scholarly journals Transcriptome Analysis and Screening for Potential Target Genes for RNAi-Mediated Pest Control of the Beet Armyworm, Spodoptera exigua

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65931 ◽  
Author(s):  
Hang Li ◽  
Weihua Jiang ◽  
Zan Zhang ◽  
Yanru Xing ◽  
Fei Li
Keyword(s):  
Pest Control ◽  
Transcriptome Analysis ◽  
Spodoptera Exigua ◽  
Target Genes ◽  
Beet Armyworm ◽  
Potential Target

scholarly journals Genome and transcriptome analysis of the beet armyworm Spodoptera exigua reveals targets for pest control

2021 ◽  
Author(s):  
Sabrina Simon ◽  
Thijmen Breeschoten ◽  
Hans J Jansen ◽  
Ron P Dirks ◽  
M. Eric Schranz ◽  
...  
Keyword(s):  
Pest Control ◽  
Genome Assembly ◽  
Spodoptera Exigua ◽  
Control Strategies ◽  
Beet Armyworm ◽  
Cultivated Plants ◽  
Comparative Genomic ◽  
Supporting Evidence ◽  
Pest Species ◽  
Hybrid Genome

Background: The genus Spodoptera (Lepidoptera: Noctuidae) includes some of the most infamous insect pests of cultivated plants including Spodoptera frugiperda, Spodoptera litura and Spodoptera exigua. To effectively develop targeted pest control strategies for diverse Spodoptera species, genomic resources are highly desired. To this aim, we provide the genome assembly and developmental transcriptome comprising all major life stages of S. exigua, the beet armyworm. Spodoptera exigua is a polyphagous herbivore that can feed from > 130 host plants including several economically important crops. Results: The 419 Mb beet armyworm genome was sequenced from a female S. exigua pupa. Using a hybrid genome sequencing approach (Nanopore long read data and Illumina short read), a high-quality genome assembly was achieved (N50=1.1 Mb). An official gene set (OGS, 18,477 transcripts) was generated by automatic annotation and by using transcriptomic RNA-seq data sets of 18 S. exigua samples as supporting evidence. In-depth analyses of developmental stage-specific expression in combination with gene tree analyses of identified homologous genes across Lepidoptera genomes revealed potential Spodoptera-specific genes of interest such as mg7 and REPAT46 upregulated during 1st and 3rd instar larval stages for targeted pest-outbreak management. Conclusions: The beet armyworm genome sequence and developmental transcriptome covering all major developmental stages provides critical insights into the biology of this devastating polyphagous insect pest species with a worldwide distribution. In addition, comparative genomic analyses across Lepidoptera significantly advance our knowledge to further control other invasive Spodoptera species and reveals potential lineage-specific target genes for pest control strategies.

Disruption of Spodoptera exigua larval development by silencing chitin synthase gene A with RNA interference

2008 ◽  
Vol 98 (6) ◽  
pp. 613-619 ◽  
Author(s):  
X. Chen ◽  
H. Tian ◽  
L. Zou ◽  
B. Tang ◽  
J. Hu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Pest Control ◽  
Trichoplusia Ni ◽  
Spodoptera Exigua ◽  
Control Method ◽  
Insect Cell Line ◽  
Chitin Synthase ◽  
Beet Armyworm

AbstractRNA interference (RNAi) is a powerful tool for rapidly analyzing gene functions. However, little is known about the possible use of dsRNA/siRNA as a pest control method. Here, we demonstrate that dsRNA/siRNA can induce the silence of chitin synthase gene A (CHSA), which is an important gene for the growth and development of cuticles and trachea in beet armyworm, Spodoptera exigua. Based on the in vitro RNAi experiments in an insect cell line (Trichoplusia ni High 5), in vivo RNAi was performed by injecting synthesized dsRNA/siRNA into the 4th instar larvae of S. exigua. Significantly lower levels of CHSA transcripts were detected. In addition, the cuticle of these insects was disordered and the epithelial walls of larval trachea did not expand uniformly in injected individuals. Moreover, Injections significantly increased abnormalities relative to control larvae. These results highlighted the possibility of dsRNA/siRNA for gene function studies in lepidopteran insects and future pest control.

