Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Purpose This study aims to prepare folic acid coated tin oxide nanoparticles (FA-SnO2 NPs) for specifically targeting human ovarian cancer cells with minimum side effects against normal cells. Methods The prepared FA-SnO2 NPs were characterized by FT-IR, UV-vis spectroscopy, XRD, SEM and TEM. The inhibition effects of FA-SnO2 NPs against SKOV3 cancer cell were tested by MTT and LDH assay. Apoptosis induction in FA-SnO2 NPs treated SKOV3 cells were investigated using Annexin V/PI, AO/EB and Comet assays and the possible mechanisms of the cytotoxic action were studied by Flow cytometry, qRT-PCR, Immunohistochemistry, and Western blotting analyses. The effects of FA-SnO2 NPs on reactive oxygen species generation in SKOV3 cells were also examined. Additionally, the safety of utilization FA-SnO2 NPs were studied in vivo using Wister rats. Results The obtained FA-SnO2 NPs displayed amorphous spherical morphology with an average diameter of 157 nm and a zeta potential value of -24 mV. Comparing to uncoated SnO2 NPs, FA-SnO2 NPs had a superior inhibition effect towards SKOV3 cell growth that was suggested to be mediated through higher reactive oxygen species generation. It was showed that FA-SnO2 NPs increased significantly the % of apoptotic cells in the sub- G1 and G2/M phases with a higher intensity comet nucleus in SKOV3 treated cells. Furthermore, FA-SnO2 NPs was significantly increased the expression levels of P53, Bax, and cleaved Caspase-3 and accompanied with a significant decrease of Bcl-2 in the treated SKOV3 cells. Conclusion Overall, the results suggested that an increase in cellular FA-SnO2 NPs internalization resulted in a significant induced cytotoxicity in SKOV3 cancer cells in dose-dependent mode through ROS-mediated cell apoptosis that may have occurred through mitochondrial pathway. Additionally, the results confirmed the safety of utilization FA-SnO2 NPs against living systems. So, FA-SnO2 NPs with a specific targeting moiety may be a promising therapeutic candidate for human ovarian cancer.

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Gadolinium Oxide Nanoparticles Enhance the Cytotoxicity of Chemotherapeutic Drugs by Blocking Autophagic Flux in Human Ovarian Cancer Cells

Current Nanoscience ◽

10.2174/1573413716666200313155239 ◽

2020 ◽

Vol 16 ◽

Author(s):

Zhixiong Xie ◽

Tianyu Zhang ◽

Cheng Zhong

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Hela Cells ◽

Late Stage ◽

Ovarian Cancer Cells ◽

Oxide Nanoparticles ◽

Autophagic Flux ◽

Human Ovarian Cancer ◽

Chemotherapeutic Drugs ◽

Chemotherapy Drugs

Background: During chemotherapy, drugs can damage cancer cells’ DNA and cytomembrane structure, and then induce cell death. However, autophagy can increase the chemotherapy resistance of cancer cells, reducing the effect of chemotherapy. Objective: To block the autophagic flux in cancer cells, it is vital to enhance the anti-cancer efficacy of chemotherapy drugs; for this purpose, we test the gadolinium oxide nanoparticles (Gd2O3 NPs)’ effect on autophagy. Methods: The cytotoxicity of Gd2O3 NPs on HeLa cells was evaluated by a (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, monodasylcadaverine staining, immunofluorescence, immunoblot and apoptosis assay were conducted to evaluate the effect of Gd2O3 NPs on autophagy and efficacy of chemotherapy drugs in human ovarian cancer cells. Results: We found that Gd2O3 NPs, which have great potential for use as a contrast agent in magnetic resonance imaging, could block the late stage of autophagic flux in a dose-dependent manner and then cause autophagosome accumulation in HeLa cells. When co-treated with 8 μg/mL Gd2O3 NPs and 5 μg/mL cisplatin, the number of dead HeLa cells increased by about 20% compared with cisplatin alone. We observed the same phenomenon in cisplatin-resistant COC1/DDP cells. Conclusion: We conclude that Gd2O3 NPs can block the late stage of autophagic flux and enhance the cytotoxicity of chemotherapeutic drugs in human ovarian cancer cells. Thus, the nanoparticles have significant potential for use in both diagnosis and therapy of solid tumor.

