Zoliflodacin. DNA gyrase subunit B (GyrB) (bacterial) inhibitor, Treatment of uncomplicated gonorrhea

2019 ◽  
Vol 44 (12) ◽  
pp. 943
Author(s):  
R. Thakare ◽  
A. Dasgupta ◽  
S. Chopra
Keyword(s):  
Dna Gyrase ◽  
Subunit B ◽  
Inhibitor Treatment ◽  
Uncomplicated Gonorrhea

scholarly journals Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanan Duan ◽  
Ran Chen ◽  
Rong Zhang ◽  
Weitao Jiang ◽  
Xuesen Chen ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Fermentation Broth ◽  
Biological Control Agent ◽  
Lateral Roots ◽  
Dna Gyrase ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Fertilizer Treatment ◽  
Strong Inhibitory Effect ◽  
Subunit B

Apple replant disease (ARD) is a common problem in major apple planting areas, and biological factors play a leading role in its etiology. Here, we isolated the bacterial strain QSB-6 from the rhizosphere soil of healthy apple trees in a replanted orchard using the serial dilution method. Strain QSB-6 was provisionally identified as Bacillus amyloliquefaciens based on its morphology, physiological and biochemical characteristics, carbon source utilization, and chemical sensitivity. Maximum likelihood analysis based on four gene sequences [16S ribosomal RNA gene (16S rDNA), DNA gyrase subunit A (gyrA), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB)] from QSB-6 and other strains indicated that it had 100% homology with B. amyloliquefaciens, thereby confirming its identification. Flat standoff tests showed that strain QSB-6 had a strong inhibitory effect on Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Fusarium oxysporum, Alternaria alternata, Aspergillus flavus, Phoma sp., Valsa mali, Rhizoctonia solani, Penicillium brasilianum, and Albifimbria verrucaria, and it had broad-spectrum antibacterial characteristics. Extracellular metabolites from strain QSB-6 showed a strong inhibitory effect on Fusarium hyphal growth and spore germination, causing irregular swelling, atrophy, rupture, and cytoplasmic leakage of fungal hyphae. Analysis of its metabolites showed that 1,2-benzenedicarboxylic acid and benzeneacetic acid, 3- hydroxy-, methyl ester had good inhibitory effects on Fusarium, and increased the length of primary roots and the number of lateral roots of Arabidopsis thaliana plantlet. Pot experiments demonstrated that a QSB-6 bacterial fertilizer treatment (T2) significantly improved the growth of Malus hupehensis Rehd. seedlings. It increased root length, surface area, tips, and forks, respiration rate, protective enzyme activities, and the number of soil bacteria while reducing the number of soil fungi. Fermentation broth from strain QSB-6 effectively prevented root damage from Fusarium. terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (qPCR) assays showed that the T2 treatment significantly reduced the abundance of Fusarium in the soil and altered the soil fungal community structure. In summary, B. amyloliquefaciens QSB-6 has a good inhibitory effect on Fusarium in the soil and can significantly promote plant root growth. It has great potential as a biological control agent against ARD.

Novel Benzotriazole acetamide derivatives as Benzo-Fused Five-Membered Nitrogen-Containing Heterocycle- Insilico Screening, Molecular Docking, and Synthesis

2021 ◽  
Vol 18 ◽  
Author(s):  
Patil Tejaswini D. ◽  
Amrutkar Sunil V.
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Docking ◽  
Active Site ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Dna Gyrase ◽  
Antibacterial Drug ◽  
E Coli ◽  
Fast Development ◽  
Subunit B

Background: DNA gyrase subunit B (1KZN) is an attractive target for antibacterial drug development because of its role in DNA replication. The fast development of antimicrobial medication resistance necessitates the quick discovery of new antimicrobial medicines. Objective: The goal of this research is to design, synthesize, and discover benzo-fused five-membered nitrogen-containing heterocycles that bind to DNA gyrase subunit B via molecular docking (1KZN). Methods: Based on literature research, 2-(1H-1,2,3-Benzotriazol-1-yl)-N-substituted acetamide was synthesized using an efficient method. All synthesized compounds were evaluated for antibacterial activity against three distinct organisms: E. coli, Pseudomonas aeruginosa, Staphylococcus aureus. In a docking investigation, the chemical interacts with the active site of DNA gyrase subunit B (1KZN), indicating that it might have antibacterial action. Conclusion: According to the findings of this research, the compounds 3d and 3f show antibacterial properties. For Staphylococcus aureus, 3c has the potential to be an antibacterial agent.

