scholarly journals Taxonomy confirmation of Puntius cf. binotatus from Gunung Tujuh Lake, Jambi, Indonesia based on Cytochrome Oxidase-I (COI) gene

2018 ◽  
Vol 20 (1) ◽  
pp. 54-60
Author(s):  
DEWI IMELDA ROESMA ◽  
DJONG HON TJONG ◽  
WILA KARLINA ◽  
DYTA RABBANI AIDIL
Keyword(s):  
Cytochrome Oxidase ◽  
Black Spot ◽  
Taxonomic Status ◽  
Molecular Study ◽  
Morphological Characters ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Phenotypic Characters ◽  
Subspecies Level ◽  
West Sumatra

Roesma D. I, Tjong D. H, Karlina W, Aidil D. R. 2019. Taxonomy confirmation of Puntius cf. binotatus from Gunung Tujuh Lake based on Cytochrome Oxidase-I (COI) gene. Biodiversitas 20: 54-60. The population of Puntius cf. binotatus Gunung Tujuh Lake in considered as an isolated population which found in the 20-30 meters depth under the water Gunung Tujuh Lake, Sumatra Island. The species often exhibit different phenotypic characters and may genetic characters may also different due to their response to habitat/ecological conditions. Previous study on P. binotatus from several locations in West Sumatra showed the complexity of the genetic and morphological characters. A molecular study using COI gene of P. cf. binotatus from Gunung Tujuh Lake in Sumatera has been conducted to determine the taxonomic status of the fish. The distinct morphological characters of Puntius cf. binotatus is one black spot in the middle of the caudal and the absent of black spot on the base of the anterior dorsal ray. The analysis on COI gene showed that the range of sequence divergences between P. cf. binotatus Gunung Tujuh Lake and other Sumatra P. cf. binotatus is 3.1-7.6% and 4.1% to Barbodes banksi. This value represents the differences at the subspecies level of Barbodes banksi. Therefore Barbodes banksi gunungtujuh is proposed as an appropriate name to P. cf. binotatus Gunung Tujuh Lake.

scholarly journals Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
DEWI IMELDA ROESMA ◽  
DJONG HON TJONG ◽  
DYTA RABBANI AIDIL
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome Oxidase ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Phylogenetic Study ◽  
Mitochondrial Cytochrome ◽  
Base Pairs ◽  
Low Genetic Diversity ◽  
West Sumatra ◽  
Mitochondrial Cytochrome Oxidase

Abstract. Roesma DI, Tjong DH, Aidil DR. 2020. Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene. Biodiversitas 21: 43-48. The transparent gobies fish found in three lakes in Sumatra island is known as Rinuak fish (in Maninjau Lake and Singkarak Lake, West Sumatra, Indonesia) or Badar fish (in Siais Lake, North Sumatra, Indonesia), and are morphologically very similar to the Gobiopterus brachypterus. The phylogenetic study was carried out by analyzing 619 base pairs of the mitochondrial DNA cytochrome oxidase subunit I (COI) gene in 12 fish individuals from the three lakes. Rinuak and Badar fish in three populations have four haplotypes. The sequence divergences in and between populations are very low (0.0-0.5%). This value indicates that Rinuak and Badar fish are the same species with low genetic diversity. The phylogenetic tree illustrates that this fish belongs to the group of Gobiidae and a sister taxon from G. brachypterus.

Current issues in species identification for forensic science and the validity of using the cytochrome oxidase I (COI) gene

2010 ◽  
Vol 6 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Linzi Wilson-Wilde ◽  
Janette Norman ◽  
James Robertson ◽  
Stephen Sarre ◽  
Arthur Georges
Keyword(s):  
Forensic Science ◽  
Cytochrome Oxidase ◽  
Species Identification ◽  
Cytochrome Oxidase I ◽  
Coi Gene

Mitochondrial DNA sequence evidence supporting the recognition of a new North Atlantic Pseudostichopus species (Echinodermata: Holothuroidea)

2004 ◽  
Vol 84 (5) ◽  
pp. 1077-1084 ◽  
Author(s):  
Francisco A. Solís-Marín ◽  
David S.M. Billett ◽  
Joanne Preston ◽  
Alex D. Rogers
Keyword(s):  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Distinct Species ◽  
Molecular Study ◽  
Morphological Characters ◽  
Coi Gene ◽  
Species Status ◽  
Separate Species ◽  
The Family ◽  
A New Species

A new species of the synallactid sea cucumber genus Pseudostichopus is described, P. aemulatus sp. nov., based on genetic (DNA sequences of the mitochondrial gene Cytochrome Oxidase I [COI] gene) and morphological characters. A comparative molecular study with two other species of the same genus (P. villosus and P. mollis) and from a different family (Isostichopus fuscus) was carried out in order to clarify its taxonomic identity. The nucleotide distance between P. aemulatus sp. nov. and P. villosus and P. mollis is sufficient to support distinct species status. The estimated difference in the number of amino acids, coded for by a partially sequenced COI gene, within the species of the family Synallactidae ranged from 4 to 18. The phylogenetic analysis clearly supports separate species status of these sympatric morphotypes, as indicated by the morphological analysis.