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Costunolide induces apoptosis in platinum-resistant human ovarian cancer cells by generating reactive oxygen species

Gynecologic Oncology ◽

10.1016/j.ygyno.2011.08.031 ◽

2011 ◽

Vol 123 (3) ◽

pp. 588-596 ◽

Cited By ~ 40

Author(s):

Yeong-In Yang ◽

Ji-Hyun Kim ◽

Kyung-Tae Lee ◽

Jung-Hye Choi

Keyword(s):

Reactive Oxygen Species ◽

Ovarian Cancer ◽

Cancer Cells ◽

Reactive Oxygen ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Oxygen Species ◽

Platinum Resistant

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Abstract 4175: Reactive oxygen species-dependent ERK and JNK activation is important to docasahexaenoic acid-induced cell cytotoxicity in human ovarian cancer cells

10.1158/1538-7445.am2012-4175 ◽

2012 ◽

Author(s):

Soyeon Jeong ◽

Kaipeng Jing ◽

Soyeon Shin ◽

Nayeong Kim ◽

Hye-Rim Oh ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Ovarian Cancer ◽

Cancer Cells ◽

Reactive Oxygen ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Oxygen Species ◽

Cell Cytotoxicity ◽

Jnk Activation ◽

Docasahexaenoic Acid

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The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: A comparison with the effect of doxorubicin

Toxicology in Vitro ◽

10.1016/j.tiv.2011.08.009 ◽

2011 ◽

Vol 25 (8) ◽

pp. 1712-1720 ◽

Cited By ~ 21

Author(s):

Aneta Rogalska ◽

Arkadiusz Gajek ◽

Marzena Szwed ◽

Zofia Jóźwiak ◽

Agnieszka Marczak

Keyword(s):

Reactive Oxygen Species ◽

Ovarian Cancer ◽

Cancer Cells ◽

Reactive Oxygen ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Oxygen Species

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Small Interfering RNA-Mediated Silencing of Heat Shock Protein 27 (HSP27) Increases Chemosensitivity to Paclitaxel by Increasing Production of Reactive Oxygen Species in Human Ovarian Cancer Cells (HO8910)

Journal of International Medical Research ◽

10.1177/147323000903700512 ◽

2009 ◽

Vol 37 (5) ◽

pp. 1375-1388 ◽

Cited By ~ 27

Author(s):

TF Song ◽

ZF Zhang ◽

L Liu ◽

T Yang ◽

J Jiang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Ovarian Cancer ◽

Heat Shock ◽

Heat Shock Protein ◽

Cancer Cells ◽

Small Interfering Rna ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Oxygen Species ◽

Interfering Rna

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Effects of nano-taxol/curcumin on ovarian cancer cells

Materials Express ◽

10.1166/mex.2020.1818 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1615-1619

Author(s):

Shuai Zhang ◽

Junhui Liang ◽

Changzhong Li ◽

Fei Wang

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Culture Medium ◽

Morphological Characteristics ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Pharmacodynamic Effect ◽

Skov3 Cells ◽

Inhibit Cell Proliferation

To investigate the pharmacodynamic effect of urushin nanoparticles upon the proliferation inhibition in human ovarian cancer SKOV3 cells, and in order to explore their biomechanism, the cell cycle and the percentage of apoptotic cells in human ovarian cancer SKOV3 cells were analyzed utilizing flow cytometry. The concentration of astragalin nanoparticles in SKOV3 cells was identified utilizing HPIC. Consequently, the morphological characteristics of SKOV3 cells in a culture medium of 5 mg/L were investigated and measured. In our findings, the 50 mg cancer cells containing 50 mg IC did not display this noted effect. The results exhibit the discovery that urushin nanoparticles inhibit cell proliferation, which is related to the inhibition of DNA replication and the regulation of the cell proliferation cycle. HPLC results demonstrated that the pharmacological effect of urushin nanoparticles was directly related to the drug concentration present within the studied cells. Hence, urushin nanoparticles can effectively enter cells and then effectively inhibit cell proliferation.

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