scholarly journals Retraction: Perthamide C Inhibits eNOS and iNOS Expression and Has Immunomodulating Activity In Vivo

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0222175
Author(s):  
Keyword(s):  
Inos Expression ◽  
Immunomodulating Activity

scholarly journals Perthamide C Inhibits eNOS and iNOS Expression and Has Immunomodulating Activity In Vivo

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57801 ◽  
Author(s):  
Mariarosaria Bucci ◽  
Anna Cantalupo ◽  
Valentina Vellecco ◽  
Elisabetta Panza ◽  
Maria Chiara Monti ◽  
...  
Keyword(s):  
Inos Expression ◽  
Immunomodulating Activity

In vivo immunomodulating activity of wood extracts from Clausena excavata Burm. f.

2005 ◽  
Vol 102 (1) ◽  
pp. 5-9 ◽  
Author(s):  
A. Manosroi ◽  
A. Saraphanchotiwitthaya ◽  
J. Manosroi
Keyword(s):  
Immunomodulating Activity ◽  
Wood Extracts

Abstract 28: Increased Expression Of Mitochondrial Inducible Nitric Oxide Synthase By Heat Shock Protein Hsp22/h11 Kinase Promotes Oxidative Phosphorylation In Mammalian Heart

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Hongyu Qiu ◽  
Eman Rashed ◽  
Christophe Depre
Keyword(s):  
Nitric Oxide ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Inos Expression ◽  
Mitochondrial Localization ◽  
Over Expression ◽  
Mitochondrial Translocation ◽  
Oxide Synthase ◽  
Stimulation Of

Aims: Stress-inducible heat shock protein 22 (Hsp22) confers protection against ischemia through induction of the inducible isoform of nitric oxide synthase (iNOS). Hsp22 over-expression in vivo significantly stimulates cardiac mitochondrial respiration, whereas Hsp22 deletion in vivo shows a reciprocal effect. It has also been shown in Drosophila that Hsp22 is expressed in the mitochondria that depends on its N-terminal domain. We hypothesized that Hsp22-mediated regulation of mitochondrial function is dependent upon its mitochondrial translocation together with iNOS. Methods and Results: Adenoviruses harboring either the full coding sequence of Hsp22 (Ad-WT-Hsp22) or a mutant lacking a 20 amino acid putative N-terminal mitochondrial localization sequence (Ad-N20-Hsp22) were generated, and infected in rat neonatal cardiomyocytes. Compared to β-Gal control, Ad-WT-Hsp22 accumulated in mitochondria by 2.5 fold (P<0.05), reduced chelerythrine-induced apoptosis by 60% (P<0.01), and increased oxygen consumption rate by 2-fold (P<0.01). This latter effect was abolished upon addition of the specific iNOS inhibitor, 1400W. Ad-WT-Hsp22 significantly increased global iNOS expression by about 2-fold (P<0.01), and also increased its mitochondrial localization by 2.5 fold vs β-gal (P<0.05). Upon comparable over-expression, the Ad-N20-Hsp22 mutant did not show significant mitochondrial translocation, protection against apoptosis or stimulation of mitochondrial respiration. Although Ad-N20-Hsp22 did increase global iNOS expression by 6-fold it did not significantly promote iNOS mitochondrial translocation. Conclusion: Translocation of both Hsp22 and iNOS to the mitochondria is necessary for the stimulation of oxidative metabolism and protection against apoptosis.

Systemic NO production during (septic) shock depends on parenchymal and not on hematopoietic cells: in vivo iNOS expression pattern in (septic) shock

2006 ◽  
Vol 20 (13) ◽  
pp. 2363-2365 ◽  
Author(s):  
Jennyfer Bultinck ◽  
Patrick Sips ◽  
Luc Vakaet ◽  
Peter Brouckaert ◽  
Anje Cauwels ◽  
...  
Keyword(s):  
Septic Shock ◽  
Expression Pattern ◽  
Hematopoietic Cells ◽  
Inos Expression ◽  
No Production

scholarly journals A Polysaccharide Isolated from Codonopsis pilosula with Immunomodulation Effects Both In Vitro and In Vivo

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3632 ◽  
Author(s):  
Yuan-Feng Zou ◽  
Yan-Yun Zhang ◽  
Yu-Ping Fu ◽  
Kari Inngjerdingen ◽  
Berit Paulsen ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Immunoglobulin A ◽  
Transforming Growth Factor Β ◽  
Type I ◽  
Immunomodulating Activity ◽  
Acidic Polysaccharide ◽  
Codonopsis Pilosula ◽  
Intestinal Immune System

In this study, an acidic polysaccharide from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (WCP-I) and its main fragment, WCP-Ia, obtained after pectinase digestion, were structurally elucidated and found to consist of a rhamnogalacturonan I (RG-I) region containing both arabinogalactan type I (AG-I) and type II (AG-II) as sidechains. They both expressed immunomodulating activity against Peyer’s patch cells. Endo-1,4-β-galactanase degradation gave a decrease of interleukine 6 (IL-6) production compared with native WCP-I and WCP-Ia, but exo-α-l-arabinofuranosidase digestion showed no changes in activity. This demonstrated that the stimulation activity partly disappeared with removal of β-d-(1→4)-galactan chains, proving that the AG-I side chain plays an important role in immunoregulation activity. WCP-Ia had a better promotion effect than WCP-I in vivo, shown through an increased spleen index, higher concentrations of IL-6, transforming growth factor-β (TGF-β), and tumor necrosis factor-α (TNF-α) in serum, and a slight increment in the secretory immunoglobulin A (sIgA) and CD4+/CD8+ T lymphocyte ratio. These results suggest that β-d-(1→4)-galactan-containing chains in WCP-I play an essential role in the expression of immunomodulating activity. Combining all the results in this and previous studies, the intestinal immune system might be the target site of WCP-Ia.

