Insulin Regulation of Phosphoenolpyruvate Carboxykinase-C Gene Transcription: The Role of Sterol Regulatory Element-Binding Protein 1c

2007 ◽  
Vol 65 (6) ◽  
pp. 47-56 ◽  
Author(s):  
Kaushik Chakravarty ◽  
Richard W. Hanson
2001 ◽  
Vol 276 (37) ◽  
pp. 34816-34823 ◽  
Author(s):  
Kaushik Chakravarty ◽  
Patrick Leahy ◽  
Dominique Becard ◽  
Parvin Hakimi ◽  
Marc Foretz ◽  
...  

Life Sciences ◽  
2016 ◽  
Vol 152 ◽  
pp. 178-179
Author(s):  
Dennys E. Cintra ◽  
Eduardo R. Ropelle ◽  
Marcelo F. Vitto ◽  
Thais F. Luciano ◽  
Daniela R. Souza ◽  
...  

2007 ◽  
Vol 17 (1) ◽  
pp. 62-65 ◽  
Author(s):  
Marek Szolkiewicz ◽  
Michal Chmielewski ◽  
Anna Nogalska ◽  
Ewa Stelmanska ◽  
Julian Swierczynski ◽  
...  

2007 ◽  
Vol 292 (1) ◽  
pp. G369-G376 ◽  
Author(s):  
Waddah A. Alrefai ◽  
Fadi Annaba ◽  
Zaheer Sarwar ◽  
Alka Dwivedi ◽  
Seema Saksena ◽  
...  

Niemann-Pick C1-like 1 (NPC1L1) is an essential intestinal component of cholesterol absorption. However, little is known about the molecular regulation of intestinal NPC1L1 expression and promoter activity. We demonstrated that human NPC1L1 mRNA expression was significantly decreased by 25-hydroxycholesterol but increased in response to cellular cholesterol depletion achieved by incubation with Mevinolin (an inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase) in human intestinal Caco-2 cells. We also showed that a −1741/+56 fragment of the NPC1L1 gene demonstrated high promoter activity in Caco-2 cells that was reduced by 25-hydroxycholesterol and stimulated by cholesterol depletion. Interestingly, we showed that the NPC1L1 promoter is remarkably transactivated by the overexpression of sterol regulatory element (SRE) binding protein (SREBP)-2, suggesting its involvement in the sterol-induced alteration in NPC1L1 promoter activity. Finally, we identified two putative SREs in the human NPC1L1 promoter and established their essential roles in mediating the effects of cholesterol on promoter activity. Our study demonstrated the modulation of human NPC1L1 expression and promoter activity by cholesterol in a SREBP-2-dependent mechanism.


FEBS Letters ◽  
2005 ◽  
Vol 580 (2) ◽  
pp. 410-414 ◽  
Author(s):  
Claudine Gregori ◽  
Isabelle Guillet-Deniau ◽  
Jean Girard ◽  
Jean-François Decaux ◽  
Anne-Lise Pichard

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