scholarly journals Phase-related differences in egg production of the migratory locust regulated by differential oosorption through microRNA-34 targeting activinβ

PLoS Genetics ◽  
2021 ◽  
Vol 17 (1) ◽  
pp. e1009174
Author(s):  
Lianfeng Zhao ◽  
Wei Guo ◽  
Feng Jiang ◽  
Jing He ◽  
Hongran Liu ◽  
...  
Keyword(s):  
Egg Production ◽  
Regulatory Mechanism ◽  
Locusta Migratoria ◽  
Expression Patterns ◽  
Migratory Locust ◽  
Egg Number ◽  
Gregarious Locusts ◽  
Terminal Oocyte ◽  
Mirna Targeting

Outbreaks of locust plagues result from the long-term accumulation of high-density egg production. The migratory locust, Locusta migratoria, displays dramatic differences in the egg-laid number with dependence on population density, while solitarious locusts lay more eggs compared to gregarious ones. However, the regulatory mechanism for the egg-laid number difference is unclear. Herein, we confirm that oosorption plays a crucial role in the regulation of egg number through the comparison of physiological and molecular biological profiles in gregarious and solitarious locusts. We find that gregarious oocytes display a 15% higher oosorption ratio than solitarious ones. Activinβ (Actβ) is the most highly upregulated gene in the gregarious terminal oocyte (GTO) compared to solitarious terminal oocyte (STO). Meanwhile, Actβ increases sharply from the normal oocyte (N) to resorption body 1 (RB1) stage during oosorption. The knockdown of Actβ significantly reduces the oosorption ratio by 13% in gregarious locusts, resulting in an increase in the egg-laid number. Based on bioinformatic prediction and experimental verification, microRNA-34 with three isoforms can target Actβ. The microRNAs display higher expression levels in STO than those in GTO and contrasting expression patterns of Actβ from the N to RB1 transition. Overexpression of each miR-34 isoform leads to decreased Actβ levels and significantly reduces the oosorption ratio in gregarious locusts. In contrast, inhibition of the miR-34 isoforms results in increased Actβ levels and eventually elevates the oosorption ratio of solitarious locusts. Our study reports an undescribed mechanism of oosorption through miRNA targeting of a TGFβ ligand and provides new insights into the mechanism of density-dependent reproductive adaption in insects.

scholarly journals SURVIVAL OF MIGRATORY LOCUST AT DIFFERENT KEEPING CONDITIONS

2020 ◽  
Author(s):  
A.B. Gerus ◽  
◽  
Y.S. Tokarev ◽  
G.R. Lednev ◽  
M.B. Levchenko ◽  
...  
Keyword(s):  
Survival Rate ◽  
Locusta Migratoria ◽  
Migratory Locust ◽  
Locusta Migratoria Migratorioides ◽  
Gregarious Locusts

In this article we studied the conditions for keeping two species of gregarious locusts: the African migratory locust (Locusta migratoria migratorioides) and the Asian migratory locust (Locusta migratoria migratoria) in open and shaded areas. Based on the data obtained, it is shown that the survival rate of insects of the non-diapausal subspecies was higher in comparison with the obligate monovoltine.

scholarly journals Juvenile hormone acts through FoxO to promote Cdc2 and Orc5 transcription for polyploidy-dependent vitellogenesis

Development ◽  
2020 ◽  
Vol 147 (18) ◽  
pp. dev188813
Author(s):  
Zhongxia Wu ◽  
Qiongjie He ◽  
Baojuan Zeng ◽  
Haodan Zhou ◽  
Shutang Zhou
Keyword(s):  
Juvenile Hormone ◽  
Egg Production ◽  
Large Scale ◽  
Origin Recognition Complex ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Upstream Region ◽  
Migratory Locust ◽  
Phosphatase 2A ◽  
Underlying Mechanisms

ABSTRACTVitellogenin (Vg) is a prerequisite for egg production and embryonic development after ovipositioning in oviparous animals. In many insects, juvenile hormone (JH) promotes fat body cell polyploidization for the massive Vg synthesis required for the maturation of multiple oocytes, but the underlying mechanisms remain poorly understood. Using the migratory locust Locusta migratoria as a model system, we report here that JH induces the dephosphorylation of Forkhead box O transcription factor (FoxO) through a signaling cascade including leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase 2A (PP2A). JH promotes PP2A activity via LCMT1-mediated methylation, consequently triggering FoxO dephosphorylation. Dephosphorylated FoxO binds to the upstream region of two endocycle-related genes, cell-division-cycle 2 (Cdc2) and origin-recognition-complex subunit 5 (Orc5), and activates their transcription. Depletion of FoxO, Cdc2 or Orc5 results in blocked polyploidization of fat body cells, accompanied by markedly reduced Vg expression, impaired oocyte maturation and arrested ovarian development. The results suggest that JH acts via LCMT1-PP2A-FoxO to regulate Cdc2 and Orc5 expression, and to enhance ploidy of fat body cells in preparation for the large-scale Vg synthesis required for synchronous maturation of multiple eggs.

