Genetic diversity and association mapping in the Colombian Central Collection of Solanum tuberosum L. Andigenum group using SNPs markers

Potato (Solanum tuberosum L.) is a versatile crop and a source of inexpensive energy in the human diet in many countries. It can be used as fresh products and commercially processed foods such as French fries and chips. Potato varieties development research previously conducted in Ethiopia related to processing quality were limited in their scope of quality parameters. This experiment was conducted at Holetta Agricultural Research Centre, Ethiopia during the main crop season of 2017. Twenty-four potato genotypes were evaluated for 23 quantitative and six qualitative traits in randomized complete block design with three replications to determining the nature and magnitude of common genetic diversity and to screen out genetically diverse parents by using cluster and principal component analysis. The first eight principal components accounted for 90.26% of the observed variations among 24 potato genotypes. The first three PC accounted for 60.43% of the variation. The genetic distances among the 24 potato genotypes ranged from 3.40 to 11.80 and the genotypes were grouped into eight clusters based on quantitative and qualitative traits. Cluster II consisted of 25%, Cluster IV, I, III contained 20.83%, 16.67% and 12.5% of genotypes, respectively, while Cluster VI, VII and VIII each consisted of one genotype. In conclusion, genotypes grouped under Cluster II and VIII worth further evaluation to obtain genotypes with highest total tuber yield, the specific gravity of tuber, dry matter content, total starch content, acceptable tuber physical and frying quality with other desirable traits.

Download Full-text

Keragaman Genetik Beberapa Varietas Kentang (Solanum tuberosum L.) Berdasarkan Penanda Random Amplified Polimorphic DNA (RAPD) (Genetic Diversity of Several Varieties of Potato (Solanumtuberosum L) Based on Random Amplified Polymorphic DNA (RAPD))

JURNAL BIOS LOGOS ◽

10.35799/jbl.6.2.2016.13794 ◽

2016 ◽

Vol 6 (2) ◽

Author(s):

Yefta B Kawengian ◽

Edy Lengkong ◽

Jeany Mandang

Keyword(s):

Genetic Diversity ◽

Solanum Tuberosum ◽

Genetic Information ◽

Solanum Tuberosum L ◽

Random Amplified Polymorphic Dna ◽

Rapd Marker ◽

Potato Production ◽

Coding Region ◽

Protein Coding ◽

Coding Regions

Abstrak Pengembangan tanaman kentang (Solanum tuberosum L.) unggul untuk menunjang kebutuhan produksi kentang yang terus meningkat membutuhkan tersedianya informasi genetik tanaman kentang yang ada. Informasi keragaman genetik dapat diperoleh menggunakan penanda molekuler RAPD yang dapat mendeteksi keragaman sampai pada tingkat DNA, baik pada daerah penyandi atau bukan penyandi protein dengan cara mendeteksi polimorfik sekuens nukleotida. Informasi yang diperoleh akurat karena tidak dipengaruhi lingkungan. Penelitian ini bertujuan untuk menganalisis keragaman genetik dari kentang kultivar Superjhon, Atlantik, Dessire, Nadia dan Granola menggunakan penanda RAPD. Hasil penelitian menunjukkan bahwa dari 14 primer acak yang digunakan hanya 7 yang memberikan pola pita DNA yang polimorfik dan 16 pita dari total 28 pita DNA yang dihasilkan (57 %) merupakan pita DNA polimorfik. Rata-rata keragaman genetik kentang sebesar 26,8 %. Keragaman genetik terkecil (15,4 %) adalah antara kentang Atlantic dan Superjhon, sedangkan keragaman terbesar (57,7 %) antara kentang Nadia dan Dessire. Hasil analisis pengelompokan menunjukkan tanaman mengelompok berdasarkan sifat/karakter dan asalnya. Kata kunci: kentang, keragaman genetik, RAPD Abstract The information of potato genetic diversity are required to support the increasing potato demands in the superior potato production. The information of genetic diversity can be obtained using RAPD molecular marker. RAPD can detect the genetic diversity at the DNA level, both in the coding region and non-protein-coding regions by detecting polymorphic sequences in nucleotides. This method provide accurate genetic information because it is not influenced by the environment. This study was conducted to analyze the genetic diversity of potato Superjhon, Atlantic, Dessire, Nadia and Granola using RAPD marker. Amongst the 14 random primers, only 7 primers produced polymorphic banding pattern. Sixteen DNA bands of total 28 existed DNA bands (57%) were polymorphic. The average of genetic diversity was 26.8 %. The smallest genetic diversity (15.4%) was between Atlantic and Superjhon, whereas the greatest genetic diversity (57.7%) was between Nadia and Dessire. The analysis results showed that potato clustered grouping was based on their characters and their origins. Keywords: genetic diversity, potatoes, RAPD

