Extract of maydis stigma (Zea mays L.) inhibits the adhesion of uropathogenic Escherichia coli (UPEC) to human bladder cells

ABSTRACT Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

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Comprehensive Identification of Fim-Mediated Inversions in Uropathogenic Escherichia coli with Structural Variation Detection Using Relative Entropy

10.1101/494401 ◽

2018 ◽

Author(s):

Colin W. Russell ◽

Rashmi Sukumaran ◽

Lu Ting Liow ◽

Balamurugan Periaswamy ◽

Shazmina Rafee ◽

...

Keyword(s):

Escherichia Coli ◽

Relative Entropy ◽

Structural Variation ◽

Infection Type ◽

Uropathogenic Escherichia Coli ◽

Structural Variations ◽

Type 1 Pili ◽

Bladder Cells ◽

Tract Infections

Most urinary tract infections (UTIs) are caused by uropathogenic Escherichia coli (UPEC), which depend on an extracellular organelle (Type 1 pili) for adherence to bladder cells during infection. Type 1 pilus expression is partially regulated by inversion of a piece of DNA referred to as fimS, which contains the promoter for the fim operon encoding Type 1 pili. fimS inversion is regulated by up to five recombinases collectively known as Fim recombinases. These Fim recombinases are currently known to regulate two other switches: the ipuS and hyxS switches. A long-standing question has been whether the Fim recombinases regulate the inversion of other switches, perhaps to coordinate expression for adhesion or virulence. We answered this question using whole genome sequencing with a newly developed algorithm (Structural Variation detection using Relative Entropy, SVRE) for calling structural variations using paired-end short read sequencing. SVRE identified all of the previously known switches, refining the specificity of which recombinases act at which switches. Strikingly, we found no new inversions that were mediated by the Fim recombinases. We conclude that the Fim recombinases are each highly specific for a small number of switches. We hypothesize that the unlinked Fim recombinases have been recruited to regulate fimS, and fimS only, as a secondary locus; this further implies that regulation of Type 1 pilus expression (and its role in gastrointestinal and/or genitourinary colonization) is important enough, on its own, to influence the evolution and maintenance of multiple additional genes within the accessory genome of E. coli.

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Pectic Oligosaccharides from Cranberry Prevent Quiescence and Persistence in the Uropathogenic Escherichia coli CFT073

Scientific Reports ◽

10.1038/s41598-019-56005-w ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Jiadong Sun ◽

Robert W. Deering ◽

Zhiyuan Peng ◽

Laila Najia ◽

Christina Khoo ◽

...

Keyword(s):

Escherichia Coli ◽

Chemical Compounds ◽

Uropathogenic Escherichia Coli ◽

Cranberry Juice ◽

Persister Cells ◽

E Coli ◽

Pectic Oligosaccharides ◽

Bladder Cells ◽

Strain Cft073 ◽

Tract Infections

AbstractUrinary tract infections (UTIs) caused by Escherichia coli create a large burden on healthcare and frequently lead to recurrent infections. Part of the success of E. coli as an uropathogenic bacterium can be attributed to its ability to form quiescent intracellular reservoirs in bladder cells and its persistence after antibiotic treatment. Cranberry juice and related products have been used for the prevention of UTIs with varying degrees of success. In this study, a group of cranberry pectic oligosaccharides (cPOS) were found to both inhibit quiescence and reduce the population of persister cells formed by the uropathogenic strain, CFT073. This is the first report detailing constituents of cranberry with the ability to modulate these important physiological aspects of uropathogenic E. coli. Further studies investigating cranberry should be keen to include oligosaccharides as part of the ‘active’ cocktail of chemical compounds.

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Escherichia coli from retail meats carry genes associated with uropathogenic Escherichia coli, but are weakly invasive in human bladder cell culture

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2011.04978.x ◽

2011 ◽

Vol 110 (5) ◽

pp. 1166-1176 ◽

Cited By ~ 8

Author(s):

X. Xia ◽

J. Meng ◽

P.F. McDermott ◽

S. Zhao

Keyword(s):

Escherichia Coli ◽

Cell Culture ◽

Uropathogenic Escherichia Coli ◽

Human Bladder ◽

Bladder Cell ◽

Retail Meats

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Aqueous Root Extract from Ononis spinosa Exerts Anti-adhesive Activity against Uropathogenic Escherichia coli

Planta Medica ◽

10.1055/a-1089-8645 ◽

2020 ◽

Vol 86 (04) ◽

pp. 247-254 ◽

Cited By ~ 2

Author(s):

Melanie Deipenbrock ◽

Jandirk Sendker ◽

Andreas Hensel

Keyword(s):

