scholarly journals Increased Frequencies of Th22 Cells as well as Th17 Cells in the Peripheral Blood of Patients with Ankylosing Spondylitis and Rheumatoid Arthritis

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e31000 ◽  
Author(s):  
Lei Zhang ◽  
Yong-gang Li ◽  
Yu-hua Li ◽  
Lei Qi ◽  
Xin-guang Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Th22 Cells

scholarly journals Increased Th17 and Th22 cell levels predict acute lung injury in patients with sepsis

2019 ◽  
Author(s):  
Gang Li ◽  
Liangtian Zhang ◽  
Nannan Han ◽  
Ke Zhang ◽  
Hengjie Li
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Correlation Analysis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Pearson Correlation ◽  
Characteristic Curve ◽  
Multivariate Logistic Regression Analysis ◽  
Area Under The Curve ◽  
Th22 Cells

Abstract Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 cells and Th22 cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 cells (P < 0.05), and the levels of Th17 cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) cells were independent risk factor for ALI. The area under the curve of Th22 cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 cells (P > 0.05). Conclusions: The levels of Th22 and Th17 cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.

THU0129 Selective elimination of pathogenic TH17 cells from peripheral blood of rheumatoid arthritis patients by a purified fungal polysaccharide

2013 ◽  
Vol 71 (Suppl 3) ◽  
pp. 198.3-198
Author(s):  
A. Alunno ◽  
E. Pericolini ◽  
E. Gabrielli ◽  
O. Bistoni ◽  
S. Caterbi ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Selective Elimination

Increased number of Th22 cells and correlation with Th17 cells in peripheral blood of patients with IgA nephropathy

2013 ◽  
Vol 74 (12) ◽  
pp. 1586-1591 ◽  
Author(s):  
Zhiguo Peng ◽  
Jun Tian ◽  
Xianquan Cui ◽  
Wanhua Xian ◽  
Huaibin Sun ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Th22 Cells

scholarly journals Elevated Th22 Cells Correlated with Th17 Cells in Peripheral Blood of Patients with Acute Myeloid Leukemia

2014 ◽  
Vol 15 (2) ◽  
pp. 1927-1945 ◽  
Author(s):  
Shuang Yu ◽  
Chuanfang Liu ◽  
Lei Zhang ◽  
Baozhong Shan ◽  
Tian Tian ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Myeloid Leukemia ◽  
Th22 Cells ◽  
Acute Myeloid

Elevated Th22 Cells Correlated with Th17 Cells in Patients with Rheumatoid Arthritis

2011 ◽  
Vol 31 (4) ◽  
pp. 606-614 ◽  
Author(s):  
Lei Zhang ◽  
Jian-min Li ◽  
Xin-guang Liu ◽  
Dao-xin Ma ◽  
Nai-wen Hu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Th17 Cells ◽  
Th22 Cells

Cytokine Secretion by Pathogen Recognition Receptor-stimulated Dendritic Cells in Rheumatoid Arthritis and Ankylosing Spondylitis

2012 ◽  
Vol 39 (10) ◽  
pp. 1918-1928 ◽  
Author(s):  
CLAUDIA PREVOSTO ◽  
JANE C. GOODALL ◽  
J.S. HILL GASTON
Keyword(s):  
Rheumatoid Arthritis ◽  
Dendritic Cells ◽  
Ankylosing Spondylitis ◽  
Messenger Rna ◽  
Posttranscriptional Regulation ◽  
Th17 Cells ◽  
Cytokine Secretion ◽  
Pathogen Recognition ◽  
Interleukin 23 ◽  
Factor Α

Objective.Interleukin 23 (IL-23) plays a major role in differentiation and survival of IL-17-secreting CD4+ Th17 cells. Having noted a higher frequency of Th17 cells in ankylosing spondylitis (AS) and rheumatoid arthritis (RA) than in healthy donors (HD), we investigated whether IL-23 secretion is increased in these conditions.Methods.Monocyte-derived dendritic cells (moDC) were obtained from peripheral blood of 17 HD, 16 patients with RA, and 30 patients with AS, and stimulated with ligands for several pathogen recognition receptors. Messenger RNA (mRNA) expression and cytokine secretion were analyzed by real-time polymerase chain reaction and ELISA, respectively.Results.The combination of ligands for Toll-like receptors (TLR) 7/8 and TLR3 led to synergistic secretion of both IL-23 and IL-12p70 from all subjects; similar synergy was seen with TLR2 ligands and curdlan. However, for both combinations, moDC from patients with RA produced significantly lower amounts of IL-23 than moDC from patients with AS; in contrast, IL-12p70 secretion did not differ. Similarly, tumor necrosis factor-α, IL-6, and IL-10 were secreted at comparable levels in all subjects, whereas CXCL8 and CCL3 production was actually enhanced in moDC of patients with RA. Equivalent levels of mRNA for both IL-23p19 and IL-12p35 subunits were found in moDC from all donors, suggesting posttranscriptional regulation of IL-23 production in RA.Conclusion.Our observations show that IL-23 production is decreased in RA and maintained in AS. Because increased numbers of CD4+IL-17+ T cells are seen in both diseases, these observations imply that there are different mechanisms underlying chronic inflammation in these 2 forms of inflammatory arthritis.

scholarly journals Effect of IL-34 on T helper 17 cell proliferation and IL-17 secretion by peripheral blood mononuclear cells from rheumatoid arthritis patients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xin Li ◽  
Yimeng Lei ◽  
Ziyu Gao ◽  
Bei Zhang ◽  
Liping Xia ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Transcription Factor ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Mononuclear Cells ◽  
Treg Cells ◽  
T Helper ◽  
T Helper 17 ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

AbstractInterleukin (IL)-34 is a new pro-inflammatory cytokine with elevated expression in rheumatoid arthritis (RA) patients. Our previous study showed that the frequency of T helper 17 (Th17) cells was also elevated in RA patients. Our study aimed to determine the effects of IL-34 on the proliferation, transcription factor expression and cytokine secretion of different subgroups of CD4 + T cells [Th1, Th2, Th17 and regulatory T (Treg) cells] in RA patients. Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of 10 RA patients and stimulated with different concentrations of recombinant human (rh) IL-34 (0, 25, 50 and 100 ng/ml). Flow cytometry was used to determine the frequencies of the 4 subgroups of CD4 + T cells. Reverse transcription-PCR, western blotting and enzyme-linked immunosorbent assays were used to determine the mRNA and protein expression levels of transcription factors and cytokines. As a result, the frequency of Th17 cells was obviously increased under IL-34 stimulation. Moreover, the expression of the transcription factor retinoic acid-related orphan receptor (ROR-γt) and secretion of IL-17 by PBMCs were increased by stimulation with IL-34. However, there were no effects of IL-34 on transcription factors or cytokine secretion in Th1, Th2 and Treg cells. In conclusion, IL-34 can improve the proliferation of Th17 cells and expression of IL-17 in RA patients.

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