Bioethanol production from wheat straw by the thermotolerant yeast Kluyveromyces marxianus CECT 10875 in a simultaneous saccharification and fermentation fed-batch process

Fuel ◽  
2009 ◽  
Vol 88 (11) ◽  
pp. 2142-2147 ◽  
Author(s):  
E. Tomás-Pejó ◽  
J.M. Oliva ◽  
A. González ◽  
I. Ballesteros ◽  
M. Ballesteros
Keyword(s):  
Wheat Straw ◽  
Kluyveromyces Marxianus ◽  
Simultaneous Saccharification And Fermentation ◽  
Batch Process ◽  
Thermotolerant Yeast ◽  
Bioethanol Production ◽  
Fed Batch ◽  
Simultaneous Saccharification

Optimization of Process Parameters for Ethanol Production from Alkaline-Pretreated Wheat Straw by Fed-Batch Simultaneous Saccharification and Fermentation

2011 ◽  
Vol 236-238 ◽  
pp. 922-925
Author(s):  
Chun Chang ◽  
Gui Zhuan Xu ◽  
Xiao Jian Ma
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Raw Materials ◽  
Feeding Strategy ◽  
Tween 20 ◽  
Fed Batch ◽  
Pretreated Wheat Straw ◽  
Simultaneous Saccharification

The alkaline-pretreated wheat straw was used as raw materials, and the fed-batch simultaneous saccharification and fermentation (SSF) process for ethanol production with higher substrate loading (up to 16% (w/v)) was investigated. Firstly, the Fed-batch SSF with different feeding strategy was optimized. The highest ethanol concentration reached to 37.9 g/L with an initial substrate loading of 10% (w/v), and equal substrate addition (3%) at 8h and 16h respectively. Then the effects of Tween-20 were also investigated. The results showed that Tween-20 addition with 2.0 g/L can improved the ethanol production. Furthermore, a nonisothermal fed-batch SSF was proposed, and the ethanol concentration of 41.5 g/L, corresponds to 81.0 % of the theoretical yield was achieved within 72 h.

scholarly journals Co-Production of Isobutanol and Ethanol from Prairie Grain Starch Using Engineered Saccharomyces cerevisiae

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 150
Author(s):  
Xiaodong Liu ◽  
Ebele Unaegbunam ◽  
David T. Stuart
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification And Fermentation ◽  
Batch Process ◽  
Energy Crop ◽  
Combustion Engines ◽  
Fed Batch ◽  
Fuel Distribution ◽  
Grain Starch ◽  
Engineered Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification

Isobutanol is an important and valuable platform chemical and an appealing biofuel that is compatible with contemporary combustion engines and existing fuel distribution infrastructure. The present study aimed to compare the potential of triticale, wheat and barley starch as feedstock for isobutanol production using an engineered strain of Saccharomyces cerevisiae. A simultaneous saccharification and fermentation (SSF) approach showed that all three starches were viable feedstock for co-production of isobutanol and ethanol and could produce titres similar to that produced using purified sugar as feedstock. A fed-batch process using triticale starch yielded 0.006 g isobutanol and 0.28 g ethanol/g starch. Additionally, it is demonstrated that Fusarium graminearum infected grain starch contaminated with mycotoxin can be used as an effective feedstock for isobutanol and ethanol co-production. These findings demonstrate the potential for triticale as a purpose grown energy crop and show that mycotoxin-contaminated grain starch can be used as feedstock for isobutanol biosynthesis, thus adding value to a grain that would otherwise be of limited use.

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