The analysis of some herds of imported cattle under the conditions of Russian farms had been shown that the genetic potential of imported cattle in many farms has being realized only by 57 %, with the output of up to 50 % of the cattle during the first 2 years. The reason for this is the discrepancy between the conditions of the biological needs of animals. In this regard, the study of factors that affect the provision of comfortable conditions for animals plays an important role. This is especially true for Holstein animals, which are more susceptible to various diseases. In accordance with the technological solutions used in modern large complexes, the loose housing method of maintenance is mainly used. This method allows the animal to move freely, providing access to the consumption of water and feed, as well as timely rest. This determines the importance of the requirements for the size of the boxes for the rest of animals, which should ensure dryness and cleanliness in the process of resting cows, prevent damage to animals, as well as the ability of other animals to displace each other. The crossbars should not interfere with the free movement of the cow in the process of lowering to the floor and getting up due to their flexibility. The dimensions of the boxes should be made taking into account the measurements of the animals’ torso, as well as the amplitude of movement in the process of lowering and rising. The purpose of the research was to study the size of boxes and their compliance with the biological needs of highly productive imported cattle. An analysis of the compliance of conditions for housing highly productive cattle of Canadian and Australian origin with their biological needs under the conditions of a mega farm has been presented in the paper. Calculation on the basis of measurements of animals has been shown that animals of the Canadian selection require boxes with a total length of at least 279 cm, Australian – 271 cm. The difference with the required width of the box has been also established. For the group of cows of Canadian selection it should be at least 120 cm, for the Australian – 114 cm. Research results have been shown that the discrepancy in the design of boxes for comfortable rest of animals is a deterrent to the realization of their genetic productive potential.