Removal of Transmissible Spongiform Encephalopathy Prion from Large Volumes of Cell Culture Media Supplemented with Fetal Bovine Serum by Using Hollow Fiber Anion-Exchange Membrane Chromatography

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation

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Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Toxicology in Vitro ◽

10.1016/j.tiv.2010.03.016 ◽

2010 ◽

Vol 24 (4) ◽

pp. 1053-1063 ◽

Cited By ~ 281

Author(s):

J. van der Valk ◽

D. Brunner ◽

K. De Smet ◽

Å. Fex Svenningsen ◽

P. Honegger ◽

...

Keyword(s):

Cell Culture ◽

Bovine Serum ◽

Culture Media ◽

Fetal Bovine Serum ◽

Cell Culture Media ◽

In Vitro Methods ◽

Fetal Bovine

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Fetal bovine serum—a cell culture dilemma

Science ◽

10.1126/science.abm1317 ◽

2022 ◽

Vol 375 (6577) ◽

pp. 143-144

Author(s):

Jan van der Valk

Keyword(s):

Cell Culture ◽

Bovine Serum ◽

Culture Media ◽

Fetal Bovine Serum ◽

Cell Culture Media ◽

A Cell ◽

Fetal Bovine

Ethical and possible reproducibility issues arise when using fetal bovine serum in cell culture media

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Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013001300004 ◽

2013 ◽

Vol 33 (suppl 1) ◽

pp. 20-24 ◽

Cited By ~ 1

Author(s):

Gesiane Ribeiro ◽

Cristina O. Massoco ◽

José Corrêa de Lacerda Neto

Keyword(s):

Cell Culture ◽

Bone Marrow ◽

Adipose Tissue ◽

Culture Media ◽

Stromal Vascular Fraction ◽

Bone Marrow Aspiration ◽

Cell Culture Media ◽

Colony Forming Units ◽

Fetal Bovine ◽

Equine Bone

The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.

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The Influence of Micronutrients in Cell Culture: A Reflection on Viability and Genomic Stability

BioMed Research International ◽

10.1155/2013/597282 ◽

2013 ◽

Vol 2013 ◽

pp. 1-22 ◽

Cited By ~ 36

Author(s):

Ana Lúcia Vargas Arigony ◽

Iuri Marques de Oliveira ◽

Miriana Machado ◽

Diana Lilian Bordin ◽

Lothar Bergter ◽

...

Keyword(s):

Cell Culture ◽

Cultured Cells ◽

Culture Media ◽

Genomic Stability ◽

Sole Source ◽

Cell Culture Media ◽

The Media ◽

Fetal Bovine

Micronutrients, including minerals and vitamins, are indispensable to DNA metabolic pathways and thus are as important for life as macronutrients. Without the proper nutrients, genomic instability compromises homeostasis, leading to chronic diseases and certain types of cancer. Cell-culture media try to mimic thein vivoenvironment, providingin vitromodels used to infer cells' responses to different stimuli. This review summarizes and discusses studies of cell-culture supplementation with micronutrients that can increase cell viability and genomic stability, with a particular focus on previousin vitroexperiments. In these studies, the cell-culture media include certain vitamins and minerals at concentrations not equal to the physiological levels. In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5–10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells. Further studies better evaluating micronutrients' roles at a molecular level and influence on the genomic stability of cells are still needed.

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