Fetal bovine serum—a cell culture dilemma

Science ◽  
2022 ◽  
Vol 375 (6577) ◽  
pp. 143-144
Author(s):  
Jan van der Valk
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
A Cell ◽  
Fetal Bovine

Ethical and possible reproducibility issues arise when using fetal bovine serum in cell culture media

scholarly journals A plea to reduce or replace fetal bovine serum in cell culture media

2013 ◽  
Vol 65 (5) ◽  
pp. 791-793 ◽  
Author(s):  
Gerhard Gstraunthaler ◽  
Toni Lindl ◽  
Jan van der Valk
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
Fetal Bovine

Preferential adsorption of fetal bovine serum on bare and aromatic thiol-functionalized gold surfaces in cell culture media

2011 ◽  
Vol 363 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Jin Park ◽  
Jin-Ho Park ◽  
Kwang-Su Ock ◽  
Erdene-Ochir Ganbold ◽  
Nam Woong Song ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Gold Surfaces ◽  
Cell Culture Media ◽  
Aromatic Thiol ◽  
Preferential Adsorption ◽  
Fetal Bovine

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

2010 ◽  
Vol 24 (4) ◽  
pp. 1053-1063 ◽  
Author(s):  
J. van der Valk ◽  
D. Brunner ◽  
K. De Smet ◽  
Å. Fex Svenningsen ◽  
P. Honegger ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cell Culture Media ◽  
In Vitro Methods ◽  
Fetal Bovine

scholarly journals Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions

2019 ◽  
Vol 10 (1) ◽  
pp. 7
Author(s):  
Adi Santoso ◽  
Larasati Larasati ◽  
Arizah Kusumawati ◽  
Popi Hadi Wisnuwardhani ◽  
Ratih Asma Ningrum ◽  
...  
Keyword(s):  
Cell Culture ◽  
Protein Expression ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Culture Conditions ◽  
Expression Level ◽  
Seeding Density ◽  
Cell Culture Media ◽  
Human Erythropoietin ◽  
Fetal Bovine

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation

scholarly journals Fetal Bovine Serum-Derived Extracellular Vesicles Persist within Vesicle-Depleted Culture Media

2018 ◽  
Vol 19 (11) ◽  
pp. 3538 ◽  
Author(s):  
Brandon Lehrich ◽  
Yaxuan Liang ◽  
Pooya Khosravi ◽  
Howard Federoff ◽  
Massimo Fiandaca
Keyword(s):  
Cell Growth ◽  
Extracellular Vesicles ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Cellular Growth ◽  
Primary Astrocyte ◽  
Fetal Bovine ◽  
Cell Growth And Viability

It is known that culture media (CM) promotes cellular growth, adhesion, and protects explanted primary brain cells from in vitro stresses. The fetal bovine serum (FBS) supplement used in most CM, however, contains significant quantities of extracellular vesicles (EVs) that confound quantitative and qualitative analyses from the EVs produced by the cultured cells. We quantitatively tested the ability of common FBS EV-depletion protocols to remove exogenous EVs from FBS-supplemented CM and evaluated the influence such methods have on primary astrocyte culture growth and viability. We assessed two methodologies utilized for FBS EV removal prior to adding to CM: (1) an 18-h ultracentrifugation (UC); and (2) a commercial EV-depleted FBS (Exo-FBS™). Our analysis demonstrated that Exo-FBS™ CM provided the largest depletion (75%) of total FBS EVs, while still providing 6.92 × 109 ± 1.39 × 108 EVs/mL. In addition, both UC and Exo-FBS™ CM resulted in poor primary astrocyte cell growth and viability in culture. The two common FBS EV-depletion methods investigated, therefore, not only contaminate in vitro primary cell-derived EV analyses, but also provide a suboptimal environment for primary astrocyte cell growth and viability. It appears likely that future CM optimization, using a serum-free alternative, might be required to advance analyses of cell-specific EVs isolated in vitro.

Fetal bovine serum induces changes in fatty acid composition ofTrypanosoma cruziphosphoinositides

1995 ◽  
Vol 41 (10) ◽  
pp. 951-954 ◽  
Author(s):  
G. Racagni ◽  
M. G. de Lema ◽  
G. Hernández ◽  
E. E. Machado-Domenech
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Trypanosoma Cruzi ◽  
Acid Composition ◽  
Bovine Serum ◽  
Culture Medium ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have described the fatty acid composition of the total lipids and of the phosphoinositide fractions. Additionally, we have reported the increase of polyphosphoinositides and phosphatidic acid after cholinergic stimulation. Since further attempts to reproduce these results with 5% FBS in the culture medium were not successful, the effect of the FBS concentration on the fatty acid composition of phospholipids from the T. cruzi epimastigote forms was thoroughly examined. This work showed that when the FBS concentration supplementing the culture medium was reduced from 10 to 5%, the fatty acid composition of the phosphoinositides was altered while the other major phospholipids were not significantly affected. The most relevant result was the decrease in the content of linoleic acid (18:2) and the increase of palmitoleic acid (16:1) in phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol (PI) and phosphatidylinositol phosphate also exhibited similar changes in the same fatty acids. The C2fatty acid composition of the phosphoinositides, under the same conditions, is also reported here for the first time.Key words: Trypanosoma cruzi, fatty acids, phosphoinositides, fetal bovine serum, phospholipids.

241 9.22 Performance of Gamma-irradiated Fetal Bovine Serum in Cell Culture

1994 ◽  
pp. 99-101
Author(s):  
J.D. Keathley ◽  
D. Wyatt ◽  
C.M. Williams ◽  
R. Festen ◽  
C. Maben
Keyword(s):  
Cell Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Gamma Irradiated
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