scholarly journals Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120171 ◽  
Author(s):  
Yi-Cheng Chen ◽  
Pi-Ju Tsai ◽  
Yaw-Bin Huang ◽  
Pao-Chu Wu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Simultaneous Determination ◽  
Benzoyl Peroxide ◽  
High Performance ◽  
Chromatographic Condition

Dispersive liquid–liquid microextraction for preconcentration and determination of phenytoin in real samples using response surface methodology-high performance liquid chromatography

RSC Advances ◽  
2014 ◽  
Vol 4 (107) ◽  
pp. 62190-62196 ◽  
Author(s):  
Eslam Pourbasheer ◽  
Samira Sadafi ◽  
Mohammad Reza Ganjali ◽  
Maryam Abbasghorbani
Keyword(s):  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Response Surface ◽  
High Performance ◽  
Real Samples ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

In the present study, dispersive liquid–liquid microextraction (DLLME) was developed for preconcentration and determination of phenytoin in real samples by high performance liquid chromatography (HPLC).

Multivariate Optimization of Separation Conditions for Simultaneous Determination of Acemetacin and Chlorzoxazone in a Pharmaceutical Preparation by HPLC Using Response Surface Methodology

2013 ◽  
Vol 96 (4) ◽  
pp. 723-729
Author(s):  
Cigdem Aybaba ◽  
Ismail Murat Palabiyik ◽  
Mehmet Gokhan Caglayan ◽  
Feyyaz Onur
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Pharmaceutical Preparation ◽  
Internal Standard ◽  
Hplc Method ◽  
Optimum Separation

Abstract A new, fast, accurate, precise, and sensitive RP-HPLC method for the simultaneous determination of acemetacin and chlorzoxazone has been developed. Response surface methodology with a central composite design was used to optimize the acetonitrile and ammonium acetate percentage in the mobile phase and pH of ammonium acetate. The optimum separation was achieved on a C18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase methanol–acetonitrile–0.02 M ammonium acetate, pH 9.4 (25 + 35 + 40, v/v/v) at a flow rate of 1.5 mL/min; UV detection at 270 nm, and cyanocobalamin as an internal standard. This developed method was validated and successfully applied to a coated tablet pharmaceutical preparation.

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