scholarly journals Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 908 ◽  
Author(s):  
Tran Giang ◽  
Siriporn Lunprom ◽  
Qiang Liao ◽  
Alissara Reungsang ◽  
Apilak Salakkam
Keyword(s):  
Hydrogen Production ◽  
Reducing Sugars ◽  
Simultaneous Saccharification And Fermentation ◽  
Hydrogen Yield ◽  
Microalgal Biomass ◽  
Fermentation Time ◽  
Chlorella Sp ◽  
Volatile Solids ◽  
Filter Paper Unit ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass−1 of cellulase, 92 U g-biomass−1 of amylase, and 120 U g-biomass−1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H2 g-volatile-solids−1 (VS), with a productivity of 1.6 mL-H2 g-VS−1 h−1. Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production of 1.8 g/L of reducing sugars, leading to a hydrogen yield (HY) of 172 mL-H2 g-VS−1. Using PSSF, the fermentation time was shortened by 36 h in which a productivity of 2.4 mL-H2 g-VS−1 h−1 was attained. To the best of our knowledge, the present study is the first report on the use of SSF and PSSF for hydrogen production from microalgal biomass, and the HY obtained in the study is by far the highest yield reported. Our results indicate that PSSF is a promising process for hydrogen production from microalgal biomass.

scholarly journals Improvement of hydrogen production from Chlorella sp. biomass by acid-thermal pretreatment

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6637 ◽  
Author(s):  
Tran T. Giang ◽  
Siriporn Lunprom ◽  
Qiang Liao ◽  
Alissara Reungsang ◽  
Apilak Salakkam
Keyword(s):  
Hydrogen Production ◽  
High Growth ◽  
High Growth Rate ◽  
Hydrogen Yield ◽  
Thermal Pretreatment ◽  
Optimum Conditions ◽  
Acid Pretreatment ◽  
Microalgal Biomass ◽  
Chlorella Sp ◽  
Pretreated Biomass

Background Owing to the high growth rate, high protein and carbohydrate contents, and an ability to grow autotrophically, microalgal biomass is regarded as a promising feedstock for fermentative hydrogen production. However, the rigid cell wall of microalgae impedes efficient hydrolysis of the biomass, resulting in low availability of assimilable nutrients and, consequently, low hydrogen production. Therefore, pretreatment of the biomass is necessary in order to achieve higher hydrogen yield (HY). In the present study, acid-thermal pretreatment of Chlorella sp. biomass was investigated. Conditions for the pretreatment, as well as those for hydrogen production from the pretreated biomass, were optimized. Acid pretreatment was also conducted for comparison. Results Under optimum conditions (0.75% (v/v) H2SO4, 160 °C, 30 min, and 40 g-biomass/L), acid-thermal pretreatment yielded 151.8 mg-reducing-sugar/g-biomass. This was around 15 times that obtained from the acid pretreatment under optimum conditions (4% (v/v) H2SO4, 150 min, and 40 g-biomass/L). Fermentation of the acid-thermal pretreated biomass gave 1,079 mL-H2/L, with a HY of 54.0 mL-H2/g-volatile-solids (VS), while only 394 mL/L and 26.3 mL-H2/g-VS were obtained from the acid-pretreated biomass. Conclusions Acid-thermal pretreatment was effective in solubilizing the biomass of Chlorella sp. Heat exerted synergistic effect with acid to release nutrients from the biomass. Satisfactory HY obtained with the acid-thermal pretreated biomass demonstrates that this pretreatment method was effective, and that it should be implemented to achieve high HY.

scholarly journals Impacts of lignin contents and yeast extract addition on the interaction between spruce pulps and crude recombinant Paenibacillus endoglucanase

BioResources ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 853-866
Author(s):  
Chun-Han Ko ◽  
Fang-Jing Chen ◽  
Wan-Jyung Liao ◽  
Tzenge-Lien Shih
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Rate Constants ◽  
Degradation Rate ◽  
Reducing Sugars ◽  
Simultaneous Saccharification And Fermentation ◽  
Degree Of Polymerization ◽  
Fermentation Processes ◽  
Gain Access ◽  
Simultaneous Saccharification

Crude recombinant Paenibacillus endoglucanase was employed to investigate its ability to gain access into and to degrade spruce pulps having different lignin and pentosan contents. Since yeast extract is commonly present in the simultaneous saccharification and fermentation processes as a nitrogen source, its effect on the accessibility and degradability of crude endoglucanase was examined. Pulps with more lignin contents adsorbed more overall proteins. More protein impurities other than the recombinant Paenibacillus endoglucanase were found to be preferentially adsorbed on the surfaces of pulp with higher lignin contents. The addition of yeast extracts further enhanced the above trends, which might reduce the non-productive binding by pulp lignin. Pulps with more lignin contents were more difficult to be degraded by the crude endoglucanase; the reductions of degree of polymerization (DP) for pulps were more sensitive to the dosage of endoglucanase applied. The presence of yeast extracts increased the DP degradation rate constants, but decreased the release of reducing sugars during hydrolysis for pulp with higher lignin contents.

