scholarly journals Analysis of genetic variability in F2 interspecific hybrids of mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Yeni Fatmawati ◽  
Agus Budi Setiawan ◽  
Aziz Purwantoro ◽  
Dyah Weny Respatie ◽  
Chee How Teo
Keyword(s):  
Genetic Variability ◽  
Common Bean ◽  
Interspecific Hybridization ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Interspecific Hybrids ◽  
Hybridization Technique ◽  
Marker System ◽  
F2 Population ◽  
Irap Markers

Abstract. Fatmawati Y, Setiawan AB, Purwantoro A, Respatie DW, Teo CH. 2021. Analysis of genetic variability in F2 interspecific hybrids of mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system. Biodiversitas 22: 4880-4889. Mung bean (Vigna radiata L. Wilczek) categorized as one of the pivotal annual crops of Vigna genera is commonly cultivated in rotation with the cereal crops during the drought season. Conversely, to ameliorate its stunted productivity, the interspecific hybridization technique has been introduced between the mung bean and the common bean to promote genetic improvement with the breeding projects in Indonesia. However, since mung bean is a self-pollinated crop and has a narrow genetic base, the selection and improvement of a specific trait using marker-assisted selection is more challenging. Hence, a precautionary investigation is imperative to evaluate the progenies resulting from interspecific hybridization using an ideal marker. This study aimed to investigate the genetic variability of the F2 population of the interspecific mung bean hybrids using retrotransposon-based markers, particularly Inter-Retrotransposon Amplified Polymorphism (IRAP) markers. In this study, we identified retrotransposon from the mung bean genome and determined the Long Terminal Repeat (LTR) sequence using the LTR Finder. The IRAP primers were designed from a conserved region of the LTR sequence. One hundred of the F2 interspecific hybrids generated from the crossing between mung bean and common bean were successfully discriminated by IRAP markers. The IRAP marker showed high heterozygosity and moderate Polymorphic Information Content (PIC) values. The IRAP markers were able to detect genetic variability in the F2 progenies resulting from the interspecific hybridization. Cluster analysis showed that 100 of the F2 progenies were grouped into three clusters. This study demonstrated that retrotransposon-based markers can offer an effective approach for evaluating the segregation in the F2 population of intercross hybrids in the mung bean.

scholarly journals Yield and Quality of Mung Bean (Vigna radiata (l.) R. Wilczek) Seeds Produced in Poland

2017 ◽  
Vol 74 (2) ◽  
pp. 149 ◽  
Author(s):  
Kamil MISIAK ◽  
Barbara GÓRNA ◽  
Ewelina KRÓL ◽  
Roman HOŁUBOWICZ
Keyword(s):  
Common Bean ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Phaseolus Vulgaris L ◽  
Total Proteins ◽  
High Quality ◽  
Yield And Quality ◽  
The Mean ◽  
Mature Seeds

The aim of the experiment was to do field and laboratory assessments of yield and quality of mung bean (Vigna radiata (L.) R. Wilczek) seeds cultivated in Western Poland. Mean yield of seeds per plant was higher for common bean (Phaseolus vulgaris L.) than for mung one: 13.1 g and 2.58 g, respectively. The mean 1000 mung seeds weight was 50.9 g and their germination – 78 %. Germination capacities of seeds of both beans in the field were similar. Mung beans, compared to common bean, had much smaller seeds, started to bloom later and produced mature seeds later than the latter. Mung bean seeds had more total proteins and Magnesium and Copper than common bean seeds. In Western Poland, production of high quality mung bean seeds was possible.

scholarly journals Identification of Sakura interspecific hybrids in Prunus collections

2021 ◽  
Vol 285 ◽  
pp. 02006
Author(s):  
Ilya Stepanov ◽  
Ilnur Balapanov ◽  
Anna Drygina
Keyword(s):  
Genetic Structure ◽  
Genetic Analysis ◽  
Interspecific Hybridization ◽  
Interspecific Hybrids ◽  
Sweet Cherry ◽  
Issr Markers ◽  
Separate Group ◽  
Irap Markers ◽  
Hybrid Forms ◽  
Cherry Trees

The use of IRAP and ISSR markers for the genetic analysis of Cerasus and Padus samples from the NCFRCHVW collection made it possible to establish the collection genetic structure and identify interspecific hybrids of cherry trees. Clustering of genotyped samples revealed 4 main clusters: 1) Bird cherry; 2) Cherries; 3) Interspecific hybrids of sakura; 4) Sakura. Most of the hybrid forms of sakura and cherries have formed a separate group, which is different from both sour and sweet cherry varieties, and from the classic sakura varieties. Also, some samples were identified that were assigned to groups that were not typical for them. These samples include the genotype of the Sibirskaya krasavitsa bird cherry, AI72 rootstock, Podbelskaya cherry, Polskaya sakura and ornamental cherry Rexii. In general, ISSR and IRAP markers have demonstrated their effectiveness as tools for genetic analysis of Prunus collections and identification of genotypes arising in the course of interspecific hybridization.

Genetic variability and nitrogen response indices in common bean ( Phaseolus vulgaris ) cultivars under contrasting nitrogen environments

2021 ◽  
Author(s):  
Douglas M. Zeffa ◽  
Vânia Moda‐Cirino ◽  
Alison F. Nogueira ◽  
Jéssica Delfini ◽  
Isabella M. Arruda ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Genetic Variability ◽  
Common Bean ◽  
Nitrogen Response

Linking changes in chlorophyll a fluorescence with drought stress susceptibility in mung bean [ Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
Hussan Bano ◽  
Habib‐ur‐Rehman Athar ◽  
Zafar Ullah Zafar ◽  
Hazem M. Kalaji ◽  
Muhammad Ashraf
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Stress Susceptibility

Nanoplastic ingestion induces behavioral disorders in terrestrial snails: trophic transfer effects via vascular plants

2020 ◽  
Vol 7 (3) ◽  
pp. 975-983 ◽  
Author(s):  
Yooeun Chae ◽  
Youn-Joo An
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Vascular Plants ◽  
Trophic Transfer ◽  
Achatina Fulica ◽  
Transfer Effects ◽  
Terrestrial Environment ◽  
Plastic Debris ◽  
Terrestrial Snails ◽  
African Giant Snail

This study investigated the transfer of plastic debris in a terrestrial environment from the soil to a plant (the mung bean, Vigna radiata), and then to a consumer (the African giant snail, Achatina fulica).

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