scholarly journals Impact of Campylobacter jejuni cj0268c Knockout Mutation on Intestinal Colonization, Translocation, and Induction of Immunopathology in Gnotobiotic IL-10 Deficient Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e90148 ◽  
Author(s):  
Markus M. Heimesaat ◽  
Raimond Lugert ◽  
André Fischer ◽  
Marie Alutis ◽  
Anja A. Kühl ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Intestinal Colonization ◽  
Deficient Mice ◽  
Knockout Mutation

scholarly journals A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection

2007 ◽  
Vol 75 (4) ◽  
pp. 1994-2003 ◽  
Author(s):  
Robert O. Watson ◽  
Veronica Novik ◽  
Dirk Hofreuter ◽  
María Lara-Tejero ◽  
Jorge E. Galán
Keyword(s):  
Campylobacter Jejuni ◽  
Virulence Genes ◽  
Potential Role ◽  
Bacterial Pathogen ◽  
Adaptor Protein ◽  
Myeloid Differentiation ◽  
Deficient Mouse ◽  
Immunocompetent Mice ◽  
Myeloid Differentiation Factor ◽  
Deficient Mice

ABSTRACT Campylobacter jejuni is a major worldwide cause of enteric illnesses. Adult immunocompetent mice are not susceptible to C. jejuni infection. However, we show here that mice deficient in the adaptor protein myeloid differentiation factor 88 (MyD88), which is required for signaling through most Toll-like receptors, can be stably colonized by C. jejuni but not by isogenic derivatives carrying mutations in known virulence genes. We also found that Nramp1 deficiency increases the mouse susceptibility to C. jejuni infection when administered systemically. These results indicate that MyD88-deficient mice could be a useful model to study C. jejuni colonization and reveal a potential role for Nramp1 in the control of this bacterial pathogen.

Postchill Campylobacter Prevalence on Broiler Carcasses in Relation to Slaughter Group Colonization Level and Chilling System

2006 ◽  
Vol 69 (3) ◽  
pp. 495-499 ◽  
Author(s):  
M. LINDBLAD ◽  
I. HANSSON ◽  
I. VÅGSHOLM ◽  
R. LINDQVIST
Keyword(s):  
Campylobacter Jejuni ◽  
Surveillance Program ◽  
Intestinal Colonization ◽  
Direct Plating ◽  
Baseline Study ◽  
Group Data ◽  
Low Degree ◽  
High Degree ◽  
Campylobacter Spp ◽  
Skin Samples

Data from an ongoing national surveillance program of Campylobacter prevalence in broiler slaughter groups were related to results from a 1-year baseline study of broiler carcasses postchill. The goals were to establish the relation between Campylobacter prevalence in slaughter groups and on carcasses and to determine the effect of various chilling systems on Campylobacter prevalence. Pooled cloacal and neck skin samples from the surveillance program were analyzed after enrichment. Carcass rinse samples from the baseline study were analyzed after enrichment and by direct plating. Data from both studies were available for 614 carcasses. Direct-plating analyses indicated that the percentages of carcasses positive for Campylobacter jejuni and other Campylobacter spp. in slaughter groups with negative cloacal samples were 2 and 10%, respectively, whereas enrichment analyses indicated prevalences of 2% in both cases. Campylobacter prevalence in slaughter groups with a high degree of intestinal colonization (more than half of the pooled cloacal samples positive) was significantly higher than in slaughter groups with a low degree of colonization (76 to 85% and 30 to 50%, respectively, depending on Campylobacter spp. and analytical method). The prevalence of Campylobacter-positive carcasses postchill was at the same level as the prevalence of carcasses that originated from slaughter groups with positive neck skin samples at four of the six slaughterhouses. Only at one slaughterhouse, with an air-chilling system, was the postchill prevalence (13%) lower than that expected from slaughter group data (23%). The postchill prevalence (43%) was higher than that expected from slaughter group data (33%) at one slaughterhouse with immersion chilling.

scholarly journals Motility as an Intestinal Colonization Factor for Campylobacter jejuni

Microbiology ◽  
1985 ◽  
Vol 131 (8) ◽  
pp. 1973-1980 ◽  
Author(s):  
T. MOROOKA ◽  
A. UMEDA ◽  
K. AMAKO
Keyword(s):  
Campylobacter Jejuni ◽  
Intestinal Colonization ◽  
Colonization Factor

scholarly journals Evaluation of passive immunotherapeutic efficacy of hyperimmunized egg yolk powder against intestinal colonization of Campylobacter jejuni in chickens

