scholarly journals Increased Th17 and Th22 cell levels predict acute lung injury in patients with sepsis

2019 ◽  
Author(s):  
Gang Li ◽  
Liangtian Zhang ◽  
Nannan Han ◽  
Ke Zhang ◽  
Hengjie Li
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Correlation Analysis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Pearson Correlation ◽  
Characteristic Curve ◽  
Multivariate Logistic Regression Analysis ◽  
Area Under The Curve ◽  
Th22 Cells

Abstract Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 cells and Th22 cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 cells (P < 0.05), and the levels of Th17 cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) cells were independent risk factor for ALI. The area under the curve of Th22 cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 cells (P > 0.05). Conclusions: The levels of Th22 and Th17 cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.

Effect of MiR-16 on Acute Lung Injury in Neonatal Rats Through Regulating NF-κB Pathway

2020 ◽  
Vol 10 (3) ◽  
pp. 360-364
Author(s):  
Yan Xing ◽  
Yuan Liang ◽  
Changsong Shi ◽  
Yudong Miao ◽  
Jianqin Gu ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Correlation Analysis ◽  
Western Blotting ◽  
Inflammatory Responses ◽  
Morphological Changes ◽  
Pearson Correlation ◽  
Mrna Levels ◽  
Low Oxygen ◽  
Pearson Correlation Analysis

Acute lung injury represents a widespread, variable type of lung injury characterized by a low oxygen level in the blood, non cardiogenic pulmonary edema, low lung compliance and extensive capillary leakage. In our study, the Wistar rat mode of ALI was established using lipopolysaccharide (LPS). The rats were randomly divided into normal control (NC) group (n = 12) and miR-16 overex-pression group (n = 12), and they were transfected with empty vector and miR-16 overexpression virus, respectively. The lung tissues were extracted in both groups, and then the expression levels of miR-16 and NF-κB were detected via fluorescence quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the association between their expressions was analyzed via Pearson correlation analysis. Moreover, the morphological changes in lung tissues were detected via hematoxylin-eosin (HE) staining, and the differences in the wet/dry weight (W/D) ratio and the pathomorphological score of lung tissues were compared between the two groups. The expression level of NF-κB was detected via immunohistochemistry (IHC) and Western blotting. Our results showed that, there were different degrees of lung injury in lung tissues in both groups. In miR-16 overexpression group, the W/D ratio was significantly higher than that in NC group (P < 0.05), and the pathomorphological score was also significantly higher than that in NC group (P < 0.05). The results of RT-PCR revealed that the mRNA levels of miR-16 and NF-κB in miR-16 overexpression group were 2.5 and 3.7 times higher than those in NC group. The results of Western blotting and IHC also showed that the activity of NF-κB in lung tissues was evidently enhanced in miR-16 overexpression group compared with that in NC group. According to the Pearson correlation analysis, there was a significant positive correlation between the mRNA levels of miR-16 and NF-κB in lung tissues (r = 0.705, P = 0.012). In conclusion, miR-16 activates the NF-κB pathway to initiate a series of inflammatory responses, thereby contributing to the occurrence of ALI in rats.

scholarly journals Recovery from acute lung injury can be regulated via modulation of regulatory T cells and Th17 cells

2018 ◽  
Vol 88 (5) ◽  
pp. e12715 ◽  
Author(s):  
Linlin Wang ◽  
Xiaocen Wang ◽  
Lin Tong ◽  
Jian Wang ◽  
Maosen Dou ◽  
...  
Keyword(s):  
T Cells ◽  
Acute Lung Injury ◽  
Regulatory T Cells ◽  
Lung Injury ◽  
Th17 Cells

scholarly journals Increased Frequencies of Th22 Cells as well as Th17 Cells in the Peripheral Blood of Patients with Ankylosing Spondylitis and Rheumatoid Arthritis

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e31000 ◽  
Author(s):  
Lei Zhang ◽  
Yong-gang Li ◽  
Yu-hua Li ◽  
Lei Qi ◽  
Xin-guang Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Th22 Cells

scholarly journals Baseline Interleukin-6 Level Predicts Risk of Severe COVID-19: A Two-Center, Retrospective Study