Transcriptome analysis of the ovary of beet armyworm Spodoptera exigua under different exposures of cadmium stress

Chemosphere ◽  
2020 ◽  
Vol 251 ◽  
pp. 126372 ◽  
Author(s):  
Honghua Su ◽  
Yang Yang ◽  
Jincheng Zou ◽  
Yuqing Cheng ◽  
Yong Yang ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Spodoptera Exigua ◽  
Cadmium Stress ◽  
Beet Armyworm

scholarly journals Genome and transcriptome analysis of the beet armyworm Spodoptera exigua reveals targets for pest control

2021 ◽  
Author(s):  
Sabrina Simon ◽  
Thijmen Breeschoten ◽  
Hans J Jansen ◽  
Ron P Dirks ◽  
M Eric Schranz ◽  
...  
Keyword(s):  
Pest Control ◽  
Genome Assembly ◽  
Spodoptera Exigua ◽  
Control Strategies ◽  
Beet Armyworm ◽  
Cultivated Plants ◽  
Comparative Genomic ◽  
Supporting Evidence ◽  
Pest Species ◽  
Hybrid Genome

Abstract The genus Spodoptera (Lepidoptera: Noctuidae) includes some of the most infamous insect pests of cultivated plants including Spodoptera frugiperda, Spodoptera litura and Spodoptera exigua. To effectively develop targeted pest control strategies for diverse Spodoptera species, genomic resources are highly desired. To this aim, we provide the genome assembly and developmental transcriptome comprising all major life stages of S. exigua, the beet armyworm. Spodoptera exigua is a polyphagous herbivore that can feed on > 130 host plants, including several economically important crops. The 419 Mb beet armyworm genome was sequenced from a female S. exigua pupa. Using a hybrid genome sequencing approach (Nanopore long read data and Illumina short read), a high-quality genome assembly was achieved (N50=1.1 Mb). An official gene set (OGS, 18,477 transcripts) was generated by automatic annotation and by using transcriptomic RNA-seq data sets of 18 S. exigua samples as supporting evidence. In-depth analyses of developmental stage-specific expression combined with gene tree analyses of identified homologous genes across Lepidoptera genomes revealed four potential genes of interest (three of them Spodoptera-specific) upregulated during 1st and 3rd instar larval stages for targeted pest-outbreak management. The beet armyworm genome sequence and developmental transcriptome covering all major developmental stages provide critical insights into the biology of this devastating polyphagous insect pest species worldwide. In addition, comparative genomic analyses across Lepidoptera significantly advance our knowledge to further control other invasive Spodoptera species and reveals potential lineage-specific target genes for pest control strategies.

scholarly journals Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant Saccharomyces cerevisiae

2021 ◽  
Vol 9 (2) ◽  
pp. 233
Author(s):  
Buli Su ◽  
Anzhang Li ◽  
Ming-Rong Deng ◽  
Honghui Zhu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptome Analysis ◽  
Gene Expression Analysis ◽  
Target Genes ◽  
Copper Ions ◽  
Carotenoid Production ◽  
Carotenoid Accumulation ◽  
Nutritional Components ◽  
Differential Gene ◽  
First Time

We previously constructed a Saccharomyces cerevisiae carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that ADY2, HES1, and CUP1 showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO4 in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of ACE1. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered S. cerevisiae and provided a new target ACE1 for genetic engineering of S. cerevisiae for the bioproduction of carotenoids.

scholarly journals Caterpillar salivary enzymes: "eliciting" a response

2004 ◽  
Vol 85 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Magali Merkx-Jacques ◽  
Jacqueline C. Bede
Keyword(s):  
Artificial Diet ◽  
Spodoptera Exigua ◽  
Induced Defense ◽  
Defense Responses ◽  
Beet Armyworm ◽  
Herbivorous Insect ◽  
Plant Responses ◽  
Plant Defense Responses ◽  
Induced Defense Responses ◽  
Bertha Armyworm

Abstract Plants exhibit remarkable plasticity in their ability to differentiate between herbivorous insect species and subtly adjust their defense responses to target distinct pests. One key mechanism used by plants to recognize herbivorous caterpillars is elicitors present in their oral secretions; however, these elicitors not only cause the induction of plant defenses but recent evidence suggests that they may also suppress plant responses. The absence of “expected changes” in induced defense responses of insect-infested plants has been attributed to hydrogen peroxide produced by caterpillar salivary glucose oxidase (GOX). Activity of this enzyme is variable among caterpillar species; it was detected in two generalist caterpillars, the beet armyworm (Spodoptera exigua) and the bertha armyworm (Mamestra configurata), but not in other generalist or specialist caterpillar species tested. In the beet armyworm, GOX activity fluctuated over larval development with high activity associated with the salivary glands of fourth instars. Larval salivary GOX activity of the beet armyworm and the bertha armyworm was observed to be significantly higher in caterpillars reared on artificial diet as compared with those reared on Medicago truncatula plants. This implies that a factor in the diet is involved in the regulation of caterpillar salivary enzyme activity. Therefore, plant diet may be regulating caterpillar oral elicitors that are involved in the regulation of plant defense responses: our goal is to understand these two processes.

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