Transformation of Neisseria gonorrhoeae: physical requirements of the transforming DNA

1991 ◽  
Vol 37 (5) ◽  
pp. 345-349 ◽  
Author(s):  
Daniel C. Stein
Keyword(s):  
Nalidixic Acid ◽  
Dna Sequences ◽  
Acid Resistance ◽  
Dna Gyrase ◽  
Base Pair Fragment ◽  
Modification Phenotype ◽  
Specific Uptake ◽  
Subunit B ◽  
Pair Fragment ◽  
Nalidixic Acid Resistance

The 1600-bp (base pair) fragment encoding a portion of the nalidixic acid resistant DNA gyrase, subunit B, was characterized to determine what parameters effect transformation in the gonococcus. When this DNA (pSY2) was isolated from Escherichia coli, it was able to transform a variety of gonococcal strains to resistance to nalidixic acid via DNA-mediated transformation, irrespective of their restriction–modification phenotype. Nalidixic acid resistant transformants contained no plasmid DNA sequences that corresponded to the vector, as measured by plasmid screening procedures and colony hybridization techniques. Supercoiled and linear DNA transformed the gonococcus at the same efficiency. DNA fragments as small as 615 bp were able to transform the gonococcus. The presence of a 10-bp uptake sequence enhanced a DNA fragment's ability to transform the gonococcus by four orders of magnitude. When the fragment encoding the nalidixic acid resistant DNA gyrase was subcloned into M13mp18, both the replicative form and the single-stranded form of the phage were able to transform the gonococcus to nalidixic acid resistance. Key words: sequence-specific uptake, gyrase, restriction and modification.

scholarly journals Antagonism between novobiocin and coumermycin A1inBacillus subtilis

1984 ◽  
Vol 44 (3) ◽  
pp. 243-250 ◽  
Author(s):  
István Gadó ◽  
Valéria Széll ◽  
Kálmán Büki ◽  
György Szvoboda
Keyword(s):  
Opposite Direction ◽  
Dna Gyrase ◽  
Alternative Models ◽  
Inhibiting Effect ◽  
Subinhibitory Concentrations ◽  
Subunit B ◽  
Growth Inhibiting

SUMMARYWhen combinations of inhibitors acting on the subunit B of DNA gyrase were tested inBac.subtilisstrains, the growth-inhibiting effect of novobiocin was specifically antagonized by subinhibitory concentrations of coumermycin A1. An antagonism in the opposite direction was not observed.Two alternative models are proposed, where the supercoiling decrease caused by novobiocin is antagonized by coumermycin.This phenomenon seems to be characteristic of theBac.subtilisspecies.

Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene

2006 ◽  
Vol 55 (4) ◽  
pp. 263-268 ◽  
Author(s):  
Julien Delmas ◽  
Franck Breysse ◽  
Gregory Devulder ◽  
Jean-Pierre Flandrois ◽  
Monique Chomarat
Keyword(s):  
Dna Gyrase ◽  
Rapid Identification ◽  
Encoding Gene ◽  
Subunit B

scholarly journals Virtual Screening to Identify the Protein Network Interaction of Hypericin with Red Complex Pathogens

2021 ◽  
pp. 341-352
Author(s):  
C. Pratheebha ◽  
Jayaseelan Vijayshree Priyadharsini ◽  
A. S. Smiline Girija ◽  
P. Sankar Ganesh ◽  
Nidhi Poddar
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Porphyromonas Gingivalis ◽  
Heat Shock Protein 90 ◽  
Dna Gyrase ◽  
Protein Network ◽  
Treponema Denticola ◽  
Network Interaction ◽  
Wide Range ◽  
Subunit B

Introduction: Hypericin is the anthraquinone derivative and has many properties like antiviral, antifungal and antibacterial. The red complex pathogens which include Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in association with other microbes found in the periodontal pockets, cause severe inflammation resulting in periodontitis. Novel bioactive agents from several sources have been tested against the microbial pathogens to deduce antimicrobial activity.  Aim: The aim of the study is to virtually screen and identify the protein network interaction of hypericin in red complex pathogens. Methodology: The STITCH v5.0 pipeline was primarily used to identify the drug-protein interactions. The VirulentPred and VICMPred software were used for elucidating the functional class of the proteins and virulence property. The sub cellular localization of virulent proteins was analysed with pSORTb v3.0 software. Further, the epitopes in virulent proteins were identified using BepiPred v1.0 linear epitope prediction tool. Results: Heat shock protein 90 of Porphyromonas gingivalis were found to involve in the cellular process and DNA topoisomerase IV subunit B, heat shock protein 90, DNA gyrase subunit A and DNA gyrase subunit B of Treponema denticola were found to be the virulent factors. The virulent proteins were located in the cytoplasm, which would further increase the potential effect of the drug to serve as antimicrobial agents. Finally, epitopes were predicted on the virulent proteins which can be specifically docked to further ascertain their interactions with the phytocompound. Conclusion: Hypericin with all its potential and biological benefits can be addressed, can be used as an antimicrobial agent to eradicate dental pathogens which are recalcitrant to treatment. The mode of action of hypericin is, it is targeting crucial proteins in red complex pathogens. Further in vitro studies should be performed on a wide range of pathogens to substantiate the true interactions between the drugs and the protein repertoire of pathogens.

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