Download Full-text

GENERATING GENETIC DIVERSITY THROUGH DIALLEL CROSSES OF PROMISING POTATO CULTIVARS (Solanum tuberosum L.) AND STUDYING CULTIVAR HYBRIDS UNDER WATER DEFICIT STRESS

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i2.4511 ◽

2020 ◽

Vol 7 (2) ◽

pp. 49-56

Author(s):

Mohammad Reza Vesali ◽

Reza Baradaran ◽

Davoud Hassanpanah ◽

Mohammad Javad Seghatoleslami

Keyword(s):

Genetic Diversity ◽

Solanum Tuberosum ◽

Water Deficit ◽

Solanum Tuberosum L ◽

Potato Cultivars ◽

Water Deficit Stress ◽

Diallel Crosses

Download Full-text

A Review on Potato (Solanum Tuberosum L.) and its Genetic Diversity

International Journal of Genetics ◽

10.9735/0975-2862.10.2.360-364 ◽

2018 ◽

Vol 10 (2) ◽

pp. 360 ◽

Cited By ~ 1

Author(s):

B. J. Reddy ◽

R. Mandal ◽

M. Chakroborty ◽

L. Hijam ◽

P. Dutta

Keyword(s):

Genetic Diversity ◽

Solanum Tuberosum ◽

Solanum Tuberosum L

Download Full-text

Evaluation of genetic diversity for yield and quality parameters of different potato (Solanum tuberosum L.) germplasm

Journal of Applied and Natural Science ◽

10.31018/jans.v7i1.596 ◽

2015 ◽

Vol 7 (1) ◽

pp. 235-241

Author(s):

Sanjay Datta ◽

Rajib Das ◽

Dhirendra Singh

Keyword(s):

Genetic Diversity ◽

Solanum Tuberosum ◽

Genetic Divergence ◽

Solanum Tuberosum L ◽

Research Centre ◽

Dry Matter Content ◽

Quality Traits ◽

Yield And Quality ◽

Cluster Distance ◽

Growth Characters

An investigation was carried out at Vegetable Research Centre, G.B. Pant University of Agriculture and Technology, Pantnagar during spring-summer season 2011 and 2012 to study the genetic diversity using Mahalanobis’s D2 – technique among thirty five potato (Solanum tuberosum L.) germplasm for important yield attributing and quality traits. The D2 values were calculated and thirty five potato genotypes were grouped into nine clusters for growth characters and ten clusters for quality traits respectively. All the genotypes included in the present investigation, were indigenous, but their grouping in different clusters, suggested that genotypes did not follow the geographic distribution. The cluster I contained the maximum number of genotypes with respect to both yield attributing and quality traits. The inter cluster distance in most of the cases were higher than the intra-cluster distance indicating wider genetic diversity among the genotypes of different groups. Average tuber weight of potato plant contributed maximum (31.76%), followed by number of tuber per plant (27.56%), internodal length (14.45%) and plant dry matter content (13.61%) for growth characters. For quality characters, ascorbic acid content (24.70%), protein content of tuber (20.84%) and TSS of tuber (20.00%) contributed effectively towards genetic divergence. So, these traits will offer a good scope for improvement of yield and quality through rational selection of parental genotypes for future potato breeding. The findings indicated that use of parents selected from the same cross or from a cross involving a common parent should be avoided in hybridization. The results broadly showed there was no parallelism between geographical and genetic divergence.

Download Full-text