Life Cycle Analysis of Integrated Production of Ferulic Acid, 2,3 Butanediol and Microbial Plant Biostimulants from Lignocellulosic Biomass by a Two-Step Cascading Process

A small scale biorefinery process, which includes two main steps: (1) biomass pre-treatment with natural deep eutectic solvents (NADES) and feruloyl-esterase (FAE); and (2) one pot production of a versatile chemical, 2,3 butanediol (2,3-BD), from NADES and FAE pre-treated lignocellulose biomass, by simultaneous saccharification and fermentation (SSF), performed by a plant biostimulant microbial consortia, was developed. [...]

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Screening of Natural Deep Eutectic Solvents for Green Synthesis of 2-methyl-3-substituted Quinazolinones and Microwave-Assisted Synthesis of 3-aryl Quinazolinones in Ethanol

Croatica Chemica Acta ◽

10.5562/cca3597 ◽

2020 ◽

Vol 92 (4) ◽

pp. 511-517

Author(s):

Mario Komar ◽

Maja Molnar ◽

Anastazija Konjarević

Keyword(s):

Green Synthesis ◽

Anthranilic Acid ◽

Deep Eutectic Solvents ◽

13C Nmr Spectroscopy ◽

Microwave Assisted Synthesis ◽

One Pot ◽

1H And 13C Nmr ◽

Microwave Assisted ◽

Natural Deep Eutectic Solvents ◽

Quinazolinone Derivatives

In this study, two fast and efficient protocols for green synthesis of 3-substituted quinazolinones were perfomed. A synthesis of 2-methyl-3-substituted quinazolinones was performed in natural deep eutectic solvents, while 3-aryl quinazolinones were obtained by using microwave assisted synthesis. Benzoxazinone, which was used as an intermediate in the synthesis of 2-methyl-3-substituted quinazolinones, was prepared conventionally from anthranilic acid and acetic anhydride. In order to find the most appropriate synthetic path, twenty natural deep eutectic solvents were applied as a solvent in these syntheses. Choline chloride:urea (1 : 2) was found to be the most efficient solvent and was further used in the synthesis of 2-methyl quinazolinone derivatives (2–12). 3-Aryl quinazolinones (13–17), on the other hand, were synthesized in one-pot microwave-assisted reaction of anthranilic acid, different amines and trimethyl orthoformate. All compounds were synthesized in good to excellent yields, characterized by LC-MS/MS spectrometry and 1H- and 13C-NMR spectroscopy.

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Extrusion-cooking of cassava starch as a pre-treatment for its simultaneous saccharification and fermentation for ethanol production

Acta Alimentaria ◽

10.1556/aalim.32.2003.3.2 ◽

2003 ◽

Vol 32 (3) ◽

pp. 219-235 ◽

Cited By ~ 6

Author(s):

Y.K. Chang ◽

A.A. El-Dash

Keyword(s):

Ethanol Production ◽

Simultaneous Saccharification And Fermentation ◽

Cassava Starch ◽

Extrusion Cooking ◽

Pre Treatment ◽

Simultaneous Saccharification

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One-pot simultaneous production and sustainable purification of fibrinolytic protease from Bacillus cereus using natural deep eutectic solvents

Scientific Reports ◽

10.1038/s41598-020-70414-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Senthil Kumar Rathnasamy ◽

Aadhavan Durai ◽

A. A. Vigneshkumar ◽

C. Purushothaman ◽

Devi Sri Rajendran ◽

...

Keyword(s):

Bacillus Cereus ◽

Deep Eutectic Solvents ◽

One Pot ◽

Simultaneous Production ◽

Natural Deep Eutectic Solvents

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One-pot simultaneous saccharification and fermentation: A preliminary study of a novel configuration for cellulosic ethanol production

Bioresource Technology ◽

10.1016/j.biortech.2014.02.130 ◽

2014 ◽

Vol 161 ◽

pp. 171-178 ◽

Cited By ~ 16

Author(s):

Jingbo Li ◽

Jianghai Lin ◽

Pengfei Zhou ◽

Kejing Wu ◽

Hongmei Liu ◽

...

Keyword(s):

Ethanol Production ◽

Cellulosic Ethanol ◽

Simultaneous Saccharification And Fermentation ◽

One Pot ◽

Preliminary Study ◽

Simultaneous Saccharification

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One-pot preparation of quercetin using natural deep eutectic solvents

Process Biochemistry ◽

10.1016/j.procbio.2019.10.019 ◽

2020 ◽

Vol 89 ◽

pp. 193-198 ◽

Cited By ~ 4

Author(s):

Yuan-Yuan Zang ◽

Xi Yang ◽

Zhi-Gang Chen ◽

Tao Wu

Keyword(s):

Deep Eutectic Solvents ◽

One Pot ◽

Natural Deep Eutectic Solvents

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Pretreatment of Starch-Free Sugar Palm Trunk (Arenga pinnata) to Enhance Saccharification in Bioethanol Production

MATEC Web of Conferences ◽

10.1051/matecconf/201815601003 ◽

2018 ◽

Vol 156 ◽

pp. 01003 ◽

Cited By ~ 1

Author(s):

