Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes

Abstract. Maharsiwi W, Astuti RI, Meryandini A, Wahyudi AT. 2020. Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes. Biodiversitas 21: 975-981. Exploration of new enzymes from an extreme environment is important to improve industrial efficiency. This study aimed to get sponge-associated bacteria from Seribu Island with the capability to produce cellulase and laccase. These enzyme activities were indicated by the clear zones on CMC medium for cellulase and the reddish-brown zone on Guaiacol medium for laccase. About 100 of sponge-associated bacteria have been isolated from 5 marine sponges used SWC and NA modified media. As screened, one isolate (AGN89) could produce both enzymes and 11 isolates could produce cellulase. Quantitative analysis was performed using the DNS method and obtained the activities of 4 best cellulolytic isolates ranged from 0.04-0.06 UmL-1 and 0.70-1.18 UmL-1 in enzyme and specific activities, respectively. Gene-based determination for the isolate producing laccase resulted in a ±1100 bp amplicon fragment which identified as multicopper oxidase family protein. Based on the 16S-rRNA gene, AGN89 and these 4 cellulolytic isolates were identified as Pseudomonas luteola strain NBRC 103146, Bacillus aerius strain 24k, Pseudomonas aeruginosa strain DSM 50071, Mycobacterium maritypicum strain DSM 20578, and Brachybacterium conglomeratum strain J 1015. This result suggests that the sponge-associated bacteria from Seribu Island could become new enzymes producer for further applications in industry.

Download Full-text

Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths

Marine Drugs ◽

10.3390/md17100578 ◽

2019 ◽

Vol 17 (10) ◽

pp. 578 ◽

Cited By ~ 1

Author(s):

Anak Indraningrat ◽

Sebastian Micheller ◽

Mandy Runderkamp ◽

Ina Sauerland ◽

Leontine Becking ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Marine Sponges ◽

Plate Count ◽

Rrna Gene ◽

Depth Range ◽

Prokaryotic Community ◽

Associated Bacteria ◽

Xestospongia Muta ◽

Sponge Associated Bacteria

Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges Xestospongia muta and Agelas sventres collected from shallow (<30 m), upper mesophotic (30–60 m), and lower mesophotic (60–90 m) reefs. Sponge-associated bacteria were cultivated on six different media, and replicate plates were used to pick individual colonies or to recover the entire biomass. Prokaryotic community analysis was conducted using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 144 bacterial isolates were picked following a colony morphology coding scheme and subsequently identified by 16S rRNA gene sequence analysis. Sponge individuals at each depth-range harboured specific cultivable bacteria that were not retrieved from specimens collected at other depths. However, there were substantial differences in the number of colonies obtained for replicate sponges of the same species. In addition, source of inoculum and cultivation medium had more impact on the cultured prokaryotic community than sample collection depth. This suggests that the “plate count anomaly” is larger than differences in sponge-associated prokaryotic community composition related to depth.

Download Full-text

PRIMARY CHARACTERIZATION OF SPONGE ASSOCIATED BACTERIA OF MARINE SPONGES - HALICHONDRIA GLABRATA, CLIONA LOBATA, SPIRASTRELLA PACHYSPIRA AND THEIR ANTIMICROBIAL PROPERTIES

Journal of Microbiology Biotechnology and Food Sciences ◽

10.15414/jmbfs.2014.4.2.117-121 ◽

2014 ◽

Vol 04 (02) ◽

pp. 117-121 ◽

Cited By ~ 1

Author(s):

Maushmi Kumar ◽

Shaishav Jogani

Keyword(s):

Antimicrobial Properties ◽

Marine Sponges ◽

Associated Bacteria ◽

Sponge Associated Bacteria

Download Full-text

Characterization of symbiotic and associated bacteria from entomopathogenic nematode Heterorhabditis sp. (nematode: Heterorhabditidae) isolated from India

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-020-00343-9 ◽

2020 ◽

Vol 30 (1) ◽

Author(s):

Rashid Pervez ◽

Showkat Ahmad Lone ◽

Sasmita Pattnaik

Keyword(s):

Insect Pests ◽

Entomopathogenic Nematode ◽

Alcaligenes Faecalis ◽

Symbiotic Bacteria ◽

Rrna Gene ◽

Main Body ◽

Associated Bacteria ◽

Molecular Approaches

Abstract Background Entomopathogenic nematodes (EPNs) harboring symbiotic bacteria are one of the safest alternatives to the chemical insecticides for the control of various insect pests. Infective juveniles of EPNs locate a target insect, enter through the openings, and reach the hemocoel, where they release the symbiotic bacteria and the target gets killed by the virulence factors of the bacteria. Photorhabdus with Heterorhabditis spp. are well documented; little is known about the associated bacteria. Main body In this study, we explored the presence of symbiotic and associated bacteria from Heterorhabditis sp. (IISR-EPN 09) and characterized by phenotypic, biochemical, and molecular approaches. Six bacterial isolates, belonging to four different genera, were recovered and identified as follows: Photorhabdus luminescens, one each strain of Providencia vermicola, Pseudomonas entomophila, Alcaligenes aquatilis, and two strains of Alcaligenes faecalis based on the phenotypic, biochemical criteria and the sequencing of 16S rRNA gene. Conclusion P. luminescens is symbiotically associated with Heterorhabditis sp. (IISR-EPN 09), whereas P. vermicola, P. entomophila, A. aquatilis, and A. faecalis are the associated bacteria. Further studies are needed to determine the exact role of the bacterial associates with the Heterorhabditis sp.