2020 ◽  
Author(s):  
Weigang Zhu ◽  
Yingping Wu ◽  
Yueping Liu ◽  
Bin Li ◽  
Pian Xiong ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Characteristic Curve ◽  
Severe Disease ◽  
Multivariate Logistic Regression Analysis ◽  
Area Under The Curve ◽  
Severe Illness ◽  
Mild Disease ◽  
Predictive Variables ◽  
Initial Symptoms ◽  
Quartile Group

Abstract Background:The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has affected almost every country. Interleukin-6 (IL-6), a cytokine secreted by CD4+ T cell, has been shown to be a reliable marker of disease severity and a useful parameter for monitoring progression of coronavirus disease-2019 (COVID-19). However its value as a predictor of severe disease has not been assessed.Methods:A total of 160 laboratory-confirmed COVID-19 patients admitted to two hospitals were enrolledand separated into two groups according to whether or not they progressed to develop severe illness. Demographic and clinical characteristics at admission were compared between the groups.Results: Patients who developed severe COVID-19 had significantly higher baseline IL-6 levels than patients who had mild disease course in hospital (P< 0.001). Patients were further grouped according to quartiles of IL-6 level. The cumulative incidence of severe illnesswas significantly higher in the third and fourth quartiles groups than in the first quartile group (55% vs. 15% and 80% vs. 15%, respectively;bothP< 0.001). In multivariate logistic regression analysis, the risk for developing severe disease was markedly higher in the highest IL-6 quartile than in the lowest quartile (odds ratio: 14.95; 95% confidence interval: 3.65–61.30; P< 0.001). Receiver operating characteristic curve analysis of potential predictive variables showed the area under the curve to be largest for baseline IL-6, with the value of 5.20 pg/mL having the best balance of sensitivity and specificity for predicting risk of severe COVID-19.Conclusion: Serum baseline IL-6 appears to be a reliable predictor of risk of severe COVID-19. Early intervention may be advisable in patients with serum IL-6 levels >5.20 pg/mL, even if initial symptoms are mild.

scholarly journals Electroencephalogram-derived pain index for evaluating pain during labor

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12714
Author(s):  
Liang Sun ◽  
Hong Zhang ◽  
Qiaoyu Han ◽  
Yi Feng
Keyword(s):  
Correlation Analysis ◽  
Rating Scale ◽  
Numerical Rating Scale ◽  
Point Of Care ◽  
Pearson Correlation ◽  
Area Under The Curve ◽  
Labor Pain ◽  
Youden Index ◽  
Discriminative Ability ◽  
Pearson Correlation Analysis

Background The discriminative ability of a point-of-care electroencephalogram (EEG)-derived pain index (Pi) for objectively assessing pain has been validated in chronic pain patients. The current study aimed to determine its feasibility in assessing labor pain in an obstetric setting. Methods Parturients were enrolled from the delivery room at the department of obstetrics in a tertiary hospital between February and June of 2018. Pi values and relevant numerical rating scale (NRS) scores were collected at different stages of labor in the presence or absence of epidural analgesia. The correlation between Pi values and NRS scores was analyzed using the Pearson correlation analysis. The receiver operating characteristic (ROC) curve was plotted to estimate the discriminative capability of Pi to detect labor pain in parturients. Results Eighty paturients were eligible for inclusion. The Pearson correlation analysis exhibited a positive correlation between Pi values and NRS scores in parturients (r = 0.768, P < 0.001). The ROC analysis revealed a cut-off Pi value of 18.37 to discriminate between mild and moderate-to-severe labor pain in parturients. Further analysis indicated that Pi values had the best diagnostic accuracy reflected by the highest area under the curve (AUC) of 0.857, with a sensitivity and specificity of 0.767 and 0.833, respectively, and a Youden index of 0.6. Subgroup analyses further substantiated the correlations between Pi values and NRS scores, especially in parturients with higher pain intensity. Conclusion This study indicates that Pi values derived from EEGs significantly correlate with the NRS scores, and can serve as a way to quantitatively and objectively evaluate labor pain in parturients.