Escherichia Coli ◽

Urinary Tract ◽

Cell Viability ◽

Urinary Tract Infections ◽

Bacterial Adhesion ◽

Uropathogenic Escherichia Coli ◽

E Coli ◽

T24 Cells ◽

Bladder Cells ◽

Tract Infections

AbstractExtracts from Ononis spinosa are traditionally used for urinary tract infections due to diuretic and anti-inflammatory activity. A potential influence on the virulence of uropathogenic Escherichia coli has not been investigated until now. The following study aimed to investigate the influence of an aqueous O. spinosa extract on uropathogenic E. coli and uropathogenic E. coli host cell interaction. A hot water extract from the roots of O. spinosa (O. spinosa extract) was characterized by LC-qTOF-MS. The influence of O. spinosa extract on the proliferation of uropathogenic E. coli UTI89 and on cell viability against human T24 bladder cells was investigated. Anti-adhesive activity of O. spinosa extract was assessed by flow cytometry, evaluating the adhesion of fluorescent-labelled UTI89 to T24 bladder cells. Internalization of uropathogenic E. coli into T24 cells was monitored by an invasion assay. O. spinosa extract was characterized by the presence of isoflavones, isoflavanones, licoagrosides, pterocarpans, tartaric acid derivatives, and saponines. O. spinosa extract had no influence on the proliferation of uropathogenic E. coli (125 – 1000 µg/mL) and did not influence the cell viability of T24 cells. Bacterial adhesion to T24 cells was significantly (p > 0.001) inhibited by O. spinosa extract in a concentration-dependent manner (125 – 1000 µg/mL) during coincubation. Preincubation of uropathogenic E. coli or T24 cells with O. spinosa extract reduced bacterial adhesion, but to a lower extent than during coincubation. Consequently, the reduced bacterial adhesion also leads to a reduced internalization of uropathogenic E. coli uropathogenic E. coli into the host cell. O. spinosa extract does not interact with FimH-mediated uropathogenic E. coli adhesion to host cells. From these data, the traditional use of O. spinosa extracts for urinary tract infections seems to be rationalized.

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Decolorization and detoxification of batik dye effluent containing Indigosol Blue-04B using fungi isolated from contaminated dye effluent

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.32332 ◽

2018 ◽

Vol 23 (2) ◽

pp. 54 ◽

Cited By ~ 3

Author(s):

Ratna Stia Dewi ◽

Rina Sri Kasiamdari ◽

Erni Martani ◽

Yekti Asih Purwestri

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Zea Mays ◽

Soil Bacteria ◽

Room Temperature ◽

Zea Mays L ◽

Inhibition Zone ◽

Gram Negative ◽

Central Java ◽

Aspergillus Sp

Fungi are capable of treating various synthetic dye effluents. Previously, we isolated seven strains of fungi from contaminated batik dye effluent at Banyumas, Central Java. The aims of this study were to screen the ability of these fungi to decolorize batik dye effluents containing Indigosol Blue-04B and to investigate the phytotoxicity effects of biodegraded effluent on the germination of corn seeds Zea mays L. and green bean seeds Vigna radiata (L.) Wilczek. In addition, the decolorized effluents were tested for toxic effect on the agriculturally important gram-positive and gram-negative soil bacteria Bacillus cereus and Azotobacter sp., Staphylococcus aureus and Escherichia coli, respectively. Study of decolorization showed that fungi were able to decolorize Indigosol Blue-04B batik dye effluents by 21.04% to 99.89% at room temperature after three days of incubation. The assay of phytotoxicity showed that both plumule and radicle length of Z. mays and V. radiata grown on the decolorized effluent was longer than on untreated effluent. The percentage of Z. mays and V. radiata seed germination in decolorized effluent was higher than in untreated effluent. There was no inhibition zone found around the decolorized effluent samples after incubating the bacteria for 48 hours. Aspergillus sp. 3 was the most effective for degradation and could be used for batik effluent mycoremediation processes.

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Maize (Zea mays L.) DNA sequences homologous to the oncogenes myb, ras, and src

Genome ◽

10.1139/g88-132 ◽

1988 ◽

Vol 30 (5) ◽

pp. 820-824 ◽

Cited By ~ 5

Author(s):

R. B. Zabulionis ◽

B. G. Atkinson ◽

J. D. Procunier ◽

D. B. Walden

Keyword(s):

Escherichia Coli ◽

Zea Mays ◽

Cell Division ◽

Dna Sequences ◽

Zea Mays L ◽

Genomic Sequence ◽

Evolutionary Conservation ◽

Higher Plant ◽

E Coli ◽

Homologous Sequences

The presence of genes that may govern cell division and differentiation is being investigated in the agronomically important higher plant Zea mays. Heterologous animal oncogene probes (v-myb, v-fos, v-src, and v-Ki-ras) were hybridized to Southern-blotted endonuclease-restricted fragments of maize DNA under conditions that allowed up to 28% mismatch between the probe and genomic sequence. Human, yeast, and Escherichia coli endonuclease restricted DNA served as controls for the hybridization conditions used. The Southern blotted DNAs were hybridized with probes to ribosomal DNA and pBR322 to ensure that the observed hybridization signals were not due to spurious binding or contamination of the oncogene probes. Maize DNA sequences homologous to v-myb, v-src, and v-Ki-ras were detected. No maize sequences homologous to the v-fos probe were detected. The oncogene probes did not detect any homologous sequences in E. coli DNA and all the reported homologous bands in human and yeast DNA were observed. These results illustrate the evolutionary conservation of animal proto-oncogenes within the plant kingdom, and suggest that these sequences may play a role in the replication and differentiation of plant cells.Key words: oncogenes, v-myb, v-fos, v-src, v-Ki-ras, Zea mays.

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