scholarly journals Effect of spray-drying and ultraviolet C radiation as biosafety steps for CSFV and ASFV inactivation in porcine plasma

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249935
Author(s):  
Elena Blázquez ◽  
Carmen Rodríguez ◽  
Jesús Ródenas ◽  
Rosa Rosell ◽  
Joaquim Segalés ◽  
...  
Keyword(s):  
Spray Drying ◽  
Manufacturing Process ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Storage Period ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Improve Health Status ◽  
Uv C ◽  
Spray Dried

Spray-dried animal plasma (SDAP) is widely used in diets of domestic animals to improve health status and increase growth and feed efficiency. Individual steps in the SDAP manufacturing process, including spray-drying, have been validated to inactivate potential pathogens. Manufacturing standards have established a minimum exit temperature of 80°C and a minimum post-drying storage period of 14 days at 20°C for production of SDAP. Also, UV-C irradiation has been evaluated as another inactivation step that could be included in the manufacturing process. The aim of this study was to assess the inactivation effectiveness of spray-drying on Classical swine fever virus (CSFV) and African swine fever virus (ASFV) and the effect of UV-C inactivation on ASFV as redundant biosafety steps of the manufacturing process for producing spray-dried porcine plasma (SDPP). This study demonstrated that UV-C treatment of liquid porcine plasma can inactivate more than 4 Log10 TCID50/mL of ASFV at 3000 J/L. Spray-drying effectively inactivated at least 4 Log10 TCID50/mL of both CSFV and ASFV. Incorporating UV-C technology within the SDAP manufacturing process can add another biosafety step to further enhance product safety.

scholarly journals Salt inactivation of classical swine fever virus and African swine fever virus in porcine intestines confirms the existing in vitro casings model

2019 ◽  
Vol 238 ◽  
pp. 108424 ◽  
Author(s):  
Tinka Jelsma ◽  
Joris J. Wijnker ◽  
Bregtje Smid ◽  
Eline Verheij ◽  
Wim H.M. van der Poel ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus

scholarly journals African swine fever virus infection in Classical swine fever subclinically infected wild boars

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Oscar Cabezón ◽  
Sara Muñoz-González ◽  
Andreu Colom-Cadena ◽  
Marta Pérez-Simó ◽  
Rosa Rosell ◽  
...  
Keyword(s):  
Virus Infection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Wild Boars

scholarly journals Development of A One-Step Multiplex qRT-PCR Assay For Detection of African Swine Fever Virus, Classical Swine Fever Virus And Atypical Porcine Pestivirus

2021 ◽  
Author(s):  
Huixin Liu ◽  
Kaichuang Shi ◽  
Jing Zhao ◽  
Yanwen Yin ◽  
Yating Chen ◽  
...  
Keyword(s):  
Southern China ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Differential Detection ◽  
Guangxi Province ◽  
Diarrhea Virus ◽  
Qrt Pcr ◽  
One Step

Abstract Background: African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused great economic losses to the swine industry in China. Since there exist co-infections of ASFV, CSFV and APPV in certain pig herds, it is necessary to accurately and differentially detect these pathogens in the fields. In this study, a one-step multiplex real-time quantitative reverse transcription-polymerase chain reaction (multiplex qRT-PCR) was developed for simultaneous and differential detection of ASFV, CSFV and APPV.Results: The developed one-step multiplex qRT-PCR was able to specifically detect ASFV, CSFV and APPV, but could not amplify other viruses, including porcine circovirus type 2 (PCV2), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease virus (FMDV), porcine parvovirus (PPV), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PRoV), porcine deltacoronavirus (PDCoV), border disease virus (BDV), bovine viral diarrhea virus type 1 (BVDV-1), BVDV-2 and so on. The detection limit of the assay was 2.52×101 copies/μL for ASFV, CSFV and APPV. Repeatability test using standard plasmids showed that the coefficients of variation of the intra- and inter-assay were less than 2 %. The detection of 509 clinical samples collected in Guangxi Province, Southern China from October 2018 to December 2020 showed that the positive rates of ASFV, CSFV and APPV were 45.58 %, 12.57 % and 3.54 %, respectively, while the co-infection rates of ASFV and CSFV, ASFV and APPV, CSFV and APPV were 4.91 %, 1.38 %, 0.98 %, respectively. Phylogenetic analysis based on the nucleotide sequences of partial ASFV p72 gene showed that all ASFV strains from Guangxi Province, Southern China belonged to genotype I and II.Conclusion: A one-step multiplex qRT-PCR with high specificity, sensitivity, accuracy and repeatability was successfully developed for simultaneous and differential detection of ASFV, CSFV and APPV.

A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

2015 ◽  
Vol 18 (4) ◽  
pp. 715-723 ◽  
Author(s):  
L. Hu ◽  
X.Y. Lin ◽  
Z.X. Yang ◽  
X.P. Yao ◽  
G.L. Li ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
Simultaneous Detection ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Multiple Infections ◽  
Highly Pathogenic

Abstract In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×104, 1.50×103, 2.10×103, 1.30×103 and 8.97×102 copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

scholarly journals Porcine Dendritic Cells and Viruses: An Update

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 445 ◽  
Author(s):  
Giulia Franzoni ◽  
Simon P. Graham ◽  
Silvia Dei Giudici ◽  
Annalisa Oggiano
Keyword(s):  
Dendritic Cells ◽  
Viral Infections ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Swine Industry ◽  
Emerging Viral Diseases

Several viral infections of swine are responsible for major economic losses and represent a threat to the swine industry worldwide. New tools are needed to prevent and control endemic, emerging, and re-emerging viral diseases. Dendritic cells (DC) play a central role in linking the innate and adaptive arms of the immune system, so knowledge regarding their interaction with pathogens is necessary to understand the mechanisms underlying diseases pathogenesis and protection. In the first part of this review, we provide an update on the heterogeneous cell subsets that comprise the porcine DC family. In the second part of this review, we provide an overview of how three viruses, affecting pork production at a global level, African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine circovirus 2 (PCV2), modulate DC function.

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