Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review

Background and Aim: Spermatogonial stem cells (SSCs) have previously been isolated from animals' testes, cultured in vitro, and successfully transplanted into compatible recipients. The SSC unique characteristic has potential for exploitation as a reproductive tool and this can be achieved through SSC intratesticular transplantation to surrogate sires. Here, we aimed at comprehensively analyzing published data on in vitro maintenance of SSC isolated from the testes of livestock animals and their applications. Materials and Methods: The literature search was performed in PubMed, Science Direct, and Google Scholar electronic databases. Data screening was conducted using Rayyan Intelligent Systematic Review software (https://www.rayyan.ai/). Duplicate papers were excluded from the study. Abstracts were read and relevant full papers were reviewed for data extraction. Results: From a total of 4786 full papers screened, data were extracted from 93 relevant papers. Of these, eight papers reported on long-term culture conditions (>1 month) for SSC in different livestock species, 22 papers on short-term cultures (5-15 days), 10 papers on transfection protocols, 18 papers on transplantation using different methods of preparation of livestock recipients, and five papers on donor-derived spermatogenesis. Conclusion: Optimization of SSC long-term culture systems has renewed the possibilities of utilization of these cells in gene-editing technologies to develop transgenic animals. Further, the development of genetically deficient recipients in the endogenous germline layer lends to a future possibility for the utilization of germ cell transplantation in livestock systems.

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Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells

Human Reproduction ◽

10.1093/humrep/dex348 ◽

2017 ◽

Vol 33 (1) ◽

pp. 81-90 ◽

Cited By ~ 15

Author(s):

Callista L Mulder ◽

Lisa A E Catsburg ◽

Yi Zheng ◽

Cindy M de Winter-Korver ◽

Saskia K M van Daalen ◽

...

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells

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Long-Term Culture and Transplantation of Spermatogonial Stem Cells from BALB/c Mice

Cells Tissues Organs ◽

10.1159/000276586 ◽

2010 ◽

Vol 191 (5) ◽

pp. 372-381 ◽

Cited By ~ 5

Author(s):

Fujin Shen ◽

Ci Zhang ◽

Hongyun Zheng ◽

Yunhe Xiong ◽

Xi Wang ◽

...

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells ◽

Long Term Culture

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Dantrolene in the treatment of MDMA-related hyperpyrexia: a systematic review

CJEM ◽

10.1017/s1481803500012598 ◽

2010 ◽

Vol 12 (05) ◽

pp. 435-442 ◽

Cited By ~ 38

Author(s):

Brian E. Grunau ◽

Matthew O. Wiens ◽

Jeffrey R. Brubacher

Keyword(s):

Systematic Review ◽

Clinical Decision Making ◽

Case Reports ◽

Data Extraction ◽

Patient Characteristics ◽

Clinical Decision ◽

Poison Control ◽

Published Data ◽

Published Evidence

ABSTRACTObjective:The use of dantrolene in the treatment of hyperpyrexia related to MDMA (3,4-methylenedioxymethamphetamine) is controversial, with little data available to guide clinical decision-making. Although the treatment is recommended by several poison control centres, published data are primarily in the form of case reports and animal and in vitro experiments. We conducted a systematic review to investigate the published evidence regarding the safety and benefits of dantrolene for MDMA-related hyperpyrexia in humans.Data sources:A systematic search of Embase and MEDLINE was conducted from the earliest possible date to November 2008.Study selection:All human trials and case reports of MDMA-related hyperpyrexia were considered.Data extraction:Data were abstracted systematically and characteristics including use of dantrolene, adverse reactions attributed to dantrolene, peak temperature, complications from MDMA-related hyperpyrexia and survival were recorded.Data synthesis:Our search yielded 668 articles of which 53, reporting 71 cases of MDMA-induced hyperpyrexia, met our inclusion criteria. No clinical trials, randomized controlled trials, observational studies or meta-analyses were identified. Dantrolene was used in 26 cases. Patient characteristics were similar in the dantrolene and no dantrolene groups. The proportion of survivors was higher in the dantrolene group (21/26) than in the no dantrolene group (25/45). This difference was especially pronounced in those with extreme (≥ 42°C) and severe (≥ 40°C) fever, with a survival rate of 8 of 13 and 10 of 10, respectively, in the dantrolene group compared with 0 of 4 and 15 of 27 in the no dantrolene group. There were no reports of adverse events attributable to dantrolene with the exception of a possible association with an episode of transient hypoglycemia.Conclusion:Our systematic review suggests that dantrolene is safe for patients with MDMA-related hyperpyrexia. Dantrolene may also be associated with improved survival and reduced complications, especially in patients with extreme (≥ 42°C) or severe (≥ 40°C) hyperpyrexia, although this conclusion must be interpreted with caution given the risk of reporting or publication bias.

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Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

Cellular Reprogramming ◽

10.1089/cell.2016.0056 ◽

2017 ◽

Vol 19 (3) ◽

pp. 159-170 ◽

Cited By ~ 3

Author(s):

Agnese Gugliandolo ◽

Thangavelu Soundara Rajan ◽

Domenico Scionti ◽

Francesca Diomede ◽

Placido Bramanti ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Culture In Vitro ◽

Long Term Culture ◽

Oncogene Expression

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Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways

Experimental Biology and Medicine ◽

10.1177/1535370215590822 ◽

2015 ◽

Vol 240 (8) ◽

pp. 1112-1122 ◽

Cited By ~ 26

Author(s):

Ying Guo ◽

Linhong Liu ◽

Min Sun ◽

Yanan Hai ◽

Zheng Li ◽

...