2014 ◽  
Vol 93 (11) ◽  
pp. 2779-2787 ◽  
Author(s):  
Narayan C. Paul ◽  
Salma Al-Adwani ◽  
Rocio Crespo ◽  
Devendra H. Shah
Keyword(s):  
Campylobacter Jejuni ◽  
Egg Yolk ◽  
Intestinal Colonization

scholarly journals Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model

mBio ◽  
2013 ◽  
Vol 4 (3) ◽  
Author(s):  
M. John Albert ◽  
Abu Salim Mustafa ◽  
Anjum Islam ◽  
Shilpa Haridas
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Cholera Toxin ◽  
Campylobacter Jejuni ◽  
Adult Mouse ◽  
Outer Membrane Proteins ◽  
Cross Reaction ◽  
Intestinal Colonization ◽  
Content Type ◽  
Cholera Vaccines

ABSTRACTImmunity toCampylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070–3073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of allCampylobacter jejunistrains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization byC. jejuniin an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged withC. jejunistrains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP’s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (P, ≤0.05 to ≤0.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to controlC. jejunidiarrhea with these vaccines.IMPORTANCECampylobacter jejuniis a major cause of diarrhea worldwide. Patients who recover fromC. jejunidiarrhea develop immunity to the infecting serotype and remain susceptible to infection with other serotypes. A vaccine based on a common protective antigen(s) present in allC. jejuniserotypes is expected to provide broad protection. In our previous study, we showed that antibody to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains ofC. jejuni. We assumed that the B subunit of the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, might be the component that cross-reacts with MOMP. In the current study, we showed that orally immunizing mice with CT protected them against colonization upon challenge with different serotypes ofC. jejuni. We also showed that CT-B is the component mediating cross-reaction. Therefore, it might be possible to use cholera vaccines to preventC. jejunidiarrhea. This could result in significant savings in vaccine development and treatment of the disease.

Stability of Related Human and ChickenCampylobacter jejuni Genotypes after Passage through Chick Intestine Studied by Pulsed-Field Gel Electrophoresis

1999 ◽  
Vol 65 (5) ◽  
pp. 2272-2275 ◽  
Author(s):  
Marja-Liisa Hänninen ◽  
Marjaana Hakkinen ◽  
Hilpi Rautelin
Keyword(s):  
Genomic Instability ◽  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Genomic Stability ◽  
Pulsed Field Gel Electrophoresis ◽  
Genomic Rearrangement ◽  
Intestinal Colonization ◽  
Pulsed Field ◽  
Heat Stable ◽  
New Genotype

ABSTRACT The genomic stability of 12 Campylobacter jejunistrains consisting of two groups of human and chicken isolates was studied by analysis of their PFGE (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks’ intestines. The patterns of SmaI, SalI, and SacII digests remained stable after intestinal passage, except for those of two strains. One originally human strain, FB 6371, changed its genotype from II/A (SmaI/SacII) to I/B. Another strain, BTI, originally isolated from a chicken, changed its genotype from I/B to a new genotype. The genomic instability of the strains was further confirmed by SalI digestion and ribotyping of theHaeIII digests. In addition, heat-stable serotype 57 of strain FB 6371 changed to serotype 27 in all isolates with new genotypes but remained unchanged in an isolate with the original genotype. Serotype 27 of strain BTI remained stable. Our study suggests that during intestinal colonization, genomic rearrangement, as demonstrated by changed PFGE and ribopatterns, may occur.

scholarly journals Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

2004 ◽  
Vol 72 (7) ◽  
pp. 3769-3776 ◽  
Author(s):  
Michael A. Jones ◽  
Kerrie L. Marston ◽  
Claire A. Woodall ◽  
Duncan J. Maskell ◽  
Dennis Linton ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Campylobacter Jejuni ◽  
Genome Sequence ◽  
Intestinal Tract ◽  
Intestinal Colonization ◽  
Human Pathogen ◽  
Gut Flora ◽  
High Level

ABSTRACT The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. Experimental colonization and persistence studies were carried out with C. jejuni NCTC11168 by using 2-week-old Light Sussex chickens possessing an acquired natural gut flora. After inoculation, NCTC11168 initially colonized the intestine poorly. However, after 5 weeks we observed adaptation to high-level colonization, which was maintained after in vitro passage. The adapted strain exhibited greatly increased motility. A second strain, C. jejuni 11168H, which had been selected under in vitro conditions for increased motility (A. V. Karlyshev, D. Linton, N. A. Gregson, and B. W. Wren, Microbiology 148:473-480, 2002), also showed high-level intestinal colonization. The levels of colonization were equivalent to those of six other strains, assessed under the same conditions. There were four mutations in C. jejuni 11168H that reduced colonization; maf5, flaA (motility and flagellation), and kpsM (capsule deficiency) eliminated colonization, whereas pglH (general glycosylation system deficient) reduced but did not eliminate colonization. This study showed that there was colonization of the avian intestinal tract by a Campylobacter strain having a known genome sequence, and it provides a model for colonization and persistence studies with specific mutations.

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