Detection of Sialic Acid Residues and Studies of Their Organization in Normal and Tumor α1-Acid Glycoproteins as Probed by Surface-Enhanced Raman Spectroscopy

1993 ◽  
Vol 47 (5) ◽  
pp. 535-538 ◽  
Author(s):  
Konstantin V. Sokolov ◽  
Nargiz E. Byramova ◽  
Larisa V. Mochalova ◽  
Alexander B. Tuzikov ◽  
Svetlana D. Shiyan ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Surface Enhanced Raman Spectroscopy ◽  
Tumor Patients ◽  
Sugar Chain ◽  
Healthy Donors ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Chain Organization ◽  
Sugar Chains ◽  
Sialylated Glycoproteins

Surface-enhanced Raman scattering (SERS) spectra of sialic acid (SA) benzyl, and methyl glycosides as well as natural sialylated glycoproteins from human cells of healthy donors and tumor patients have been analyzed in view of the fact that peripheral fragments of many bioactive glycoconjugates are SA residues. SA residues can be detected by SERS at concentrations as low as 10−6 M. The pattern of interaction of SA with the surface of a silver hydrosol involves the C-8 and C-9 hydroxy groups of SA. SERS spectroscopy is sensitive to changes in the content and type of branching of sialylated sugar chains in sialylglycoproteins. The differences in sialylated sugar-chain organization for α1-acid glycoproteins from healthy donors and tumor patients have been detected by means of SERS spectroscopy. The first example of the detection of SA residues for a suspension of living cells has been presented.

scholarly journals Determination of sialic acid in saliva by means of surface-enhanced Raman spectroscopy as a marker in adnexal mass patients: ovarian cancer vs benign cases

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
José de Jesús Zermeño-Nava ◽  
Marco Ulises Martínez-Martínez ◽  
Ana Laura Rámirez-de-Ávila ◽  
Aida Catalina Hernández-Arteaga ◽  
Ma. Guadalupe García-Valdivieso ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Raman Spectroscopy ◽  
Sialic Acid ◽  
Adnexal Mass ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Diagnosis of breast cancer by analysis of sialic acid concentrations in human saliva by surface-enhanced Raman spectroscopy of silver nanoparticles

Nano Research ◽  
2017 ◽  
Vol 10 (11) ◽  
pp. 3662-3670 ◽  
Author(s):  
Aida Hernández-Arteaga ◽  
José de Jesús Zermeño Nava ◽  
Eleazar Samuel Kolosovas-Machuca ◽  
J. Jesús Velázquez-Salazar ◽  
Ekaterina Vinogradova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Sialic Acid ◽  
Surface Enhanced Raman Spectroscopy ◽  
Human Saliva ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis

Oral Diseases ◽  
2019 ◽  
Vol 25 (6) ◽  
pp. 1627-1633 ◽  
Author(s):  
Alondra Hernández‐Cedillo ◽  
Ma. Guadalupe García-Valdivieso ◽  
Aida Catalina Hernández‐Arteaga ◽  
Nuria Patiño-Marín ◽  
Ángel Antonio Vértiz-Hernández ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Sialic Acid ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Development of a Tri-Functional Nanoprobe for Background-Free SERS Detection of Sialic Acid on the Cell Surface

Chemosensors ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 92
Author(s):  
Septila Renata ◽  
Nitish Verma ◽  
Zhijay Tu ◽  
Rong-Long Pan ◽  
Mario Hofmann ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Phenylboronic Acid ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Silver Nanocubes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Cellular Diagnostics

Sialic acid (SA) on the surface of cells is indispensable in numerous physiological and pathological processes, and sensitive and reproducible detection of SA is crucial for diagnosis and therapy in many diseases. Here, we developed a tri-functional nanoprobe as a sensitive and straightforward surface-enhanced Raman spectroscopy (SERS) nanoprobe for sialoglycan detection on cell surfaces. The reporter was designed to provide three key functionalities that make it ideal for SA detection. First, we employed two recognition groups, phenylboronic acid and an ammonium group, that enhance SA recognition and capture efficiency. Second, we used cyano as the Raman reporter because it emits in the cellular Raman silent region. Finally, thiol acted as an anchoring agent to conjugate the reporter to silver nanocubes to provide SERS enhancement. Our molecular nanoprobe design demonstrated the ability to detect SA on the cell surface with high sensitivity and spatial resolution, opening up new routes to cellular diagnostics.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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