Downregulation of blood and bone marrow neutrophils decreases expression of acute lung injury in sheep

1992 ◽  
Vol 263 (5) ◽  
pp. H1492-H1498
Author(s):  
P. J. McKenna ◽  
D. L. Rosolia ◽  
Y. Ishihara ◽  
K. H. Albertine ◽  
N. C. Staub ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Peripheral Blood ◽  
Pulmonary Circulation ◽  
Tissue Injury ◽  
Blood Neutrophils ◽  
Superoxide Release

We have shown that infusion of zymosan-activated plasma (ZAP) in sheep causes acute lung injury and downregulates peripheral blood neutrophils in that elicited superoxide release is reduced for at least 24 h after the infusion. The present study was designed to test the following hypotheses: 1) peripheral blood neutrophils are representative of neutrophils marginated in the pulmonary circulation, 2) blood neutrophils are downregulated because neutrophils developing in bone marrow are similarly affected, and 3) downregulated neutrophils have a reduced capacity to produce tissue injury. In a series of experiments in 21 sheep, we showed that elicited superoxide release was similar in peripheral blood neutrophils and in marginated neutrophils washed out of the pulmonary vascular bed. Measurements of superoxide release from blood and bone marrow neutrophils collected 2-24 h after ZAP infusion revealed progressive downregulation with time and greater downregulation of superoxide release in bone marrow neutrophils compared with peripheral blood neutrophils. Finally, after downregulating peripheral blood neutrophils, subsequent infusion of ZAP in conscious sheep produced sequestration of neutrophils in the pulmonary circulation but failed to produce a sustained increase in lung lymph protein clearance. The results suggest that neutrophil downregulation, as measured in vitro, is expressed in vivo as reduced ability of neutrophils to produce tissue injury when challenged by an activating agent.

Diagnostic and prognostic roles of peripheral blood Toll-like receptor-4 and stanniocalcin-1 genes expression in acute lung injury

Immunobiology ◽  
2019 ◽  
Vol 224 (6) ◽  
pp. 734-738
Author(s):  
Marwa M. Esawy ◽  
Marwa A. Shabana ◽  
Shereen A. Baioumy ◽  
Nagwan A. Ismail
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Peripheral Blood ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Genes Expression

scholarly journals Association between peripheral blood mononuclear cell ORMDL3 expression and the asthma predictive index in preschool children

2019 ◽  
Vol 47 (8) ◽  
pp. 3727-3736 ◽  
Author(s):  
Yaqin Li ◽  
Lanfang Cao ◽  
Qing Yu ◽  
Haiyan Xue ◽  
Yanming Lu
Keyword(s):  
Risk Factors ◽  
Preschool Children ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Immunoglobulin E ◽  
Characteristic Curve ◽  
Area Under The Curve ◽  
House Dust Mites ◽  
Predictive Index ◽  
Peripheral Blood Mononuclear

Objective This study aimed to assess whether orosomucoid 1-like 3 (ORMDL3) expression and environmental and clinical factors are associated with wheezing episodes in preschool children. Methods Children diagnosed with wheezing episodes were classified according to their asthma predictive index (API) in the past year as follows: API+ (≥4 wheezing episodes), API− (1–3 wheezing episodes), and API0 (without wheezing). ORMDL3 expression was assessed by real-time polymerase chain reaction in peripheral blood mononuclear cells (PBMCs). Receiver operating characteristic curve analysis of ORMDL3 expression and the API was performed for diagnosing wheezing episodes. Correlations between ORMDL3 expression and asthma risk factors were examined using Spearman’s correlation. Results PBMC ORMDL3 expression was higher in the API+ group compared with the API− and API0 groups. The area under the curve for ORMDL3 expression was 0.820 (95% confidence interval, 0.771–0.869). ORMDL3 expression was positively correlated with the API ( r = 0.447), infantile eczema ( r = 0.499), wheezing ( r = 0.516), total immunoglobulin E ( r = 0.208), and environmental factors, including Dermatophagoides pteronyssinus ( r = 0.357), house dust mites ( r = 0.112), dog fur ( r = 0.226), and Aspergillus( r = 0.257). ORMDL3 expression was negatively correlated with amaranth ( r = −0.122). Conclusions ORMDL3 expression in PBMCs is positively associated with the API and some asthma-related clinical and environmental risk factors in preschoolers.

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