Optimization of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Cotton Stalk

2013 ◽  
Vol 724-725 ◽  
pp. 391-398
Author(s):  
Qin Zhang ◽  
Yan Bin Li ◽  
Zhan Wen Liu ◽  
Yun Feng Pu ◽  
Li Ming Xia
Keyword(s):  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Ph Value ◽  
Raw Material ◽  
Cotton Stalk ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Experimental Yield ◽  
Simultaneous Saccharification

Steam-exploded cotton stalk was used as raw material in ethanol production through simultaneous saccharification and fermentation by Penicillium Q59 and Saccharomyces cerevisiae P1. The fermentative conditions were firstly examined by single factor experiments to determine the central point in Box-Behnken design, which was explored with expectation to get optimized fermentative conditions for enhancement of ethanol production. The results of optimized fermentative conditions were determined as follows: fermentation time was 10.5 days, bran added percent was 15%, initial pH value was 5.5. Under the optimal conditions, the experimental yield of ethanol was 99.85 ± 4.21 g·kg-1SECS (steam-exploded cotton stalk), which was close to the theoretical predicting value, it showed the model was feasible. The research results will provide technical reference for further exploitation of cotton stalk.

scholarly journals PENGARUH WAKTU DAN NUTRIEN PADA PROSES FERMENTASI SAMPAH ORGANIK MENJADI BIOETANOL DENGAN METODE SSF

EnviroUS ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 76-82
Author(s):  
Adha Ilmi Nuraini ◽  
Naniek Ratni J.A.R
Keyword(s):  
Organic Waste ◽  
Simultaneous Saccharification And Fermentation ◽  
Raw Materials ◽  
Vegetable Waste ◽  
Fermentation Time ◽  
Fermentation Processes ◽  
Fermentation Method ◽  
Ethanol Content ◽  
Oil Resources ◽  
Simultaneous Saccharification

Bioethanol is a renewable and environmentally friendly energy source that can overcome the depletion of oil resources in Indonesia and reduce the increase in greenhouse gases. Organic waste can be one of the raw materials for bioethanol production because of its abundant availability. This research was conducted to determine the potential for organic waste to be processed into bioethanol. This study used vegetable waste, fruit waste, and leaf waste and then fermented using yeast containing Saccharomyces c. The addition of nutrients (urea and NPK) and fermentation time for 3 days, 5 days, and 7 days were carried out to determine the effect on the resulting ethanol content. The production of bioethanol is carried out using the SSF (Simultaneous Saccharification and Fermentation) method so that the hydrolysis and fermentation processes occur simultaneously. The results showed that the highest ethanol content was 18.79% with a fermentation time of 5 days and the addition of 25 gr urea. The addition of nutrients and fermentation time affects the ethanol levels produced.

scholarly journals Improvement of Polymer Grade L-Lactic Acid Production Using Lactobacillus rhamnosus SCJ9 from Low-Grade Cassava Chips by Simultaneous Saccharification and Fermentation

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1143
Author(s):  
Kridsada Unban ◽  
Narongsak Puangkhankham ◽  
Apinun Kanpiengjai ◽  
Rasiravathanahalli Kaveriyappan Govindarajan ◽  
Dharman Kalaimurugan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Production Efficiency ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Low Grade ◽  
Scale Production ◽  
Fermentation Time ◽  
Simultaneous Saccharification

The present study aims to examine the process for L-lactic acid production from low-grade cassava chips (LGC) using a two-step fermentation approach (TSF) and simultaneous saccharification and fermentation (SSF) by proficient, newly isolated Lactobacillus rhamnosus strain SCJ9. The optimized medium composition revealed by response surface methodology for TSF was 166 g/L LGC hydrolysate and 20 g/L yeast extract (YE), while other medium components were fixed (g/L) as follows: tween80 (2.0), (NH4)2HPO4 (2.0), CH3COONa∙3H2O (6.0), (NH4)2HC6H5O7 (2.0), MgSO4∙7H2O (0.5), and MnSO4∙H2O (0.3). Based on the optimization conditions, the maximum experimental L-lactic acid of 134.6 g/L was achieved at 60 h fermentation time with a production efficiency of 89.73%, 0.95 g/g yield and 2.24 g/L/h productivity. In contrast, L-lactic acid production by SSF under optimized concentrations of thermostable-α-amylase (AA) and glucoamylase (GA) gave maximum L-lactic acid of 125.79 g/L at only 36 h fermentation time which calculated to the production efficiency, yield and productivity of 83.86%, 0.93 g/g and 3.49 g/L/h, respectively. The L-lactic acid production obtained from SSF was significantly improved when compared to TSF based on lower enzyme loading usage, shorter hydrolysis time and increase in production efficiency and productivity. Furthermore, there were no significant differences in the production by SSF between experiments conducted in laboratory bottle and 10-L fermenter. The results indicated the success of up-scaling for L-lactic acid production by SSF which could be developed for a further pilot-scale production of L-lactic acid.

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