scholarly journals Study of Mycobacterium tuberculosis Complex Genotypic Diversity in Malaysia Reveals a Predominance of Ancestral East-African-Indian Lineage with a Malaysia-Specific Signature

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e114832 ◽  
Author(s):  
Fazli Ismail ◽  
David Couvin ◽  
Izzah Farakhin ◽  
Zaidah Abdul Rahman ◽  
Nalin Rastogi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Genotypic Diversity ◽  
East African ◽  
Tuberculosis Complex

scholarly journals Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Barbara Tizzano ◽  
Tobias K. Dallenga ◽  
Christian Utpatel ◽  
Jochen Behrends ◽  
Susanne Homolka ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
East African ◽  
Tuberculosis Complex ◽  
Dormant State ◽  
Infection Dynamics ◽  
Central Asian ◽  
Oxygen Starvation ◽  
Similar Survival ◽  
Different Strains

AbstractWhile persistence in a dormant state is crucial for the life cycle of Mycobacterium tuberculosis, no investigation regarding dormancy survival of different strains across different lineages was performed so far. We analyzed responses to oxygen starvation and recovery in terms of growth, metabolism, and transcription. All different strains belonging to the Euro-American lineage (L4) showed similar survival and resuscitation characteristics. Different clinical isolates from the Beijing (L2), East African-Indian (L3), and Delhi/Central Asian (L1) lineage did not survive oxygen starvation. We show that dormancy survival is lineage-dependent. Recovery from O2 starvation was only observed in strains belonging to the Euro-American (L4) lineage but not in strains belonging to different lineages (L1, L2, L3). Thus, resuscitation from dormancy after oxygen starvation is not a general feature of all M. tuberculosis strains as thought before. Our findings are of key importance to understand infection dynamics of non-Euro-American vs Euro-American strains and to develop drugs targeting the dormant state.

scholarly journals Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Lisa Nkatha Micheni ◽  
Kennedy Kassaza ◽  
Hellen Kinyi ◽  
Ibrahim Ntulume ◽  
Joel Bazira
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Genotypic Diversity ◽  
Hiv Incidence ◽  
Nucleotide Polymorphism ◽  
Tuberculosis Complex ◽  
Single Nucleotide ◽  
The World ◽  
Southwestern Uganda

Uganda is among the 22 countries in the world with a high burden of tuberculosis. The southwestern region of the country has consistently registered a high TB/HIV incidence rate. This study is aimed at characterizing the Mycobacterium tuberculosis complex (MTBC) genotypic diversity in southwestern Uganda. A total of 283 sputum samples from patients with pulmonary tuberculosis were genotyped using specific single nucleotide polymorphism markers for lineages 3 and 4. Most of the patients were males with a mean age of 34. The lineage 4 Ugandan family was found to be the most dominant strains accounting for 59.7% of all cases followed by lineage 3 at 15.2%. The lineage 4 non-Ugandan family accounted for 14.5% of all cases while 4.2% showed amplification for both lineage 4 and lineage 3. Eighteen samples (6.4%) of the strains remained unclassified since they could not be matched to any lineage based on the genotyping technique used. This study demonstrates that a wide diversity of strains is causing pulmonary tuberculosis in this region with those belonging to the lineage 4 Ugandan family being more predominant. However, to confirm this, further studies using more discriminative genotyping methods are necessary.

scholarly journals Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1294
Author(s):  
Cristina Blanco Blanco Vázquez ◽  
Thiago Doria Barral ◽  
Beatriz Romero ◽  
Manuel Queipo ◽  
Isabel Merediz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Road Traffic ◽  
Mycobacterium Tuberculosis Complex ◽  
Temporal Distribution ◽  
Meles Meles ◽  
Interspecies Transmission ◽  
Tuberculosis Complex ◽  
Serum Samples ◽  
Study Objective ◽  
Infection Pressure

The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.

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