A cytokinin-containing seaweed extract, Nitrozyme™ (Nitrozyme1), was tested under field conditions over 4 yr for its effects on the growth and yield of barley (Hordeum vulgare L.). In 1986 and 1987, Nitrozyme did not increase yield and had no beneficial effects on growth of Harrington and Bonanza barley. In 1988 and 1989, the effects of Nitrozyme on barley (cv. Leduc) were compared against those of kinetin, a synthetic cytokinin. Neither Nitrozyme nor kinetin had an effect on growth or yield.Key words: Nitrozyme, seaweed extracts, cytokinins, kinetin, plant growth regulator, Hordeum vulgare L.