus transmission from a blood donor to an organ transplant recipient with recovery of the same virus strain from the recipient's blood and oropharynx

Blood ◽  
1996 ◽  
Vol 87 (2) ◽  
pp. 812-817 ◽  
Author(s):  
C Alfieri ◽  
J Tanner ◽  
L Carpentier ◽  
C Perpete ◽  
A Savoie ◽  
...  
Keyword(s):  
Cord Blood ◽  
Blood Donor ◽  
Epstein Barr Virus ◽  
Blood Donors ◽  
Barr Virus ◽  
Ebv Infection ◽  
Transplant Patients ◽  
Throat Wash ◽  
Transforming Activity ◽  
Epstein Barr

A previous study (Savoie et al, Blood 83:2715, 1994) identified eight transplant patients who acquired Epstein-Barr virus (EBV) infection during the peritransplant period. Three of these patients subsequently developed B-cell lymphoproliferative disease within 4 months of transplantation. Among these, there was a 16-year-old liver transplant patient who was negative for EBV at the time of transplant and who received an EBV-negative organ. After transplant, this patient was transfused with 9 U of packed red blood cells. Eight of the donors were EBV-positive and one was EBV-negative. We succeeded in obtaining spontaneous lymphoblastoid cell lines (LCLs) from the blood of three of these donors, one of whom also yielded a cord-blood line established with his throat-wash EBV. Blood from a fourth donor did not yield an LCL, but his throat washing did have transforming activity when inoculated onto cord-blood leukocytes. We initially could establish spontaneous LCLs only from the recipient's blood. However, a throat- wash sample taken 11 weeks later did show transforming activity. The recipient was shown to have acquired the EBV infection from one of eight EBV-seropositive blood donors. Analysis of fragment length polymorphisms after polymerase chain reaction amplification of the EBV BamHI-K fragment was used to establish strain identity. Western blot analysis for existence of size polymorphisms in three classes of Epstein-Barr nuclear antigens (EBNA-1, EBNA-2, and EBNA-3) confirmed the DNA results. It is noteworthy that the blood donor responsible for transmitting his EBV strain to the recipient had experienced clinical infectious mononucleosis 15 months before donating blood. Our results may, thus, indicate a requirement for leukodepletion of blood destined for immunosuppressed EBV-negative patients. Finally, blood donors with a recent history of infectious mononucleosis should probably be identified so that their blood is not given to EBV-negative transplant patients.

scholarly journals Molecular diagnosis of cytomegalovirus, Epstein-Barr virus and Herpes virus 6 among blood donors in Ouagadougou, Burkina Faso

2014 ◽  
Vol 14 (S2) ◽  
Author(s):  
Lassina Traore ◽  
Issoufou Tao ◽  
Cyrille Bisseye ◽  
Florencia Djigma ◽  
Djénéba Ouermi ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Molecular Diagnosis ◽  
Epstein Barr Virus ◽  
Herpes Virus ◽  
Blood Donors ◽  
Barr Virus ◽  
Epstein Barr ◽  
Herpès Virus

Effects of Anti-Retroviral Drugs to Expression of Cell Surface Adhesion Molecules and Hypermutation of Immunoglobulin Heavy Chain Variable Gene of HIV-Related Lymphoma

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5280-5280
Author(s):  
Atsushi Sasakawa ◽  
Yasuhiro Maeda ◽  
Chikara Hirase ◽  
Akihisa Kanamaru
Keyword(s):  
Hiv Infection ◽  
Adhesion Molecules ◽  
Epstein Barr Virus ◽  
Facs Analysis ◽  
Hiv Patients ◽  
Barr Virus ◽  
Normal Individuals ◽  
Variable Gene ◽  
Epstein Barr ◽  
Growth Ability

Abstract It has been known that non-Hodgkin’s lymphomas (NHL) are a major complication in human immunodeficiency virus (HIV) infection, and a major cause of death in HIV infected patients. Recently, it has been reported that highly active anti-retroviral therapy (HAART) declines the incidence of HIV-related lymphoma. In this study, we tried to make in vitro model of HIV-related lymphoma by Epstein-Barr virus transformation, and to examine the cellular and molecular characteristics of the HIV-related lymphoma cells derived from patients with HIV infection. PBMCs from 10 patients HIV infection and 10 HIV-negative normal individuals were obtained by Ficoll-Paque (Amersham Biosciences Corp. NJ) density gradient centrifugation. Lymphoblastoid cell lines (LCL) were obtained from each patient through transformation with Epstein-Barr virus (B95-8). Cells were cultured with RPMI 1640 medium (GIBCO, Grand Island, NY) supplemented with 10% fetal calf serum (FCS) (JRH Bioscences, Inc. Lenexa, Kansas), antibiotics, and L-glutamine and maintained at 37°C in an atmosphere containing 5% CO2. FACS analysis revealed the cells to be CD3−, CD4−, CD8−, CD5−, CD19+, CD20+, HLA-class I+, -class II+. After the establishment of LCLs, to obtain a highly purified population, CD19+LCLs were sorted on a FACS Vantage SE (Becton Dickinson, San Jose, CA). The purity of each CD19+LCLs population evaluated by FACS analysis was always higher than 98%. After growth advantage of LCLs, growth ability of the LCLs from HIV patients was examined by MTT assay. In results, there is no significant difference of growth ability between LCLs derived from HIV patients and those derived from normal individuals. Furthermore, the expression of cell adhesion molecules (CD11a, CD11b, CD18, CD50, and CD54) of LCLs was observed by FACS. The results showed that the expression of CD18 on LCLs derived from HIV patients was enhanced significantly in compared to those of normal LCLs. This phenomenon seems to be consistent with development of primary central nervous system (CNS) lymphoma. Furthermore, effects of anti-retroviral drugs to the expression of the adhesion molecules are examined. Somatic hypermutation of immunoglobulin heavy chain variable gene (IGHV) has been detected over 90% of HIV-related lymphoma. The effects of HAART to hypermuation of IGHV of LCL derived from HIV patients will be addressed.

