Biofunctionalization of gold nanorods

2010 ◽  
Vol 83 (1) ◽  
pp. 233-241 ◽  
Author(s):  
Sung-Yeon Hwang ◽  
Andrea R. Tao
Keyword(s):  
Gene Therapy ◽  
Cancer Detection ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Chemical Nature ◽  
Biological Fluids ◽  
Optical Measurements ◽  
Chemical Modifications ◽  
Surface Chemistries

Gold nanorods (NRs) are promising nanomaterials for biotechnology innovations that include photoassisted drug delivery, gene therapy, noninvasive cancer detection, and ultrasensitive biodetection. Owing to their unique geometry, Au NRs exhibit surface plasmon (SP) modes in the near-infrared (IR) wavelength range—ideal for carrying out optical measurements in biological fluids and tissue. Because NR interactions highly depend on the chemical nature of their solvent-accessible interface, it is necessary to carry out specific postsynthetic chemical modifications of the Au surface to create NRs that are biocompatible and biofunctional. In this review, we discuss various NR surface chemistries that have successfully enabled the integration of Au NRs into biological environments, as well as current challenges in designing the biofunctional NR interface for in vivo applications.

Folic Acid Functionalized Chlorin e6-Superparamagnetic Iron Oxide Nanocarriers as a Theranostic Agent for MRI-Guided Photodynamic Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Iron Oxide ◽  
Near Infrared ◽  
Biological Fluids ◽  
Superparamagnetic Iron Oxide ◽  
Chlorin E6 ◽  
Theranostic Agent

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.

In Vivo Monitoring of Intravenously Injected Gold Nanorods Using Near‐Infrared Light

Small ◽  
2008 ◽  
Vol 4 (7) ◽  
pp. 1001-1007 ◽  
Author(s):  
Takuro Niidome ◽  
Yasuyuki Akiyama ◽  
Kohei Shimoda ◽  
Takahito Kawano ◽  
Takeshi Mori ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Near Infrared ◽  
Infrared Light ◽  
Near Infrared Light ◽  
In Vivo Monitoring

scholarly journals Potential of Polymeric Films Loaded with Gold Nanorods for Local Hyperthermia Applications

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 582 ◽  
Author(s):  
Álvaro Cárcamo-Martínez ◽  
Juan Domínguez-Robles ◽  
Brónach Mallon ◽  
Md. Taifur Raman ◽  
Ana Sara Cordeiro ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Near Infrared ◽  
Infrared Light ◽  
Skin Model ◽  
Porcine Skin ◽  
Swelling Properties ◽  
Adhesion Properties ◽  
Local Hyperthermia ◽  
Burning Pain

Current strategies for the treatment of superficial non-melanoma skin cancer (NMSC) lesions include topical imoquimod, 5-fluorouracil, and photodynamic therapy. Although these treatments are effective, burning pain, blistering, and dermatitis have been reported as frequent side effects, making these therapies far from ideal. Plasmonic materials have been investigated for the induction of hyperthermia and use in cancer treatment. In this sense, the effectiveness of intratumorally and systemically injected gold nanorods (GnRs) in inducing cancer cell death upon near-infrared light irradiation has been confirmed. However, the in vivo long-term toxicity of these particles has not yet been fully documented. In the present manuscript, GnRs were included in a crosslinked polymeric film, evaluating their mechanical, swelling, and adhesion properties; moreover, their ability to heat up neonatal porcine skin (such as a skin model) upon irradiation was tested. Inclusion of GnRs into the films did not affect mechanical or swelling properties. GnRs were not released after film swelling, as they remained entrapped in the polymeric network; moreover, films did not adhere to porcine skin, altogether showing the enhanced biocompatibility of the material. GnR-loaded films were able to heat up the skin model over 40 °C, confirming the potential of this system for non-invasive local hyperthermia applications.

Near Infrared Raman Spectroscopy for Cancer Detection in vivo

2002 ◽  
Author(s):  
Anita Mahadevan-Jansen ◽  
Amy Robichaux ◽  
Chad Lieber ◽  
Heidi Shappell ◽  
Darryl Ellis ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Detection ◽  
Near Infrared

scholarly journals Efficacy, long-term toxicity, and mechanistic studies of gold nanorods photothermal therapy of cancer in xenograft mice

2017 ◽  
Vol 114 (15) ◽  
pp. E3110-E3118 ◽  
Author(s):  
Moustafa R. K. Ali ◽  
Mohammad Aminur Rahman ◽  
Yue Wu ◽  
Tiegang Han ◽  
Xianghong Peng ◽  
...  
Keyword(s):  
Cell Death ◽  
Mechanism Of Action ◽  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Infrared Light ◽  
Mouse Tumor ◽  
Maximal Induction

