scholarly journals Qualitative lysine crotonylome analysis in the ovarian tissue of Harmonia axyridis (Pallas)

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258371
Author(s):  
Changying Zheng ◽  
Lijuan Sun
Keyword(s):  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
Protein Biosynthesis ◽  
Ovarian Tissue ◽  
Control Agent ◽  
Female Fertility ◽  
Ovary Development ◽  
Common Mechanism ◽  
Cellular Processes ◽  
Development Regulation

Lysine crotonylation (Kcr) is a newly discovered posttranslational modification (PTM), which has been studied at the proteomics level in a few species, with the study of Kcr in female fertility and in insect species is still lacking. Harmonia axyridis (Pallas) is a well-known beneficial insect used as a natural biological control agent against aphids in agriculture. Here, global Kcr identification in ovarian tissue of H. axyridis at diapause stage was performed to reveal potential roles for Kcr in H. axyridis ovarian cellular processes, female fertility and diapause regulation. In total, 3084 Kcr sites in 920 proteins were identified. Bioinformatic analyses revealed the distribution of these proteins in multiple subcellular localization categories and their involvement in diverse biological processes and metabolism pathways. Carbohydrate and energy metabolism related cellular processes including citric acid cycle, glycolysis and oxidative phosphorylation appeared be affected by Kcr modification. In addition, regulation of translation and protein biosynthesis may reflect Kcr involvement in diapause in H. axyridis, with Kcr affecting ribosome activities and amino acid metabolism. Moreover, Kcr modulation H. axyridis ovary development regulation may share some common mechanism with Kcr participation in some disease progression. These processes and pathways were uncovered under diapause stage, but possibly not enriched/specific for diapause stage due to limitations of qualitative proteomics experimental design. Our results informs on the potential for Kcr modifications to regulate female fertility and insect physiology.

scholarly journals Harmonine, a defence compound from the harlequin ladybird, inhibits mycobacterial growth and demonstrates multi-stage antimalarial activity

2011 ◽  
Vol 8 (2) ◽  
pp. 308-311 ◽  
Author(s):  
Christian Rene Röhrich ◽  
Che Julius Ngwa ◽  
Jochen Wiesner ◽  
Henrike Schmidtberg ◽  
Thomas Degenkolb ◽  
...  
Keyword(s):  
Harmonia Axyridis ◽  
Antimalarial Activity ◽  
Biological Control Agent ◽  
Control Agent ◽  
Human Pathogens ◽  
Harlequin Ladybird ◽  
Multi Stage ◽  
Blocking Activity ◽  
Mycobacterial Growth ◽  
Gametocytocidal Activity

The harlequin ladybird beetle Harmonia axyridis has been introduced in many countries as a biological control agent, but has become an invasive species threatening the biodiversity of native ladybirds. Its invasive success has been attributed to its vigorous resistance against diverse pathogens. This study demonstrates that harmonine ((17 R ,9 Z )-1,17-diaminooctadec-9-ene), which is present in H. axyridis haemolymph, displays broad-spectrum antimicrobial activity that includes human pathogens. Antibacterial activity is most pronounced against fast-growing mycobacteria and Mycobacterium tuberculosis , and the growth of both chloroquine-sensitive and -resistant Plasmodium falciparum strains is inhibited. Harmonine displays gametocytocidal activity, and inhibits the exflagellation of microgametocytes and zygote formation. In an Anopheles stephensi mosquito feeding model, harmonine displays transmission-blocking activity.

Walking Activity of Flightless Harmonia axyridis (Coleoptera: Coccinellidae) as a Biological Control Agent

2010 ◽  
Vol 103 (5) ◽  
pp. 1564-1568 ◽  
Author(s):  
Satoshi Nakayama ◽  
Tomokazu Seko ◽  
Jun-Ichi Takatsuki ◽  
Kazuki Miura ◽  
Takahisa Miyatake
Keyword(s):  
Biological Control ◽  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
Control Agent ◽  
Walking Activity

scholarly journals Optimizing Sample Size for Population Genomic Study in a Global Invasive Lady Beetle, Harmonia Axyridis

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 290 ◽  
Author(s):  
Hongran Li ◽  
Wanmei Qu ◽  
John J. Obrycki ◽  
Ling Meng ◽  
Xuguo Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sample Size ◽  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
Control Agent ◽  
Lady Beetle ◽  
Accurate Estimation ◽  
Nucleotide Polymorphisms ◽  
Population Genomic ◽  
Genomic Study

Finding optimal sample sizes is critical for the accurate estimation of genetic diversity of large invasive populations. Based on previous studies, we hypothesized that a minimal sample size of 3–8 individuals is sufficient to dissect the population architecture of the harlequin lady beetle, Harmonia axyridis, a biological control agent and an invasive alien species. Here, equipped with a type IIB endonuclease restriction site-associated (2b-RAD) DNA sequencing approach, we identified 13,766 and 13,929 single nucleotide polymorphisms (SNPs), respectively, among native and invasive H. axyridis populations. With this information we simulated populations using a randomly selected 3000 SNPs and a subset of individuals. From this simulation we finally determined that six individuals is the minimum sample size required for the accurate estimation of intra- and inter-population genetic diversity within and across H. axyridis populations. Our findings provide an empirical advantage for population genomic studies of H. axyridis in particular and suggest useful tactics for similar studies on multicellular organisms in general.

