Fidgetin-Like 1 Gene Inhibited by Basic Fibroblast Growth Factor Regulates the Proliferation and Differentiation of Osteoblasts

Basic fibroblast growth factor (bFGF) stimulated the sustained proliferation of mouse epidermal melanoblasts derived from epidermal cell suspensions in a serum-free medium supplemented with dibutyryl adenosine 3′,5′-cyclic monophosphate (DBcAMP). The melanoblasts could be subcultured in the serum-free medium supplemented with the two factors in the presence of keratinocytes, but not in the absence of keratinocytes. In these conditions, some melanoblasts proliferated without differentiating for more than 20 days including a subculture. This is the first report of a successful culture of melanoblasts from mammalian skin. This culture system is expected to clarify further markers for melanoblasts and requirements for their proliferation and differentiation.

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Influence of basic fibroblast growth factor in the solution and adsorbed form on the proliferation and differentiation of cells

Inflammation and Regeneration ◽

10.2492/inflammregen.26.181 ◽

2006 ◽

Vol 26 (3) ◽

pp. 181-184 ◽

Cited By ~ 2

Author(s):

Sachiko Inoue ◽

Yasuhiko Tabata

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Fibroblast Growth ◽

Proliferation And Differentiation

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Generation of a Primitive Erythroid Cell Line and Promotion of Its Growth by Basic Fibroblast Growth Factor

Blood ◽

10.1182/blood.v91.9.3202.3202_3202_3209 ◽

1998 ◽

Vol 91 (9) ◽

pp. 3202-3209 ◽

Cited By ~ 1

Author(s):

David Yuen ◽

Leena Mittal ◽

Chu-Xia Deng ◽

Kyunghee Choi

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Cell Line ◽

Basic Fibroblast Growth Factor ◽

Es Cells ◽

Embryoid Bodies ◽

Erythroid Cell ◽

Fibroblast Growth ◽

Proliferation And Differentiation

An immortalized cell line representing the primitive erythroid (EryP) lineage was established from in vitro–differentiated progeny (embryoid bodies [EBs]) of embryonic stem (ES) cells using a retroviral insertional mutation, and has been termed EB-PE for embryoid body–derived primitive erythroid. Even though EB-PE cells are immortalized, they show characteristics of normal EryP cells, such as gene expression and growth factor dependency. In addition, EB-PE cells can differentiate further in culture. Investigation of growth factor requirements of EB-PE cells showed that basic fibroblast growth factor (bFGF) and erythropoietin (Epo) play unique roles in EB-PE proliferation and differentiation. While bFGF was a strong mitogen, Epo was required for both proliferation and differentiation. The unique proliferative response to bFGF coincided with upregulation of its receptor, fibroblast growth factor receptor (fgfr-1), and downregulation of erythropoietin receptor (EpoR) gene expression. Studies of primary EryP cells derived from early EBs, when tested in a colony-formation assay, also provided evidence for the mitogenic role of bFGF in concert with Epo.

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Generation of a Primitive Erythroid Cell Line and Promotion of Its Growth by Basic Fibroblast Growth Factor

Blood ◽

10.1182/blood.v91.9.3202 ◽

1998 ◽

Vol 91 (9) ◽

pp. 3202-3209 ◽

Cited By ~ 12

Author(s):

David Yuen ◽

Leena Mittal ◽

Chu-Xia Deng ◽

Kyunghee Choi

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Cell Line ◽

Basic Fibroblast Growth Factor ◽

Es Cells ◽

Embryoid Bodies ◽

Erythroid Cell ◽

Fibroblast Growth ◽

Proliferation And Differentiation

Abstract An immortalized cell line representing the primitive erythroid (EryP) lineage was established from in vitro–differentiated progeny (embryoid bodies [EBs]) of embryonic stem (ES) cells using a retroviral insertional mutation, and has been termed EB-PE for embryoid body–derived primitive erythroid. Even though EB-PE cells are immortalized, they show characteristics of normal EryP cells, such as gene expression and growth factor dependency. In addition, EB-PE cells can differentiate further in culture. Investigation of growth factor requirements of EB-PE cells showed that basic fibroblast growth factor (bFGF) and erythropoietin (Epo) play unique roles in EB-PE proliferation and differentiation. While bFGF was a strong mitogen, Epo was required for both proliferation and differentiation. The unique proliferative response to bFGF coincided with upregulation of its receptor, fibroblast growth factor receptor (fgfr-1), and downregulation of erythropoietin receptor (EpoR) gene expression. Studies of primary EryP cells derived from early EBs, when tested in a colony-formation assay, also provided evidence for the mitogenic role of bFGF in concert with Epo.

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