Gold nanorods (AuNRs)-assisted plasmonic photothermal therapy (AuNRs-PPTT) is a promising strategy for combating cancer in which AuNRs absorb near-infrared light and convert it into heat, causing cell death mainly by apoptosis and/or necrosis. Developing a valid PPTT that induces cancer cell apoptosis and avoids necrosis in vivo and exploring its molecular mechanism of action is of great importance. Furthermore, assessment of the long-term fate of the AuNRs after treatment is critical for clinical use. We first optimized the size, surface modification [rifampicin (RF) conjugation], and concentration (2.5 nM) of AuNRs and the PPTT laser power (2 W/cm2) to achieve maximal induction of apoptosis. Second, we studied the potential mechanism of action of AuNRs-PPTT using quantitative proteomic analysis in mouse tumor tissues. Several death pathways were identified, mainly involving apoptosis and cell death by releasing neutrophil extracellular traps (NETs) (NETosis), which were more obvious upon PPTT using RF-conjugated AuNRs (AuNRs@RF) than with polyethylene glycol thiol-conjugated AuNRs. Cytochrome c and p53-related apoptosis mechanisms were identified as contributing to the enhanced effect of PPTT with AuNRs@RF. Furthermore, Pin1 and IL18-related signaling contributed to the observed perturbation of the NETosis pathway by PPTT with AuNRs@RF. Third, we report a 15-month toxicity study that showed no long-term toxicity of AuNRs in vivo. Together, these data demonstrate that our AuNRs-PPTT platform is effective and safe for cancer therapy in mouse models. These findings provide a strong framework for the translation of PPTT to the clinic.

Polysarcosine brush stabilized gold nanorods for in vivo near-infrared photothermal tumor therapy

2017 ◽  
Vol 50 ◽  
pp. 534-545 ◽  
Author(s):  
Hong Zhu ◽  
Ying Chen ◽  
Fang-Jie Yan ◽  
Jin Chen ◽  
Xin-Feng Tao ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Near Infrared ◽  
Tumor Therapy

scholarly journals DARPin_9-29-Targeted Gold Nanorods Selectively Suppress HER2-Positive Tumor Growth in Mice

Cancers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 5235
Author(s):  
Galina M. Proshkina ◽  
Elena I. Shramova ◽  
Marya V. Shilova ◽  
Ivan V. Zelepukin ◽  
Victoria O. Shipunova ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Targeted Delivery ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Therapeutic Potential ◽  
Infrared Light ◽  
Strong Reduction ◽  
Her2 Positive ◽  
Excellent Stability

Near-infrared phototherapy has great therapeutic potential for cancer treatment. However, for efficient application, in vivo photothermal agents should demonstrate excellent stability in blood and targeted delivery to pathological tissue. Here, we demonstrated that stable bovine serum albumin-coated gold mini nanorods conjugated to a HER2-specific designed ankyrin repeat protein, DARPin_9-29, selectively accumulate in HER2-positive xenograft tumors in mice and lead to a strong reduction in the tumor size when being illuminated with near-infrared light. The results pave the way for the development of novel DARPin-based targeted photothermal therapy of cancer.

scholarly journals Photoacoustic molecular imaging using combined acupuncture and gold nanorods as a composite contrast agent

2019 ◽  
Vol 12 (03) ◽  
pp. 1941004
Author(s):  
Dan Wu ◽  
Xinxin Zhang ◽  
Jian Rong ◽  
Huabei Jiang
Keyword(s):  
Contrast Agent ◽  
Contrast Enhancement ◽  
Mouse Brain ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Local Concentration ◽  
Quantitative Results ◽  
Zusanli Acupoint

In this study, we developed a novel photoacoustic imaging technique based on poly (ethyleneglycol)-coated (PEGylated) gold nanorods (PEG-GNRs) (as the contrast agent) combined with traditional Chinese medicine (TCM) acupuncture (as the auxiliary method) for quantitatively monitoring contrast enhancement in the vasculature of a mouse brain in vivo. This study takes advantage of the strong near-infrared absorption (peak at [Formula: see text][Formula: see text]nm) of GNRs and the ability to adjust the hemodynamics of acupuncture. Experimental results show that photoacoustic tomography (PAT) successfully reveals the optical absorption variation of the vasculature of the mouse brain in response to intravenous administration of GNRs and acupuncture at the Zusanli acupoint (ST36) both individually and combined. The quantitative measurement of contrast enhancement indicates that the composite contrast agents (integration of acupuncture and GNRs) would greatly enhance the photoacoustic imaging contrast. The quantitative results also have the potential to estimate the local concentration of GNRs and even the real-time effects of acupuncture.

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