scholarly journals Transient Headache and Neurological Deficits with Cerebrospinal Fluid Lymphocytosis Associated with IgM Antibodies to the Epstein-Barr Virus Viral Capsid Antigen

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Kossivi Apetse ◽  
Ludovic Breynaert ◽  
Chloe Butaud ◽  
Albert Beschet ◽  
Karine Blanc-Lasserre ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Epstein Barr Virus ◽  
Immunological Response ◽  
Neurological Deficits ◽  
Viral Capsid ◽  
Viral Agent ◽  
Viral Capsid Antigen ◽  
Barr Virus ◽  
Igm Antibodies ◽  
Epstein Barr

Some authors have suggested that the syndrome of transient headache and neurological deficits with cerebrospinal fluid lymphocytosis (HaNDL) results from an immunological response directed against a viral agent. Here we report a case of HaNDL in an immunocompetent 19-year-old male that could support this hypothesis.

scholarly journals The positivity rate of Epstein-Barr virus anti-viral capsid antigen IgG among children with infectious mononucleosis in Diyala-Iraq

2020 ◽  
Vol 13 (3) ◽  
pp. 019-024
Author(s):  
Mohammad Kassem Saleh ◽  
Abdulrazak Shafiq Hasan ◽  
Nadhim Ghazal Noaman
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Age Group ◽  
Barr Virus ◽  
Control Subjects ◽  
Insignificant Difference ◽  
Epstein Barr ◽  
And Control ◽  
Mean Concentration ◽  
Apparently Healthy

Background: Epstein-Barr virus (EBV) is a double-stranded linear DNA human herpesvirus that is transmitted primarily through saliva during childhood. Although the majority of primary EBV infections are clinically asymptomatic, clinical cases are presented as infectious mononucleosis (IMN) syndrome. Objectives: This study was conducted to explore the rate of EBV anti-VCA IgG among children who were clinically suspected as having IMN in Diyala province. Subjects and methods: This is a cross sectional study that was carried out during 2018 in Diyala province- Iraq. A total of 370 blood samples were collected from 190 children under 15 years of age who were clinically suspected as having IMN, and 180 apparently healthy children as controls. The anti EBV VCA IgG antibodies were detected in serum using the VCA IgG ELISA kit (from Dia.Pro Diagnostic Bioprobes srl - Italy). Statistical analysis was carried out using the SPSS- version 25. A statistical significance was considered whenever the P value was ≤ 0.05. Results: The results showed that the IgG positivity rate among suspected IMN patients was insignificantly higher in the age group 10-14 years old children (80.8%, P = 0.364). In control subjects the highest positivity rate was in the age group of 1-4 years with a statistically significant difference (79.5%, P = 0.002). In suspected IMN patients, the age group of 10-14 years had the highest mean concentration ± SD of anti-VCA IgG (44.018 ± 38.644 arbitrary units per milliliter (arbU/ml)), while in controls, the highest value (38.018 ± 34.908 arbU/ml) was in the age group of 1-4 years, with insignificant difference in either group (P = 0.257 and 0.072, respectively). The results also showed that in both suspected IMN patients and control subjects, females showed higher IgG positivity rate (70.6%, and 75.5%) compared to males (64.8%, and 65.1%) with insignificant difference in both groups (P = 0.392 and 0.126, respectively). Similarly, the IgG mean concentration ± SD was insignificantly higher in females in both suspected IMN patients and control subjects (P = 0.447 and 0.256, respectively). 21 (87.5%) IgM positive suspected IMN patients were also IgG positive with a statistically significant association (P = 0.028). Conclusion: The positivity rate of anti-EBV VCA IgG among apparently healthy subjects in Diyala province was 70.6%, and the rate increases with age, with slight association with clinical suspicion of infectious mononucleosis.

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