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells ◽

Long Term Culture

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qPCR analysis of mesenchymal stem cell marker expression during the long-term culture of canine adipocyte derived stem cells

Medical Journal of Cell Biology ◽

10.2478/acb-2020-0017 ◽

2020 ◽

Vol 8 (4) ◽

pp. 139-145

Author(s):

Rut Bryl ◽

Claudia Dompe ◽

Maurycy Jankowski ◽

Katarzyna Stefańska ◽

Afsaneh Golkar Narenji ◽

...

Keyword(s):

Stem Cells ◽

Molecular Mechanisms ◽

Cultured Cells ◽

Stem Cell Marker ◽

Pcr Analysis ◽

Marker Genes ◽

Long Term Culture ◽

Marker Expression

AbstractDue to its availability and accessibility, adipose tissue has been the subject of various studies in many different medical fields and is believed to be a useful source of stem cells. The ability of ASCs to differentiate towards different cell lineages, with possibility of directing this differentiation, increases their possible clinical applications, and they have been widely employed in multiple therapies and treatment of different pathologies. However, a deeper understanding of the molecular mechanisms underlying the ASCs osteoblastic and chondrocyte differentiation may lead to novel applications treating a multitude of different bone-related diseases through techniques more likely meeting worldwide consensus. In this study, the RT-qPCR method was used to determine the changes in expression of ASC specific markers (CD105, CD73, CD14, CD34, CD90 and CD45) before and after long-term (14-day) in vitro cultures. To confirm the identity of the investigated cells, flow cytometry was used to evaluate the presence of positive (CD44, CD90) and negative (CD45, CD34) ASC markers. Overall, the results of the PCR analysis showed a significant change in expression of most of the marker genes, indicating significant changes in the cultured cells caused by their long-term culture, potentially altering their original stem-like characteristics.Running title: ASC marker expression during long-term in vitro culture

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Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture

Fertility and Sterility ◽

10.1016/j.fertnstert.2014.08.022 ◽

2014 ◽

Vol 102 (6) ◽

pp. 1700-1707.e1 ◽

Cited By ~ 34

Author(s):

Bita Nickkholgh ◽

S. Canan Mizrak ◽

Saskia K.M. van Daalen ◽

Cindy M. Korver ◽

Hooman Sadri-Ardekani ◽

...

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells ◽

Long Term Culture ◽

Genetic And Epigenetic Stability

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229 LONG-TERM PROPAGATION AND CRYOPRESERVATION OF CAT SPERMATOGONIAL STEM CELLS

Reproduction Fertility and Development ◽

10.1071/rdv28n2ab229 ◽

2016 ◽

Vol 28 (2) ◽

pp. 246

Author(s):

L. M. Vansandt ◽

M. Dickson ◽

R. Zhou ◽

L. Li ◽

B. S. Pukazhenthi ◽

...

Keyword(s):

Stem Cells ◽

Germ Cell ◽

Germ Cells ◽

Spermatogonial Stem Cells ◽

Domestic Cat ◽

Feeder Cell ◽

Cell Clusters ◽

Fetal Fibroblasts

Spermatogonial stem cells (SSC) are unique adult stem cells that reside within the seminiferous tubules of the testis. As stem cells, SSC maintain the ability to self-replicate, providing a potentially unlimited supply of cells and an alternate source for preservation of the male genome. While self-renewing, long-term SSC culture has been achieved in mice, there is virtually no information regarding culture requirements of felid SSC. Therefore, the objectives of this study were to (1) evaluate the ability of 3 feeder cell lines to support germ cell colony establishment in domestic cats (Felis catus), and (2) assess long-term culture using the best feeder(s). Cells isolated enzymatically from peripubertal cat testes (n = 4) and enriched by differential plating were cultured on mouse embryonic fibroblasts (STO line), mouse-derived C166 endothelial cells, and primary cat fetal fibroblasts (cFF). Colony morphology was assessed every other day and immunocytochemistry (ICC) was performed to investigate expression of SSC markers. At 5 days in vitro (DIV), a cluster forming activity assay was used to estimate the number of SSC supported by each feeder cell line. Differences among treatments were compared using Tukey-Kramer adjustment for pair-wise mean comparisons. Data were expressed as mean cluster number ± SE per 105 cells input. When cultured on STO feeders, cat germ cells were distributed as individual cells. On both C166 cells and cFF feeders, germ cell clumps (morphologically consistent with SSC colonies in other species) were observed. Immunocytochemistry revealed that the single germ cells present on STO feeders were positive for UCHL1 and weakly expressed PLZF and OCT4. Cells within the germ cell clumps on C166 cells and cFF co-expressed all 3 SSC markers. The C166 cells supported a higher number of germ cell clusters (77.4 ± 13.8) compared with STO (3.5 ± 1.1, P = 0.0003) or cFF (22.7 ± 1.0, P = 0.0024). Therefore, subsequent subculture experiments were performed exclusively with C166 feeder layers. Cultures from 2 donors were passaged at 12 DIV and periodically as needed thereafter. Germ cell clumps consistently reestablished following each subculture and immunocytochemistry analysis confirmed maintenance of all 3 SSC markers. Cells were also positive for alkaline phosphatase activity. Cells that had been cryopreserved in culture medium with 5% (vol/vol) dimethyl sulphoxide after144 DIV (7 passages) were thawed and cultured for an additional 18 days. These cells continued to express SSC markers and form germ cell clusters. Taken together, these data demonstrate that C166 feeder cells can facilitate colony establishment and in vitro propagation of germ cell clumps in the domestic cat. This represents an important first step towards attainment and optimization of a long-term SSC culture system in the cat. This system would provide a mechanism to explore regulation of spermatogenesis, test species-specific drugs, and produce transgenic biomedical models.

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