Download Full-text

Screening and Characterization of Sponge-Associated Bacteria Producing Bioactive Compounds Anti-Vibrio sp.

American Journal of Biochemistry and Biotechnology ◽

10.3844/ajbbsp.2018.221.229 ◽

2018 ◽

Vol 14 (3) ◽

pp. 221-229 ◽

Cited By ~ 1

Author(s):

Aris Tri Wahyudi ◽

Jepri Agung Priyanto ◽

Wenang Maharsiwi ◽

Rika Indri Astuti

Keyword(s):

Bioactive Compounds ◽

Associated Bacteria ◽

Sponge Associated Bacteria

Download Full-text

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Applied and Environmental Microbiology ◽

10.1128/aem.05285-11 ◽

2011 ◽

Vol 77 (20) ◽

pp. 7207-7216 ◽

Cited By ~ 76

Author(s):

Naomi F. Montalvo ◽

Russell T. Hill

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bacterial Communities ◽

Sponge Species ◽

Rrna Gene ◽

Gene Sequences ◽

Associated Bacteria ◽

Content Type ◽

Key Species ◽

Sponge Associated Bacteria

ABSTRACTThe giant barrel spongesXestospongiamutaandXestospongiatestudinariaare ubiquitous in tropical reefs of the Atlantic and Pacific Oceans, respectively. They are key species in their respective environments and are hosts to diverse assemblages of bacteria. These two closely related sponges from different oceans provide a unique opportunity to examine the evolution of sponge-associated bacterial communities. Mitochondrial cytochrome oxidase subunit I gene sequences fromX.mutaandX.testudinariashowed little divergence between the two species. A detailed analysis of the bacterial communities associated with these sponges, comprising over 900 full-length 16S rRNA gene sequences, revealed remarkable similarity in the bacterial communities of the two species. Both sponge-associated communities include sequences found only in the twoXestospongiaspecies, as well as sequences found also in other sponge species and are dominated by three bacterial groups,Chloroflexi,Acidobacteria, andActinobacteria. While these groups consistently dominate the bacterial communities revealed by 16S rRNA gene-based analysis of sponge-associated bacteria, the depth of sequencing undertaken in this study revealed clades of bacteria specifically associated with each of the twoXestospongiaspecies, and also with the genusXestospongia, that have not been found associated with other sponge species or other ecosystems. This study, comparing the bacterial communities associated with closely related but geographically distant sponge hosts, gives new insight into the intimate relationships between marine sponges and some of their bacterial symbionts.

Download Full-text

Characterization of polybacterial clinical samples using a set of group-specific broad-range primers targeting the 16S rRNA gene followed by DNA sequencing and RipSeq analysis

Journal of Medical Microbiology ◽

10.1099/jmm.0.028373-0 ◽

2011 ◽

Vol 60 (7) ◽

pp. 927-936 ◽

Cited By ~ 26

Author(s):

Øyvind Kommedal ◽

Katrine Lekang ◽

Nina Langeland ◽

Harald G. Wiker

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Dna Sequencing ◽

Clinical Samples ◽

Rrna Gene ◽

The 16S Rrna Gene

Download Full-text

Molecular Characterization of anEndozoicomonas-Like Organism Causing Infection in the King Scallop (Pecten maximusL.)

Applied and Environmental Microbiology ◽

10.1128/aem.00952-17 ◽

2017 ◽

Vol 84 (3) ◽

Cited By ~ 1

Author(s):

Irene Cano ◽

Ronny van Aerle ◽

Stuart Ross ◽

David W. Verner-Jeffreys ◽

Richard K. Paley ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Molecular Characterization ◽

