Transcriptome Analysis of Indole-3-Butyric Acid-Induced Adventitious Root Formation in Nodal Cuttings of Camellia sinensis (L.)

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

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Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

10.21203/rs.2.16981/v4 ◽

2020 ◽

Author(s):

Haishan An ◽

Jiaying Zhang ◽

Fangjie Xu ◽

Shuang Jiang ◽

Xueying Zhang

Keyword(s):

Candidate Genes ◽

Transcriptome Analysis ◽

Adventitious Root ◽

Root Formation ◽

Transcriptome Profiling ◽

Vaccinium Corymbosum ◽

Adventitious Root Formation ◽

Root Characteristics ◽

Indole Butyric Acid ◽

Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation.Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes (ARFs and SAURs), 13 transcription factors (LOB domain-containing protein (LBDs)), 6 auxin transporters (AUX22, LAX3/5 and PIN-like 6 (PIL6s)) and 6 rooting-associated genes (root meristem growth factor 9 (RGF9), lateral root primordium 1 (LRP1s), and dormancy-associated protein homologue 3 (DRMH3)). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation.Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

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Comprehensive Transcriptome Analysis Unravels the Existence of Crucial Genes Regulating Primary Metabolism during Adventitious Root Formation in Petunia hybrida

PLoS ONE ◽

10.1371/journal.pone.0100997 ◽

2014 ◽

Vol 9 (6) ◽

pp. e100997 ◽

Cited By ~ 25

Author(s):

Amirhossein Ahkami ◽

Uwe Scholz ◽

Burkhard Steuernagel ◽

Marc Strickert ◽

Klaus-Thomas Haensch ◽

...

Keyword(s):

Transcriptome Analysis ◽

Adventitious Root ◽

Petunia Hybrida ◽

Root Formation ◽

Adventitious Root Formation ◽

Primary Metabolism

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Synergistic interaction between coumarin 1,2-benzopyrone and indole-3-butyric acid in stimulating adventitious root formation in Vigna radiata (L.) Wilczek cuttings: I. Endogenous free and conjugated IAA and basic isoperoxidases

Plant Growth Regulation ◽

10.1023/b:grow.0000026507.90898.8a ◽

2004 ◽

Vol 42 (3) ◽

pp. 253-262 ◽

Cited By ~ 13

Author(s):

Kamel Tartoura ◽

Andrea da Rocha ◽

Sahar Youssef

Keyword(s):

Butyric Acid ◽

Vigna Radiata ◽

Adventitious Root ◽

Root Formation ◽

Adventitious Root Formation ◽

Synergistic Interaction

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Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

10.21203/rs.2.16981/v3 ◽

2020 ◽

Author(s):

Haishan An ◽

Jiaying Zhang ◽

Fangjie Xu ◽

Shuang Jiang ◽

Xueying Zhang

Keyword(s):

Candidate Genes ◽

Transcriptome Analysis ◽

Adventitious Root ◽

Root Formation ◽

Transcriptome Profiling ◽

Vaccinium Corymbosum ◽

Adventitious Root Formation ◽

Root Characteristics ◽

Indole Butyric Acid ◽

Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation. Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes ( ARFs and SAURs ), 13 transcription factors ( LOB domain-containing protein ( LBD s)), 6 auxin transporters ( AUX22 , LAX3/5 and PIN-like 6 ( PIL6s )) and 6 rooting-associated genes ( root meristem growth factor 9 ( RGF9 ), lateral root primordium 1 ( LRP1s ), and dormancy-associated protein homologue 3 ( DRMH3 )). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation. Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

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