Five new species of Myrsidea Waterston (Phthiraptera: Menoponidae) from tanagers (Passeriformes: Thraupidae) in Panama

Zootaxa ◽  
2009 ◽  
Vol 2200 (1) ◽  
pp. 61-68 ◽  
Author(s):  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON
Keyword(s):  
New Species ◽  
Cytochrome Oxidase ◽  
Species Group ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Avian Family ◽  
Mitochondrial Cytochrome Oxidase

There are 23 species of Myrsidea recognized from passerine thraupid hosts. Five new species parasitic on members of this avian family are described. They and their type hosts are Myrsidea rozsai ex the Thick-billed Euphonia, Euphonia laniirostris d'Orbigny & Lafresnaye, M. cruickshanki ex the Carmiol's Tanager, Chlorothraupis carmioli (Lawrence), M. patersoni ex the Grey-headed Tanager, Eucometis penicillata (Spix), M. pagei ex the Crimson-backed Tanager, Ramphocelus dimidiatus Lafresnaye, and M. valimi ex the Tawny-capped Euphonia, Euphonia anneae Cassin. The first species is placed in the bonariensis species group, while the remaining four are placed in the fusca species group. Sequences of a portion of the mitochondrial cytochrome oxidase I (COI) gene were compared to other species of Myrsidea and were highly divergent.

The Madagascan genus Dolurgocleptes Schedl (Coleoptera: Curculionidae, Scolytinae): description of a new species and transfer to the tribe Polygraphini

Zootaxa ◽  
2009 ◽  
Vol 2014 (1) ◽  
pp. 41-50 ◽  
Author(s):  
BJARTE H. JORDAL
Keyword(s):  
New Species ◽  
Cytochrome Oxidase ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Cytochrome Oxidase I ◽  
Elongation Factor 1Α ◽  
North Eastern ◽  
A New Species

A new species of the Malagasy genus Dolurgocleptes Schedl, 1965 is described and illustrated. This is the second species known for the genus, which is restricted to the montane rainforests of north-eastern Madagascar. Dolurgocleptes is transferred from the tribe Dryocoetini to Polygraphini and placed near Polygraphus Erichson, based on examination of internal and external morphological characters and molecular data from Elongation Factor-1α and Cytochrome Oxidase I.

The Comparative Molecular Study between Bombycidae and Saturniidae Based on mtDNA RFLP and Cytochrome Oxidase I Gene Sequences: Implication for Molecular Evolution

1999 ◽  
Vol 54 (7-8) ◽  
pp. 587-594 ◽  
Author(s):  
Jae-Sam Hwang ◽  
Jin-Sung Lee ◽  
Tae-Won Goo ◽  
Hyun-Ah Kang ◽  
Hae-Ryong Sohn ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Study ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Confidence Limits ◽  
Sequence Identity ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene ◽  
Mtdna Rflp

Abstract Bombycidae, Saturniidae, mtDNA RFLP, Cytochrome Oxidase I Gene The phylogenetic relationships between Bombyx mori and Bombyx mandarina species of Bombycidae, and Antheraea yamamai and Antheraea pernyi species of Saturniidae were investigated based on mtDNA RFLP and cytochrome oxidase I gene. The sizes of the mtDNA of all the species were estimated at approximately 16 kbp ± 500 bp by total length of all the restricted fragments and no variation in size was recognized. Of the fourteen different restriction endonucleases used, BamHl, Hindlll, Pstl, EcoRl and Xbal showed RFLP. Among these, only Hindlll showed RFLP between B. mori and B. mandarina. A comparative analysis of sequences was also conducted with the mitochondrial cytochrome oxidase I genes of each species. The results indicated that B. mori shared a 97% , 85% and 87% sequence identity with B. mandarina, A. yamamai and A. pernyi, respectively. B. mandarina shared a 87% and 88% sequence identity with A. yamamai and A. penyi, respectively. A. yamamai shared 92% sequence identity with A. pernyi. The results of the phylogenetic analysis exhib­ited monophyly and confidence limits of more than 99% in all trees for both Bombycidae and Saturniidae.

scholarly journals Pengujian Dua Pasang Primer Universal Untuk Amplifikasi Gen Cytochrome Oxidase I Copepoda

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 93
Author(s):  
Thalita C. P. Sumampow
Keyword(s):  
Cytochrome Oxidase ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Universal Primer ◽  
Cytochrome Oxidase I Gene ◽  
I Gene

