scholarly journals Prosopis juliflora Bark - An Alternate Feedstock in the Production of Bioethanol using Thermo Tolerant Yeast Kluyveromyces marxianus

2017 ◽  
Vol 14 (3) ◽  
pp. 945-951
Author(s):  
S. Sivarathnakumar ◽  
G. Baskar ◽  
B. Bharathiraja ◽  
R. Praveen Kumar ◽  
S. Chozhavendhan ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reducing Sugars ◽  
Simultaneous Saccharification And Fermentation ◽  
Prosopis Juliflora ◽  
Lignocellulosic Material ◽  
Alternative Source ◽  
Inoculum Concentration ◽  
Perennial Plant ◽  
Pre Treatment ◽  
Simultaneous Saccharification

ABSTRACT: Prosopis juliflora, a widely available perennial plant can be an alternative source to sugar-containing feedstock, which can be considered as a prospective lignocellulosic material for bioethanol production. In the present study, bark of Prosopis juliflora was subjected to hydrothermal coupled with nitric acid pre-treatment (3%(v/v)) followed by sonication. The composition of cellulose, hemicellulose, lignin, reducing sugars and inhibitors at each stage of pre-treatment were analysed. Further, delignified lignocellulosic biomass was subjected to Simultaneous Saccharification and Fermentation (SSF) studies using Kluyveromyces marxianus (MTCC 1389) and commercial cellulase enzyme. The effect of operating parameters such as pH, temperature, substrate concentration and inoculum concentration were investigated and found to be 4.9, 41oC, 3% v/v and 5% w/v respectively. The maximum bioethanol concentration achieved by fermentation of woody stem Prosopis juliflora using the yeast was found to be 21.45g/l.

scholarly journals Life Cycle Analysis of Integrated Production of Ferulic Acid, 2,3 Butanediol and Microbial Plant Biostimulants from Lignocellulosic Biomass by a Two-Step Cascading Process

Proceedings ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 16
Author(s):  
Valentin Zamfiropol-Cristea ◽  
Lucian Vlădulescu ◽  
Diana Constantinescu-Aruxandei ◽  
Florin Oancea
Keyword(s):  
Life Cycle Analysis ◽  
Simultaneous Saccharification And Fermentation ◽  
Deep Eutectic Solvents ◽  
Feruloyl Esterase ◽  
Microbial Consortia ◽  
Small Scale ◽  
One Pot ◽  
Natural Deep Eutectic Solvents ◽  
Pre Treatment ◽  
Simultaneous Saccharification

A small scale biorefinery process, which includes two main steps: (1) biomass pre-treatment with natural deep eutectic solvents (NADES) and feruloyl-esterase (FAE); and (2) one pot production of a versatile chemical, 2,3 butanediol (2,3-BD), from NADES and FAE pre-treated lignocellulose biomass, by simultaneous saccharification and fermentation (SSF), performed by a plant biostimulant microbial consortia, was developed. [...]

scholarly journals Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 908 ◽  
Author(s):  
Tran Giang ◽  
Siriporn Lunprom ◽  
Qiang Liao ◽  
Alissara Reungsang ◽  
Apilak Salakkam
Keyword(s):  
Hydrogen Production ◽  
Reducing Sugars ◽  
Simultaneous Saccharification And Fermentation ◽  
Hydrogen Yield ◽  
Microalgal Biomass ◽  
Fermentation Time ◽  
Chlorella Sp ◽  
Volatile Solids ◽  
Filter Paper Unit ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass−1 of cellulase, 92 U g-biomass−1 of amylase, and 120 U g-biomass−1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H2 g-volatile-solids−1 (VS), with a productivity of 1.6 mL-H2 g-VS−1 h−1. Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production of 1.8 g/L of reducing sugars, leading to a hydrogen yield (HY) of 172 mL-H2 g-VS−1. Using PSSF, the fermentation time was shortened by 36 h in which a productivity of 2.4 mL-H2 g-VS−1 h−1 was attained. To the best of our knowledge, the present study is the first report on the use of SSF and PSSF for hydrogen production from microalgal biomass, and the HY obtained in the study is by far the highest yield reported. Our results indicate that PSSF is a promising process for hydrogen production from microalgal biomass.

scholarly journals Impacts of lignin contents and yeast extract addition on the interaction between spruce pulps and crude recombinant Paenibacillus endoglucanase

BioResources ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 853-866
Author(s):  
Chun-Han Ko ◽  
Fang-Jing Chen ◽  
Wan-Jyung Liao ◽  
Tzenge-Lien Shih
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Rate Constants ◽  
Degradation Rate ◽  
Reducing Sugars ◽  
Simultaneous Saccharification And Fermentation ◽  
Degree Of Polymerization ◽  
Fermentation Processes ◽  
Gain Access ◽  
Simultaneous Saccharification

Crude recombinant Paenibacillus endoglucanase was employed to investigate its ability to gain access into and to degrade spruce pulps having different lignin and pentosan contents. Since yeast extract is commonly present in the simultaneous saccharification and fermentation processes as a nitrogen source, its effect on the accessibility and degradability of crude endoglucanase was examined. Pulps with more lignin contents adsorbed more overall proteins. More protein impurities other than the recombinant Paenibacillus endoglucanase were found to be preferentially adsorbed on the surfaces of pulp with higher lignin contents. The addition of yeast extracts further enhanced the above trends, which might reduce the non-productive binding by pulp lignin. Pulps with more lignin contents were more difficult to be degraded by the crude endoglucanase; the reductions of degree of polymerization (DP) for pulps were more sensitive to the dosage of endoglucanase applied. The presence of yeast extracts increased the DP degradation rate constants, but decreased the release of reducing sugars during hydrolysis for pulp with higher lignin contents.

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