scholarly journals Crystal structures of Moorella thermoacetica cyanuric acid hydrolase reveal conformational flexibility and asymmetry important for catalysis

PLoS ONE ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. e0216979 ◽  
Author(s):  
Ke Shi ◽  
Seunghee Cho ◽  
Kelly G. Aukema ◽  
Thomas Lee ◽  
Asim K. Bera ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Cyanuric Acid ◽  
Conformational Flexibility ◽  
Acid Hydrolase ◽  
Moorella Thermoacetica

scholarly journals Thermostable Cyanuric Acid Hydrolase from Moorella thermoacetica ATCC 39073

2009 ◽  
Vol 75 (22) ◽  
pp. 6986-6991 ◽  
Author(s):  
Qingyan Li ◽  
Jennifer L. Seffernick ◽  
Michael J. Sadowsky ◽  
Lawrence P. Wackett
Keyword(s):  
Low Temperatures ◽  
Cyanuric Acid ◽  
Water Remediation ◽  
Acid Hydrolase ◽  
Swimming Pools ◽  
Acid Hydrolases ◽  
Metabolic Intermediate ◽  
Moorella Thermoacetica ◽  
Trichloroisocyanuric Acid ◽  
Structurally Stable

ABSTRACT Cyanuric acid, a metabolic intermediate in the degradation of many s-triazine compounds, is further metabolized by cyanuric acid hydrolase. Cyanuric acid also accumulates in swimming pools due to the breakdown of the sanitizing agents di- and trichloroisocyanuric acid. Structurally stable cyanuric acid hydrolases are being considered for usage in pool water remediation. In this study, cyanuric acid hydrolase from the thermophile Moorella thermoacetica ATCC 39073 was cloned, expressed in Escherichia coli, and purified to homogeneity. The recombinant enzyme was found to have a broader temperature range and greater stability, at both elevated and low temperatures, than previously described cyanuric acid hydrolases. The enzyme had a narrow substrate specificity, acting only on cyanuric acid and N-methylisocyanuric acid. The M. thermoacetica enzyme did not require metals or other discernible cofactors for activity. Cyanuric acid hydrolase from M. thermoacetica is the most promising enzyme to use for cyanuric acid remediation applications.

scholarly journals Plasmid Localization and Organization of Melamine Degradation Genes in Rhodococcus sp. Strain Mel

2011 ◽  
Vol 78 (5) ◽  
pp. 1397-1403 ◽  
Author(s):  
Anthony G. Dodge ◽  
Lawrence P. Wackett ◽  
Michael J. Sadowsky
Keyword(s):  
454 Pyrosequencing ◽  
Minimal Medium ◽  
Doubling Time ◽  
Cyanuric Acid ◽  
Acid Hydrolase ◽  
Content Type ◽  
N Source ◽  
Genes Encoding ◽  
Rhodococcus Sp ◽  
Roche 454

ABSTRACTRhodococcussp. strain Mel was isolated from soil by enrichment and grew in minimal medium with melamine as the sole N source with a doubling time of 3.5 h. Stoichiometry studies showed that all six nitrogen atoms of melamine were assimilated. The genome was sequenced by Roche 454 pyrosequencing to 13× coverage, and a 22.3-kb DNA region was found to contain a homolog to the melamine deaminase genetrzA. Mutagenesis studies showed that the cyanuric acid hydrolase and biuret hydrolase genes were clustered together on a different 17.9-kb contig. Curing and gene transfer studies indicated that 4 of 6 genes required for the complete degradation of melamine were located on an ∼265-kb self-transmissible linear plasmid (pMel2), but this plasmid was not required for ammeline deamination. TheRhodococcussp. strain Mel melamine metabolic pathway genes were located in at least three noncontiguous regions of the genome, and the plasmid-borne genes encoding enzymes for melamine metabolism were likely recently acquired.

X-ray studies on crystalline complexes involving amino acids and peptides. XL. Conformational variability, recurring and new features of aggregation, and effect of chirality in the malonic acid complexes of DL- and L-arginine

2002 ◽  
Vol 58 (6) ◽  
pp. 1051-1056 ◽  
Author(s):  
N. T. Saraswathi ◽  
M. Vijayan
Keyword(s):  
Amino Acids ◽  
Hydrogen Bonding ◽  
Amino Acid ◽  
Crystal Structures ◽  
Malonic Acid ◽  
Conformational Flexibility ◽  
X Ray ◽  
Conformational Variability ◽  
Aggregation Pattern ◽  
Positively Charged