Kusmiyati ◽

Duwi Maryanto ◽

Ringga Sonifa ◽

Sabda Aji Kurniawan ◽

H. Hadiyanto

Keyword(s):

Simultaneous Saccharification And Fermentation ◽

Lignin Content ◽

Reducing Sugar ◽

Free Sugar ◽

Cellulose Content ◽

Lignocellulosic Materials ◽

Fermentable Sugar ◽

Fermentation Processes ◽

Pre Treatment ◽

Simultaneous Saccharification

Starch-Free Sugar Palm Trunk (Arenga pinnata) can be utilized to produce bioethanol because of their high lignocellulosic contents. Production of bioethanol from lignocellulosic materials consist of pre-treatment, saccharification and fermentation processes. In this work, conversion of starch-free sugar palm trunk (Arenga pinnata) to fermentable sugar and bioethanol was carried out through g pretreatment, saccharification and fermentation processes. The pretreatment was carried out by addition of 1% (v/v) HNO3 and NH4OH for 30 min and 60 min, respectively. The saccharification was carried out at enzyme celullase loadings of 10 and 20 FPU/g and substrate loadings of 10 and 20 g for NH4OH pretreated samples. Fermentation was carried out using two methods i.e. separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) techniques. The results showed that pretreatment using NH4OH was more effective than HNO3 for 60 minutes. IFurthermore, the results also presented the reduction of the lignin content of 9.44% and the increase of cellulose content to 18.56% for 1% (v/v) NH4OH 60 min of pretreatment. The increase of enzyme cellulase (20 FPU/g substrate) and substrate loading (20 g) could produce more reducing sugar (17.423 g/L and 19.233 g/L) than that at 10 FPU/g substrate and 10 g substrate (11.423 g/L and 17.423 g/L), respectively. The comparison of SHF and SSF showed that SHF process yielded higher ethanol (8.11 g/L) as compared to SSF (3.95 g/L) and nontreatment process (0.507 g/L) for 72 h..

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Mutagenesis of novel clostridial fusants for enhanced green biobutanol production using renewable and sustainable feedstock

10.32920/ryerson.14668329.v1 ◽

2021 ◽

Author(s):

Pallavi Roy

Keyword(s):

Wheat Straw ◽

Glucose Concentration ◽

Maximum Concentration ◽

Algal Biomass ◽

Simultaneous Saccharification And Fermentation ◽

Total Sugar ◽

Production Potential ◽

Total Sugar Concentration ◽

Pre Treatment ◽

Simultaneous Saccharification

Biobutanol was produced in the present work through Simultaneous Saccharification and Fermentation (SSF) of cellulosic feedstock (i.e. wheat straw (WS)) and algal biomass. Novel Clostridial fused strains developed earlier underwent mutagenesis for strain enhancement using UV and chemical mutagen (ethyl methanesulphonate). Results for mutated strains showed higher biobutanol production of 14.6 g/L, with total acetone, biobutanol and ethanol (ABE) yield of 0.6 g/g. Moreover, mutated strains showed tolerance to biobutanol toxicity at 15g/L; ~15% increase over literature values. Algal biomass was pre-treated using different thermal, chemical and enzymatic pre-treatments to define its biobutanol production potential compared to WS. A total sugar concentration of 26.4 g/L and glucose concentration of 12.48 g/L was obtained with enzymatic pre-treatment. Biobutanol production through SSF of algal biomass showed maximum concentration of biobutanol of 7.52 g/L with a total ABE yield of 0.48 g/g.

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Prosopis juliflora Bark - An Alternate Feedstock in the Production of Bioethanol using Thermo Tolerant Yeast Kluyveromyces marxianus

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2528 ◽

2017 ◽

Vol 14 (3) ◽

pp. 945-951

Author(s):

S. Sivarathnakumar ◽

G. Baskar ◽

B. Bharathiraja ◽

R. Praveen Kumar ◽

S. Chozhavendhan ◽

...

Keyword(s):

Kluyveromyces Marxianus ◽

Reducing Sugars ◽

Simultaneous Saccharification And Fermentation ◽

Prosopis Juliflora ◽

Lignocellulosic Material ◽

Alternative Source ◽

Inoculum Concentration ◽

Perennial Plant ◽

Pre Treatment ◽

Simultaneous Saccharification

ABSTRACT: Prosopis juliflora, a widely available perennial plant can be an alternative source to sugar-containing feedstock, which can be considered as a prospective lignocellulosic material for bioethanol production. In the present study, bark of Prosopis juliflora was subjected to hydrothermal coupled with nitric acid pre-treatment (3%(v/v)) followed by sonication. The composition of cellulose, hemicellulose, lignin, reducing sugars and inhibitors at each stage of pre-treatment were analysed. Further, delignified lignocellulosic biomass was subjected to Simultaneous Saccharification and Fermentation (SSF) studies using Kluyveromyces marxianus (MTCC 1389) and commercial cellulase enzyme. The effect of operating parameters such as pH, temperature, substrate concentration and inoculum concentration were investigated and found to be 4.9, 41oC, 3% v/v and 5% w/v respectively. The maximum bioethanol concentration achieved by fermentation of woody stem Prosopis juliflora using the yeast was found to be 21.45g/l.

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