scholarly journals Egg diapause in Alydus calcaratus (L.) (Heteroptera : Alydidae) : Photoperiodic induction under natural temperature conditions

1999 ◽  
Vol 34 (4) ◽  
pp. 469-473 ◽  
Author(s):  
Dmitry L. Musolin ◽  
Andrey Maysov ◽  
Aida H. Saulich
Nematology ◽  
2010 ◽  
Vol 12 (2) ◽  
pp. 193-200 ◽  
Author(s):  
Elif Sahin ◽  
Julie M. Nicol ◽  
I. Halil Elekcioglu ◽  
Roger Rivoal

Abstract The effect of different temperatures under both in vitro and in vivo conditions on the hatching behaviour of second-stage juveniles (J2) of the cereal cyst nematode, Heterodera filipjevi, was studied. Cumulative percent hatching was affected significantly by temperature after 290 days of incubation. Hatching was significantly greater at lower temperatures (5, 10 and 15°C) compared with that at the higher temperatures of 20 and 25°C, ranging between 75 and 94% vs 19 and 22%, respectively. The highest cumulative hatch of 94% was obtained at a constant temperature of 15°C at 290 days. However, the lowest cumulative hatch of 33% was obtained after initial exposure to 5°C followed by transfer to 25°C at day 290. In general, incubating the cysts at lower initial temperatures of 10 or 15°C for 58 days gave the highest initial hatching rates 1 week after exposure to the final temperatures. Under natural temperature conditions in the field, J2 emergence started at 17°C in October and continued until the end of April in the temperature range of 2-17°C. A total hatch of 94% was recorded under field temperature conditions over the course of 1 year. Hatch of most J2 occurred in two peaks; the first in October and the second in February, and, accordingly, the greatest invasion by H. filipjevi is most likely to occur just after these two peaks. Heterodera filipjevi does not seem to have a diapause and could hatch anytime when wheat plants are available.


2009 ◽  
Vol 48 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Hea-Son Bang ◽  
Myung-Hyun Kim ◽  
Myung-Pyo Jung ◽  
Min-Su Han ◽  
Young-Eun Na ◽  
...  

1993 ◽  
Vol 87 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Ivana Machackova ◽  
Jan Krekule ◽  
Josef Eder ◽  
Frideta Seidlova ◽  
Miroslav Strnad

2019 ◽  
Vol 15 (3) ◽  
Author(s):  
Trismillah

Cavendish banana peel can be used as a substitute for the expensive xylan, while molasses than as a source of carbon as well as nitrogen, minerals and nutrients needed for the growth of microbes that can produce the enzyme. Xylanase produced from Bacillus stearothermopillus DSM 22, using media cavendish banana peels with the addition of molasses 1%, 2%, and 3%. Fermentation is done in a shaker incubator at 550C temperature conditions, initial pH 8, and 250 rpm agitation. The result showed the highest enzyme activity of 4,14 ± 0,16 U/mL min., on the addition 2% molasses after 24 hours. Further fermentation carried out in the fermenter working volume of 3.5 liters, with the condition of temperature 550C, pH 8, aeration 1 vvm, agitation 250 rpm, the highest spesific enzyme of activity of 51,62 ± 0,16 U/mg after 24 hours. Partial purification of xylanase enzyme fermentation is done with the results of microfiltration, ultrafiltration, ammonium sulfate (0-80%) and dialysis. There is an increase in the purity of the enzyme at each stage of purification, the highest purity on dialysis 3.23 times of crude enzymes.Kulit buah pisang kapendis dapat digunakan sebagai pengganti xilan yang harganya mahal, sementara molases selain sebagai sumber karbon serta nitrogen, mineral dan nutrisi dibutuhkan untuk pertumbuhan mikroba yang dapat menghasilkan enzim. Xilanase yang dihasilkan dari Bacillus stearothermopillus DSM 22, menggunakan media kulit pisang kapendis dengan penambahan molase 1%, 2%, dan 3%. Fermentasi dilakukan dalam shaker inkubator pada temperatur 550C, pH awal 8, dan agitasi 250 rpm. Hasilnya menunjukkan aktivitas enzim tertinggi 4,14 ± 0,16 U/mL min., pada penambahan 2% molases setelah 24 jam. Selanjutnya fermentasi dilakukan di dalam fermentor, volume kerja dari 3,5 liter, dengan kondisi temperatur 550C, pH 8, aeration 1 vvm, agitasi 250 rpm, aktivitas spesifik tertinggi 51,62 ± 0,16 U/mg setelah 24 jam. Pemurnian parsial fermentasi enzim xilanase dilakukan dengan hasil mikrofiltrasi, ultrafiltrasi, amonium sulfat (0-80%) dan dialisis. Ada peningkatan kemurnian enzim pada setiap tahap pemurnian, kemurnian tertinggi pada dialisis 3,23 kali dari enzim kasar.Keywords: Xylanase, B. stearothermophillus DSM 22, Cavendish banana peel, molasses, enzyme activity


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