scholarly journals Human Bladder Uroepithelial Cells Synergize with Monocytes to Promote IL-10 Synthesis and Other Cytokine Responses to Uropathogenic Escherichia coli

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78013 ◽  
Author(s):  
Benjamin L. Duell ◽  
Alison J. Carey ◽  
Samantha J. Dando ◽  
Mark A. Schembri ◽  
Glen C. Ulett
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Human Bladder ◽  
Cytokine Responses ◽  
Uroepithelial Cells

scholarly journals Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells

2006 ◽  
Vol 74 (1) ◽  
pp. 750-757 ◽  
Author(s):  
Yarery C. Smith ◽  
Kerian K. Grande ◽  
Susan B. Rasmussen ◽  
Alison D. O'Brien
Keyword(s):  
Escherichia Coli ◽  
Three Dimensional ◽  
Uropathogenic Escherichia Coli ◽  
Urothelial Cells ◽  
Human Bladder ◽  
Upec Strain ◽  
Tissue Specific ◽  
Microgravity Conditions ◽  
Uroepithelial Cells

ABSTRACT Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

scholarly journals Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 272
Author(s):  
Wenbo Yang ◽  
Peng Liu ◽  
Ying Chen ◽  
Qingyu Lv ◽  
Zhongtian Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Uropathogenic Escherichia Coli ◽  
Transmission Electron ◽  
Promising Target ◽  
Bacterial Morphology ◽  
Important Virulence Factor ◽  
Uroepithelial Cells

Uropathogenic Escherichia coli (UPEC) is the most common pathogenic bacteria associated with urinary tract infection (UTI). UPEC can cause UTI by adhering to and invading uroepithelial cells. Fimbriae is the most important virulence factor of UPEC, and a potentially promising target in developing novel antibacterial treatments. In this study, the antibacterial properties and effects of the compound dictamnine, extracted from the traditional Chinese medicine Cortex Dictamni, on the bacterial morphology, cell adhesion, and invasion of UPEC were studied. Dictamnine exhibited no obvious antibacterial activity against UPEC, but significantly impeded the ability of UPEC to adhere to and invade uroepithelial cells. RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes, and also downregulated adhesion-related receptor genes of uroepithelial cells. Transmission electron microscopy showed that dictamnine destroyed the structure of the fimbriae and the surface of the bacteria became smooth. These results suggest that dictamnine may help to prevent UTI by simultaneously targeting UPEC fimbriae and urothelial adhesin receptors, and may have a potential use as a new anti-UPEC drug.

Distribution of fimH Gene in Local Isolates of Adhesive Uropathogenic Escherichia coli

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Omar Abdulkareem Ali ◽  
Ruqayah Qubtan Taha
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Uropathogenic Escherichia Coli ◽  
Adhesion Assay ◽  
E Coli ◽  
Type 1 Fimbriae ◽  
Formation Type ◽  
Uroepithelial Cells

Adhesion is an influential step for bacterial vigor in clinical micro-environments, type 1 fimbriae are essential virulence factors help uropathogenic E. coli in invasion and colonization of uroepithelial cells, the first step of UTIs and biofilm formation. Type 1 fimbriae of E. coli contain FimH protein at the tip encoding via fimH gene cluster, this study was conducted for determination the fimH gene distribution in uro-pathogenic E. coli isolated from UTIs patients. The results of adhesion assay show that (83.6%) of uropathogenic E. coli were high adherent isolates. While the results of E. coli fimH gene amplification prove that, of all E. coli isolates, the fimH gene was found in (87.1%), while among high adherent isolates it was found in (92.6%), and that Shows the function of type 1 fimbriae in the colonization and infection of urinary tracts in addition to other adhesions virulence agents of uropathogenic E. coli.

scholarly journals Biological and genetic characteristics of uropathogenic Escherichia coli strains

2001 ◽  
Vol 43 (6) ◽  
pp. 303-310 ◽  
Author(s):  
Wanderley Dias da SILVEIRA ◽  
Fabiane BENETTI ◽  
Marcelo LANCELLOTTI ◽  
Alessandra FERREIRA ◽  
Vera Nisaka SOLFERINI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hela Cells ◽  
Fragment Length Polymorphism ◽  
Clinical Signs ◽  
Uropathogenic Escherichia Coli ◽  
Enzyme Electrophoresis ◽  
Enterobacterial Repetitive Intergenic Consensus ◽  
Specific Primers ◽  
Genetic Characteristics ◽  
Uroepithelial Cells

The aim of the present study was to determine biological characteristics such as expression of fimbriae, Congo red binding, production of hemolysin and aerobactin, adhesion to HeLa and uroepithelial cells and invasion of HeLa cells by Escherichia coli isolates obtained from patients showing clinical signs of urinary tract infection (UTI). Also, the presence of genes (apa, afa, spa) for fimbria expression and cytotoxic necrotizing factors (CNF1, CNF2) was assayed using specific primers in PCR. The data obtained were compared with the clonal relationships obtained by analysis of multilocus enzyme electrophoresis (MLEE), restriction fragment length polymorphism (RFLP) of the rDNA (ribotyping) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). All isolates but one presented a combination of at least two of the characteristics studied, a fact suggesting the presence of pathogenicity islands (PAIs). Diffuse adherence type to HeLa cells was observed to occur in most of the strains, but adhesion to uroepithelial cells seems to be a more reliable test to verify pathogenicity. Although four strains seemed to be able to invade HeLa cells when assayed by light microscopy, electron microscopy studies demonstrated that these strains were not invasive. MLEE, RFLP and ERIC-PCR were able to group the isolates differently into main clusters that were not correlated with the presence of pathogenic traits.

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