Copepoda merupakan zooplankton kaya manfaat dengan diversitas yang sangat tinggi dan terdiri dari banyak spesies kriptik. Identifikasi cepat, akurat, dan hemat dapat dilakukan dengan menggunakan teknik DNA Barcoding. Kesuksesan teknik tersebut sangat dipengaruhi oleh penggunaan primer yang tepat. Tujuan penelitian ini adalah untuk menguji kemampuan dua pasang primer universal, yakni LCO1490-HCO2198 dan FF2d-FR1d, mengamplifikasi gen COI Copepoda. Dalam penelitian ini, pasangan primer LCO1490-HCO2198 tidak berhasil mengamplifikasi gen target. Sekuens-sekuens hasil amplifikasi menggunakan pasangan primer FF2d-FR1d diidentifikasi melalui BLAST. Hasil yang diperoleh menunjukan bahwa sekuens-sekuens tersebut memiliki persentase kemiripan sebesar 92% dengan bakteri Pandoraea pnomenusa. Melalui hasil yang didapatkan disimpulkan bahwa kedua pasangan primer universal LCO1490-HCO2198 dan FF2d-FR1d tidak cukup spesifik untuk amplifikasi gen cytochrome oxidase I Copepoda.Copepoda is a very beneficial and highly diverse zooplankton with many cryptic species. A fast, reliable, and affordable identification can be done through DNA Barcoding. The success of this technique is affected by the usage of correct primers. The aim of this research was to test the ability of two universal primer pairs, which were LCO1490-HCO2198 and FF2d-FR1d, amplifying COI gene of Copepoda. In this research, LCO1490-HCO2198 primer pairs weren’t able to amplify COI gene of Copepoda. Sequences which were successfully amplified using FF2d-FR1d primer pairs were identified through BLAST. The result shows that the sequences are 92% similar to bacteria named Pandoraea pnomenusa. It can be concluded that both primer pairs are not specific enough to amplify cytochrome oxidase I gene of Copepoda.

scholarly journals Integrative Taxonomy of Two Peruvian Strains of Brachionus plicatilis Complex with Potential in Aquaculture

Diversity ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 671
Author(s):  
Pedro Pablo Alonso Sánchez-Dávila ◽  
Giovanna Sotil ◽  
Araceli Adabache-Ortiz ◽  
Deivis Cueva ◽  
Marcelo Silva-Briano
Keyword(s):  
Egg Production ◽  
Brachionus Plicatilis ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Reproductive Age ◽  
Coi Gene ◽  
Phylogenetic Position ◽  
Mtdna Coi ◽  
The Relationship

Two Peruvian strains of the genus Brachionus were isolated from impacted coastal wetlands. With an integrative taxonomic view, we described their taxonomic status, morphological characters, productive parameters, and phylogenetic position. In the case of both strains, the relationship between biometrics and productive parameters obtained with Principal Components Analysis indicated that the lorica length was associated with longevity, progeny, egg production, and reproductive age, while the lorica width and aperture were associated with the maximum number of eggs carried. Maximum Likelihood and Bayesian Inference analysis carried out with mtDNA COI gene and rDNA ITS1 region showed that both strains were clustered in two clades with distinct phylogenetic positioning from what is currently known for Brachionus plicatilis s.l. One of the strains, Z010-VL, is proposed to be a subspecies of L4 (B. paranguensis), and the other strain, Z018-SD, is proposed as a sub species of SM2 (B. koreanus). In addition, 33 and 31 aquaculture production lineages are proposed, delimited by COI and concatenated COI+ITS1 sequences, respectively. Finally, this study provides new tools that enhance the traceability of the origin of each sub-species throughout the world.

scholarly journals The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool

2018 ◽  
Vol 19 (3) ◽  
pp. 997-1003
Author(s):  
R. SUSANTI ◽  
RETNO SRI ISWARI ◽  
FIDIA FIBRIANA ◽  
INDRIAWATI INDRIAWATI
Keyword(s):  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Gene Sequence ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Central Java ◽  
Synonym Substitution ◽  
Haplotype A

Susanti R, Iswari RS, Fibriana F, Indriawati. 2018. The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool. Biodiversitas 19: 997-1003. The local duck DNA barcoding is still rarely conducted in Indonesia while DNA barcoding is extensively used as a tool of species identification and delineation tool. This study aimed to analyze the sequence and patterns of Central Javanese ducks mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Feather samples of seven breeds of native duck were collected from traditional husbandries in Central Java. The samples were employed for DNA extraction and COI gene amplification. Five haplotypes were obtained from 35 samples, i.e., haplotype A, B, C, D, and E. Also, 9 variable sites with synonym substitution was detected in four nucleotides number 55, 61, 100, and 109; whereas five synonym substitutions were identified in the nucleotides number 36, 48, 51, 66, and 756. In conclusion, this study annotates that COI mtDNA gene is essential for local ducks barcoding system.

scholarly journals MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE

2018 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
I Putu Sudiarta ◽  
Dwi Martiningsia ◽  
I Nyoman Wijaya
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Identification ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Morphological Identification ◽  
Mitochondrial Cytochrome ◽  
Accurate Identification ◽  
Mitochondrial Cytochrome Oxidase

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.

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