The crystal structures of the complexes of malonic acid with DL- and L-arginine, which contain positively charged argininium ions and negatively charged semimalonate ions, further demonstrate the conformational flexibility of amino acids. A larger proportion of folded conformations than would be expected on the basis of steric consideration appears to occur in arginine, presumably because of the requirements of hydrogen bonding. The aggregation pattern in the DL-arginine complex bears varying degrees of resemblance to patterns observed in other similar structures. An antiparallel hydrogen-bonded dimeric arrangement of arginine, and to a lesser extent lysine, is a recurring motif. Similarities also exist among the structures in the interactions with this motif and its assembly into larger features of aggregation. However, the aggregation pattern observed in the L-arginine complex differs from any observed so far, which demonstrates that all the general patterns of amino-acid aggregation have not yet been elucidated. The two complexes represent cases where the reversal of the chirality of half the amino-acid molecules leads to a fundamentally different aggregation pattern.

scholarly journals Structure of the Cyanuric Acid Hydrolase TrzD Reveals Product Exit Channel

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Asim K Bera ◽  
Kelly G. Aukema ◽  
Mikael Elias ◽  
Lawrence P. Wackett
Keyword(s):  
Cyanuric Acid ◽  
Exit Channel ◽  
Acid Hydrolase

scholarly journals Defining Sequence Space and Reaction Products within the Cyanuric Acid Hydrolase (AtzD)/Barbiturase Protein Family

2012 ◽  
Vol 194 (17) ◽  
pp. 4579-4588 ◽  
Author(s):  
J. L. Seffernick ◽  
J. S. Erickson ◽  
S. M. Cameron ◽  
S. Cho ◽  
A. G. Dodge ◽  
...  
Keyword(s):  
Sequence Space ◽  
Cyanuric Acid ◽  
Protein Family ◽  
Acid Hydrolase ◽  
Reaction Products ◽  
Defining Sequence

The Preparation and Crystal Structures of a Series of Urea Adducts: with Fumaric Acid (2 : 1), with Itaconic Acid (1 : 1) and with Cyanuric Acid (1 : 1)

1997 ◽  
Vol 50 (10) ◽  
pp. 1021 ◽  
Author(s):  
Graham Smith ◽  
Colin H. L. Kennard ◽  
Karl A. Byriel
Keyword(s):  
Hydrogen Bonding ◽  
Crystal Structures ◽  
Itaconic Acid ◽  
Fumaric Acid ◽  
Cyanuric Acid ◽  
X Ray ◽  
X Ray Crystallography ◽  
Donor And Acceptor ◽  
Bonding Systems ◽  
Urea Adducts

The adducts of a series of compounds with urea (ur) have been prepared and their crystal structures determined by X-ray crystallography. These are the adducts with the unsaturated aliphatic acids fumaric acid [(fumaric acid)(ur)2] (1) and itaconic acid [(itaconic acid)(ur)] (2), and with cyanuric acid [(cyanuric acid)(ur)] (3). All structures have extensive hydrogen-bonding systems in which most of the urea donor and acceptor sites are involved.

scholarly journals Crystal structures of ten enantiopure Schiff bases bearing a naphthyl group

2016 ◽  
Vol 72 (4) ◽  
pp. 583-589 ◽  
Author(s):  
Guadalupe Hernández-Téllez ◽  
Gloria E. Moreno ◽  
Sylvain Bernès ◽  
Angel Mendoza ◽  
Oscar Portillo ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Schiff Bases ◽  
Conformational Flexibility ◽  
Solvent Free ◽  
Intermolecular Contacts ◽  
Methyl Naphthalene

Using a general solvent-free procedure for the synthesis of chiral Schiff bases, the following compounds were synthesized and their crystal structures determined: (S)-(+)-2-{[(1-phenylethyl)imino]methyl}naphthalene, C19H17N, (1), (S)-(+)-2-({[(4-methylphenyl)ethyl]imino}methyl)naphthalene, C20H19N, (2), (R)-(−)-2-({[(4-methoxylphenyl)ethyl]imino}methyl)naphthalene, C20H19NO, (3), (R)-(−)-2-({[(4-fluorophenyl)ethyl]imino}methyl)naphthalene, C19H16FN, (4), (S)-(+)-2-({[(4-chlorophenyl)ethyl]imino}methyl)naphthalene, C19H16ClN, (5), (S)-(+)-2-({[(4-bromophenyl)ethyl]imino}methyl)naphthalene, C19H16BrN, (6), (S)-(+)-2-({[1-(naphthalen-1-yl)ethyl]imino}methyl)naphthalene, C23H19N, (7), (S)-(+)-2-{[(1-cyclohexylethyl)imino]methyl}naphthalene, C19H23N, (8), (S)-(−)-2-{[(1,2,3,4-tetrahydronaphthalen-1-yl)imino]methyl}naphthalene, C21H19N, (9), and (+)-2-({[(1S,2S,3S,5R)-2,6,6-trimethylbicyclo[3.1.1]hept-3-yl]imino}methyl}naphthalene, C21H25N, (10). The moiety provided by the amine generates conformational flexibility for these imines. In the crystals, no strong intermolecular contacts are observed, in spite of the presence of aromatic groups.

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