scholarly journals Influence of Elytral Colour Pattern, Size, and Sex of Harmonia axyridis (Coleoptera, Coccinellidae) on Parasite Prevalence and Intensity of Hesperomyces virescens (Ascomycota, Laboulbeniales)

2018 ◽  
Author(s):  
Danny Haelewaters ◽  
Thomas Hiller ◽  
Michał Gorczak ◽  
Donald H. Pfister
Keyword(s):  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
Parasite Prevalence ◽  
Control Agent ◽  
Colour Pattern ◽  
Distribution Area ◽  
Fruiting Bodies ◽  
Intensity Of Infection ◽  
Pattern Size ◽  
Colour Patterns

Harmonia axyridis is an invasive ladybird (Coleoptera, Coccinellidae) with the potential to outcompete native ladybird species in its invasive distribution area. It was introduced as a biological control agent in many countries but has also spread unintentionally in many others. Hesperomyces virescens (Ascomycota, Laboulbeniales) is a minute (200–400 µm in size) biotrophic fungus that infects over 30 species of ladybirds. The aim of this study was to evaluate whether elytral colour pattern, size, and sex of Ha. axyridis affect infection by H. virescens. Colouration in Ha. axyridis has been linked to the presence of an antimicrobial alkaloid (harmonine). In fall 2016, we collected 763 Ha. axyridis individuals in Cambridge, Massaschusetts, of which 119 (16%) bore H. virescens fruiting bodies. We analysed 160 individuals concerning prevalence and intensity of infection by H. virescens. Elytral sizes and colouration patterns were quantified using digital photography and analytical methods. Smaller ladybirds had a higher prevalence and higher intensity of parasitism. Additionally, male ladybirds bore more thalli compared to female ladybirds. Elytral colour patterns had an effect on neither prevalence nor intensity of infection by Laboulbeniales in our dataset. This suggests that development of Laboulbeniales may be unaffected by certain insect alkaloids.

scholarly journals What has Harmonia axyridis to do with Dante's Divine Comedy

2010 ◽  
pp. 82-88
Author(s):  
András Bozsik
Keyword(s):  
Western Europe ◽  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
National Level ◽  
Control Agent ◽  
Classical Literature ◽  
Wine Quality ◽  
The Usa ◽  
Animal Names ◽  
Horticultural Pest

The multicoloured Asian ladybird beetle (Harmonia axyridis (Pallas, 1773)), a former successful biological control agent in the USA and Western Europe became an invasive alien species threatening the diversity of native aphidophagous insects through competition and praying, a horticultural pest consuming various fruits and adversely affecting the wine quality and a human nuisance when occurring at high densities in buildings. Due to this dubious fame, attention has been paid to it also at national level which made inevitable to find for it a fitting name in different languages. In some cases and countries a version of the English name has been chosen however, regarding the meaning of this translated term in Hungarian, the try cannot be called fortunate. The objective of this contribution is to stress the function of common animal names in the Hungarian Sprachraum and to find a right and proper alternative with the help of etymology, mythology, classical literature, art (painting) and the experiences of Hungarian fruit and grape growers.

scholarly journals PRVA NAJDBA OGORČIC PARASITYLENCHUS BIFURCATUS POINAR & STEENBERG, 2012, PARAZITOV PISANE POLONICE HARMONIA AXYRIDIS (PALLAS, 1773) V SLOVENIJI

2017 ◽  
Vol 109 (2) ◽  
pp. 457 ◽  
Author(s):  
Barbara Gerič Stare ◽  
Saša ŠIRCA ◽  
Gregor UREK
Keyword(s):  
Harmonia Axyridis ◽  
Biological Control Agent ◽  
Nematode Species ◽  
Control Agent ◽  
High Similarity ◽  
The Public ◽  
Characteristic Features ◽  
Tail Tip ◽  
Juvenile Males ◽  
Harmonia Axyridis Pallas

In years 2015 and 2016 around 200 specimens of ladybird species <em>Harmonia axyridis</em> were collected in Slovenia. Ladybirds were dissected and checked under stereomicroscope for presence of nematodes. Nematodes were discovered in samples of ladybirds <em>H. axyridis</em> collected at Brdo pri Lukovici and Trboje. The incidence of parasitized ladybirds was around 30 %. Nematodes were analysed morphometrically and the characteristic features of <em>Parasitylenchus bifurcatus</em> nematodes were observed: straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform females and juvenile males. The identity was confirmed with 18S rDNA region sequence (acc. no. LT629306 and LT629307) which showed high similarity (&gt;99.9 % nucleotide identity) to the <em>P. bifurcatus</em> sequences in the public domain. This is a first report of <em>P. bifurcatus </em>nematode species found parasitizing ladybird <em>Harmonia axyridis</em> in Slovenia. The species compromises<em> </em>fecundity of its host and has therefore a potential to be used as a biological control agent to control high abundance of invasive <em>H. axyridis</em> ladybirds<em>.</em>

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