Genetic Variation and Population Structure of Oriental Migratory Locust, Locusta migratoria manilensis, in China by Allozyme, SSRP-PCR, and AFLP Markers

2006 ◽  
Vol 44 (7-8) ◽  
pp. 332-346 ◽  
Author(s):  
Xianyun Zheng ◽  
Yang Zhong ◽  
Yihao Duan ◽  
Chunxuan Li ◽  
Li Dang ◽  
...  
Keyword(s):  
Population Structure ◽  
Genetic Variation ◽  
Locusta Migratoria ◽  
Aflp Markers ◽  
Migratory Locust ◽  
Locusta Migratoria Manilensis

scholarly journals An Endogenous RNA Transcript Antisense to CNGα1 Cation Channel mRNA

2002 ◽  
Vol 13 (10) ◽  
pp. 3696-3705 ◽  
Author(s):  
Chin-Hung Cheng ◽  
David Tai-Wai Yew ◽  
Hiu-Yee Kwan ◽  
Qing Zhou ◽  
Yu Huang ◽  
...  
Keyword(s):  
Expression System ◽  
Expression Patterns ◽  
Long Term Potentiation ◽  
Information Storage ◽  
Term Potentiation ◽  
Hippocampal Ca1 ◽  
Oocyte Expression System ◽  
Neuronal Functions ◽  
Rna Transcript

CNG channels are cyclic nucleotide-gated Ca2+-permeable channels that are suggested to be involved in the activity-dependent alterations of synaptic strength that are thought to underlie information storage in the CNS. In this study, we isolated an endogenous RNA transcript antisense to CNGα1 mRNA. This transcript was capable of down-regulating the expression of sense CNGα1 in theXenopus oocyte expression system. RT-PCR, Northern blot, and in situ hybridization analyses showed that the transcript was coexpressed with CNGα1 mRNA in many regions of human brain, notably in those regions that were involved in long-term potentiation and long-term depression, such as hippocampal CA1 and CA3, dentate gyrus, and cerebellar Purkinje layer. Comparison of expression patterns between adult and fetal cerebral cortex revealed that there were concurrent developmental changes in the expression levels of anti-CNG1 and CNGα1. Treatment of human glioma cell T98 with thyroid hormone T3 caused a significant increase in anti-CNG1 expression and a parallel decrease in sense CNGα1 expression. These data suggest that the suppression of CNGα1 expression by anti-CNG1 may play an important role in neuronal functions, especially in synaptic plasticity and cortical development. Endogenous antisense RNA-mediated regulation may represent a new mechanism through which the activity of ion channels can be regulated in the human CNS.

scholarly journals SAPU-SAPU FISH (Hyposarcus pardalis) AS A SINGLE PROTEIN SOURCE FOR LAYING MOJOSARI DUCKS

2016 ◽  
Vol 41 (3) ◽  
pp. 117-124 ◽  
Author(s):  
B. Indarsih ◽  
A. Asnawi ◽  
D. K. Purnamasari
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Egg Production ◽  
Egg Quality ◽  
Production Performance ◽  
Protein Source ◽  
Egg Mass ◽  
Final Body Weight ◽  
Egg Number ◽  
Single Protein

The present study was conducted to evaluate the optimum level of dietary inclusion and the form of feeding sapu-sapu fish (SSF) as a single protein source for feed components of small holder Mojosari duck farming. A total of 180 twenty four wk-old laying ducks were fed with two forms (sun-dried and ground-fresh) and three levels of SSF (10, 20 and 30%) from 24 to 32 wk of age. Experimental diets were formulated containing 105.1; 141.5 and 177.9 g/kg crude protein and 3078; 3065 and 3052 kcal metabolizable energy (ME) /kg diet. Production performance and egg quality were measured. The form of SSF had a significant effect (P<0.0001) on feed intake, egg production, feed conversion, egg mass and egg number except final body weight. No significant effects (P>0.05) were found on all of the performance parameters measured due to feeding levels of SSF except feed intake and final body weight. The interaction of form and feeding level was significant (P<0.05) on egg and body weights. Low performance and egg quality were observed in the birds fed a diet containing the dried SSF. It can be suggested that local ducks required 20% SSF as a single protein source for maximum egg production (41.2%) and total egg number per wk (3.1 eggs/bird/wk) during 8 wk of rearing period. Egg quality can be improved by feeding the fresh SSF. However, due to be sufficiently better processing technique, the dried SSF would be potential to replace the common fish for laying ducks.

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