Mass Mortality ◽

Rrna Gene ◽

Gene Sequences ◽

16S Rrna Gene Sequences ◽

Content Type ◽

The 16S Rrna Gene

ABSTRACTOne of the fastest growing fisheries in the UK is the king scallop (Pecten maximusL.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay marine protected area (MPA) in Southwest England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies (IMCs) of bacteria resemblingRickettsia-like organisms (RLOs), often with bacteria released in vascular spaces. Large colonies were associated with cellular and tissue disruption of the gills. Ultrastructural examination confirmed the intracellular location of these organisms in affected epithelial cells. The 16S rRNA gene sequences of the putative IMCs obtained from infected king scallop gill samples, collected from both mortality events, were identical and had a 99.4% identity to 16S rRNA gene sequences obtained from “CandidatusEndonucleobacter bathymodioli” and 95% withEndozoicomonasspecies.In situhybridization assays using 16S rRNA gene probes confirmed the presence of the sequenced IMC gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing, and bioinformatic analysis identified over 1,000 genes with high similarity to protein sequences fromEndozoicomonasspp. (ranging from 77 to 87% identity). Specific PCR assays were developed and applied to screen for the presence of IMC 16S rRNA gene sequences in king scallop gill tissues collected at the Lyme Bay MPA during 2015 and 2016. There was 100% prevalence of the IMCs in these gill tissues, and the 16S rRNA gene sequences identified were identical to the sequence found during the previous mortality event.IMPORTANCEMolluscan mass mortalities associated with IMCs have been reported worldwide for many years; however, apart from histological and ultrastructural characterization, characterization of the etiological agents is limited. In the present work, we provide detailed molecular characterization of anEndozoicomonas-like organism (ELO) associated with an important commercial scallop species.

Download Full-text

Variation in the microbiome of the spider mite Tetranychus truncatus with sex, instar and endosymbiont infection

FEMS Microbiology Ecology ◽

10.1093/femsec/fiaa004 ◽

2020 ◽

Vol 96 (2) ◽

Author(s):

Yu-Xi Zhu ◽

Zhang-Rong Song ◽

Shi-Mei Huo ◽

Kun Yang ◽

Xiao-Yue Hong

Keyword(s):

Spider Mite ◽

Potential Role ◽

Developmental Stages ◽

Spider Mites ◽

Rrna Gene ◽

Associated Bacteria ◽

Operational Taxonomic Units ◽

Dominant Bacteria ◽

The 16S Rrna Gene

ABSTRACT Most arthropod-associated bacterial communities play a crucial role in host functional traits, whose structure could be dominated by endosymbionts. The spider mite Tetranychus truncatus is a notorious agricultural pest harboring various endosymbionts, yet the effects of endosymbionts on spider mite microbiota remain largely unknown. Here, using deep sequencing of the 16S rRNA gene, we characterized the microbiota of male and female T. truncatus with different endosymbionts (Wolbachia and Spiroplasma) across different developmental stages. Although the spider mite microbiota composition varied across the different developmental stages, Proteobacteria were the most dominant bacteria harbored in all samples. Positive relationships among related operational taxonomic units dominated the significant coassociation networks among bacteria. Moreover, the spider mites coinfected with Wolbachia and Spiroplasma had a significantly higher daily fecundity and juvenile survival rate than the singly infected or uninfected spider mites. The possible function of spider-mite associated bacteria was discussed. Our results highlight the dynamics of spider mite microbiotas across different life stages, and the potential role of endosymbionts in shaping the microbiota of spider mites and improving host fitness.

Download Full-text

Detection and Characterization of a Multi-drug and Multi-metal Resistant Enterobacterium Pantoea sp. from Tannery Wastewater after Secondary Treatment Process

International Journal of Plant and Environment ◽

10.18811/ijpen.v2i1-2.6616 ◽

2016 ◽

Vol 2 (1 and 2) ◽

pp. 37-42 ◽

Cited By ~ 3

Author(s):

Ashutosh Yadav ◽

Abhay Raj ◽

Ram Naresh Bharagava

Keyword(s):

Toxic Metal ◽

Tannery Wastewater ◽

Contaminated Sites ◽

Diffusion Method ◽

Rrna Gene ◽

Disk Diffusion Method ◽

Gene Sequence Analysis ◽

Nutrient Agar Medium ◽

The 16S Rrna Gene

In this study, an enterobacterium was isolated from treated tannery wastewater and characterized as gram-negative, rod shaped, motile, and lactose fermenting bacterium. Further, based on the 16S rRNA gene sequence analysis, the bacterium was identified as Pantoea sp. with accession number The antibiotic and heavy KJ576899. metal resistant property of isolated bacterium was investigated by the disk diffusion method on Muller-Hinton and nutrient agar medium amended with the increasing concentrations of various toxic metal ions, respectively. Results showed that the isolated bacterium was sensitive for amikacin, ampicillin, cefepime, cetriaxone, chloramphenicol, levofloxacin, meropenem, nalidixic acid, piperacillin and tobramycin, resistant for aztreonam, carbenicillin, cefotaxime, ceftazidime, ciprofloxacin, cotrimoxazole, imepenam, moxifloxacin, streptomycin and tetracycline, but intermediate for amoxicillin and gentamicin. In addition, the bacterium also showed the Minimum Inhibitory Concentration (MIC) values of 250, 500, 160, 190, 600, 700, 500, 380, 600 and 350 μg ml-1 forCu, Cr, Cd, Co, Zn, Fe, Ni, Pb, Mo and As, respectively with a plasmid DNA of 33.5 kb. This multi-drug and multi-metal resistant bacterium can be used as a potential agent for the bioremediation of metal contaminated sites.

Download Full-text