Specific iNOS-targeted antisense knockdown in endothelial cells

2003 ◽  
Vol 285 (2) ◽  
pp. C489-C498 ◽  
Author(s):  
Karsten Hemmrich ◽  
Christoph V. Suschek ◽  
Guido Lerzynski ◽  
Oliver Schnorr ◽  
Victoria Kolb-Bachofen
Keyword(s):  
Endothelial Cells ◽  
Antisense Oligonucleotides ◽  
Inos Expression ◽  
Inos Mrna ◽  
Inflammatory Conditions ◽  
Antisense Knockdown ◽  
Oxide Synthase ◽  
The Impact ◽  
Inducible Nitric Oxide

The inhibition of inducible nitric oxide synthase (iNOS) expression via antisense oligonucleotides (AS-ODN) may represent a highly specific tool. Endothelial cells (EC) represent prime candidate cells for in vivo application, and we therefore aimed at optimizing this technique for effectiveness and specificity in primary nontransformed rat EC. EC or L929 fibroblasts were incubated with iNOS-specific ODN optimizing all experimental steps. We find that ODN uptake, as analyzed by fluorescence microscopy and labeled ODN, was absolutely dependent on vehicle presence, and among the vehicles tested, Lipofectin displayed negligible toxicity and good uptake. In addition, omission of serum was also essential, a factor that might limit its use in vivo. Moreover, intranuclear accumulation of AS-ODN appeared crucial for successive inhibition. The impact of ODN on iNOS mRNA, protein, and enzyme activity was specific and resulted in >95% inhibition of protein formation. In conclusion, in this article we provide a protocol for an optimized AS-mediated knockdown, representing a specific and efficient instrument for blocking of iNOS formation and allowing for studying the impact of iNOS expression on endothelial function. We also expose application problems of this technique when working in inflammatory conditions.

scholarly journals Terlipressin inhibits in vivo aortic iNOS expression induced by lipopolysaccharide in rats with biliary cirrhosis

Hepatology ◽  
2002 ◽  
Vol 36 (5) ◽  
pp. 1070-1078 ◽  
Author(s):  
Richard Moreau ◽  
Eric Barrière ◽  
Khalid A. Tazi ◽  
Bernard Lardeux ◽  
Delphine Dargère ◽  
...  
Keyword(s):  
Inos Expression ◽  
Biliary Cirrhosis

scholarly journals Immunomodulatory Activity of a Traditional Sri Lankan Concoction of Coriandrum sativum L. and Coscinium fenestratum G.

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Shashika Dinethri Kothalawala ◽  
Daniya Edward ◽  
Jayamini C. Harasgama ◽  
Loshini Ranaweera ◽  
Ovitigala Vithanage Don Sisira Jagathpriya Weerasena ◽  
...  
Keyword(s):  
Significant Inhibition ◽  
Inos Expression ◽  
Inflammatory Activity ◽  
Immunomodulatory Activity ◽  
No Production ◽  
Sri Lankan ◽  
Paw Edema ◽  
Peritoneal Cells ◽  
Anti Inflammatory

Objective. To investigate the immunomodulatory activity of a traditional Sri Lankan concoction of Coriandrum sativum L. and Coscinium fenestratum (Gaertn.) Colebr., which is a Sri Lankan traditional medicine used to relieve inflammation and cold. Methods. In vivo anti-inflammatory activity was tested using carrageenan-induced rat paw-edema model. Mechanism of anti-inflammatory activity was assessed by investigating the production of nitric oxide (NO), expression of iNOS enzyme, and reactive oxygen species (ROS) by rat peritoneal cells. The membrane stabilizing activity was also tested. The antibody response was determined by assessing the specific haemagglutination antibodies raised against sheep red blood cells. Results. The three doses of freeze-dried concoction used ((human equivalent dose (HED)—183 mg/kg) 2 × HED and 1/2HED; n = 6 rats/group) showed significant inhibition of paw edema compared to water control at 3rd–5th hours (p<0.05). Both HED and 1/2HED exhibited marked anti-inflammatory activity (72–83% inhibition at 4th-5th hours; p<0.05). The HED of the concoction showed significant inhibition of NO (77.5 ± 0.73%, p<0.001) and ROS production (26.9 ± 2.55%; p<0.01) by rat peritoneal cells. Inhibition of NO production in the concoction treated rat peritoneal cells was confirmed by the lack of iNOS expression. The concoction also exhibited significant membrane stabilizing activity (IC50 = 0.0006 μg/ml; p=0.001). HED resulted in a significantly high induction of specific antibody production against SRBC antigens as detected by SRBC haemagglutination assay (mean day 14 titers 253.3 compared to control: 66.7) (p<0.01). Conclusions. The traditional Sri Lankan concoction of C. sativum and C. fenestratum demonstrated potent in vivo anti-inflammatory activity, significant reduction of ROS, and NO production by rat peritoneal cells and the lack of iNOS expression confirmed the low NO production. The increased membrane stability also supports the anti-inflammatory activity of the concoction. Further, this concoction induced a significantly high antibody response reflecting its immunostimulatory activity. Together these results scientifically validate the therapeutic use of the concoction of C. sativum and C. fenestratum in Sri Lankan traditional medicinal system for immunomodulatory effects.

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