scholarly journals Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells

2016 ◽  
Vol 131 ◽  
pp. 177-189 ◽  
Author(s):  
Daniël J. Wurpel ◽  
Makrina Totsika ◽  
Luke P. Allsopp ◽  
Richard I. Webb ◽  
Danilo G. Moriel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Urine ◽  
Biofilm Formation ◽  
Comparative Proteomics ◽  
Uropathogenic Escherichia Coli ◽  
Uroepithelial Cells

Distribution of fimH Gene in Local Isolates of Adhesive Uropathogenic Escherichia coli

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Omar Abdulkareem Ali ◽  
Ruqayah Qubtan Taha
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Uropathogenic Escherichia Coli ◽  
Adhesion Assay ◽  
E Coli ◽  
Type 1 Fimbriae ◽  
Formation Type ◽  
Uroepithelial Cells

Adhesion is an influential step for bacterial vigor in clinical micro-environments, type 1 fimbriae are essential virulence factors help uropathogenic E. coli in invasion and colonization of uroepithelial cells, the first step of UTIs and biofilm formation. Type 1 fimbriae of E. coli contain FimH protein at the tip encoding via fimH gene cluster, this study was conducted for determination the fimH gene distribution in uro-pathogenic E. coli isolated from UTIs patients. The results of adhesion assay show that (83.6%) of uropathogenic E. coli were high adherent isolates. While the results of E. coli fimH gene amplification prove that, of all E. coli isolates, the fimH gene was found in (87.1%), while among high adherent isolates it was found in (92.6%), and that Shows the function of type 1 fimbriae in the colonization and infection of urinary tracts in addition to other adhesions virulence agents of uropathogenic E. coli.

scholarly journals Uropathogenic Escherichia coli Biofilm-Forming Capabilities are not Predictable from Clinical Details or from Colonial Morphology

Diseases ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 11 ◽  
Author(s):  
Shane Whelan ◽  
Mary Claire O’Grady ◽  
Dan Corcoran ◽  
Karen Finn ◽  
Brigid Lucey
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Culture Medium ◽  
Recurrent Infection ◽  
Positive Control ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Recurrent Uti ◽  
Colonial Morphology ◽  
The Difference

Antibiotic resistance is increasing to an extent where efficacy is not guaranteed when treating infection. Biofilm formation has been shown to complicate treatment, whereby the formation of biofilm is associated with higher minimum inhibitory concentration values of antibiotic. The objective of the current paper was to determine whether biofilm formation is variable among uropathogenic Escherichia coli isolates and whether formation is associated with recurrent urinary tract infection (UTI), and whether it can be predicted by phenotypic appearance on culture medium A total of 62 E. coli isolates that were reported as the causative agent of UTI were studied (33 from patients denoted as having recurrent UTI and 29 from patients not specified as having recurrent UTI). The biofilm forming capability was determined using a standard microtitre plate method, using E. coli ATCC 25922 as the positive control. The majority of isolates (93.6%) were found to be biofilm formers, whereby 81% were denoted as strong or very strong producers of biofilm when compared to the positive control. Through the use of a Wilcox test, the difference in biofilm forming propensity between the two patient populations was found to not be statistically significant (p = 0.5). Furthermore, it was noted that colony morphology was not a reliable predictor of biofilm-forming propensity. The findings of this study indicate that biofilm formation is very common among uropathogens, and they suggest that the biofilm-forming capability might be considered when treating UTI. Clinical details indicating a recurrent infection were not predictors of biofilm formation.

scholarly journals Distribution of genes encoding adhesins and biofilm formation capacity among Uropathogenic Escherichia coli isolates in relation to the antimicrobial resistance

2020 ◽  
Vol 20 (1) ◽  
pp. 238-247
Author(s):  
Ashraf A Kadry ◽  
Nour M Al-Kashef ◽  
Amira M El-Ganiny
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Critical Role ◽  
Uropathogenic Escherichia Coli ◽  
Diffusion Method ◽  
Esbl Production ◽  
Microtitre Plate Assay ◽  
Genes Encoding

Background: Escherichia coli is the most predominant pathogen involved in UTIs. Mainly, fimbrial surface appendages are impli- cated in adherence to urothelium besides non-fimbrial proteins. Objectives: To determine prevalence of genes encoding fimbrial and non-fimbrial proteins among Uropathogenic Escherichia coli (UPEC). Furthermore, distribution of these genes and biofilm formation capacity were investigated in relation to antimicrobial resistance. Methods: Antimicrobial susceptibility of 112 UPEC isolates was performed using disc diffusion method. ESBL production was confirmed by double disc synergy test. Genes encoding fimbrial and non-fimbrial proteins were detected using PCR and biofilm formation was investigated using microtitre plate assay. Results: UPEC isolates exhibited high resistance against doxycyclines (88.39 %), β-lactams (7.14-86.6%), sulphamethoxaz- ole–trimethoprim (53.75%) and fluoro-quinolones (50%). Fifty percent of tested isolates were ESBL producers. PapGII gene was statistically more prevalent among pyelonephritis isolates. SfaS, focG and picU genes were statistically associated with flu- oro-quinolone (FQs) sensitive isolates and Dr/afaBC gene was statistically associated with ESBL production. Moreover, non- MDR isolates produced sturdier biofilm. Conclusion: PapGII adhesin variant seems to have a critical role in colonization of upper urinary tract. There is a possible link between antimicrobial resistance and virulence being capable of affecting the distribution of some genes besides its negative impact on biofilm formation. Keywords: Urinary tract infection; Escherichia coli; UPEC; adhesin genes; ESBL; biofilm.  

scholarly journals Human Bladder Uroepithelial Cells Synergize with Monocytes to Promote IL-10 Synthesis and Other Cytokine Responses to Uropathogenic Escherichia coli

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78013 ◽  
Author(s):  
Benjamin L. Duell ◽  
Alison J. Carey ◽  
Samantha J. Dando ◽  
Mark A. Schembri ◽  
Glen C. Ulett
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Human Bladder ◽  
Cytokine Responses ◽  
Uroepithelial Cells

scholarly journals Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells

2006 ◽  
Vol 74 (1) ◽  
pp. 750-757 ◽  
Author(s):  
Yarery C. Smith ◽  
Kerian K. Grande ◽  
Susan B. Rasmussen ◽  
Alison D. O'Brien
Keyword(s):  
Escherichia Coli ◽  
Three Dimensional ◽  
Uropathogenic Escherichia Coli ◽  
Urothelial Cells ◽  
Human Bladder ◽  
Upec Strain ◽  
Tissue Specific ◽  
Microgravity Conditions ◽  
Uroepithelial Cells

ABSTRACT Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

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