Determination of Fatty Acid and Essential Oil Constituents and Biological Activities on Ranunculus pedatus Subsp. pedatus

Introduction: The presence of microbes in our environment is always a permanent public health problem. In this context, research on natural treatment, less expensive and accessible to fight these microbial germs would be beneficial. Methods: During this work, molecules of thiosemicarbazones due to their numerous biological activities were hemi-synthesized in situ in the essential oil of Cymbopogon citratus in order to evaluate their antimicrobial activities. Results: Analysis of the essential oil extracted by hydrodistillation revealed the presence of 72.91% of citral. Citralthiosemicarbazone (CThio) and citral 4-phenyl-3-thiosemicarbazone (CPthio) were hemi-synthesized in this oil with interesting yields of 83% and 91%, respectively. After purification and confirmation of the structures of these molecules, the three substances were tested on eleven strains of microbes. Determination of the inhibition diameters showed that the activity of the essential oil is best in over 80% of strains. However, the largest diameter of inhibition (26 mm) was noted with CPthio against Salmonella typhi R 30951401. The determination of the minimum inhibitory concentrations showed that the oil remains more active with the smallest value of 0.3125 mg / ml against Micrococcus luteus. The lower value of minimum bactericidal concentrations was also obtained with the essential oil against Enterococcus foecalis ATCC 29212. Conclusion: The essential oil of C. citratus remains more active in the majority cases. It could be a great alternative in the fight against bacteria, and the advantage is that it remains a natural substance.

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Gas Chromatographic/Mass Spectrometric Analysis of the Essential Oil of Houttuynia cordata Thunb by Using On-Column Methylation with Tetramethylammonium Acetate

Journal of AOAC International ◽

10.1093/jaoac/90.1.60 ◽

2007 ◽

Vol 90 (1) ◽

pp. 60-67 ◽

Cited By ~ 9

Author(s):

Muhammad Ishtiaq Ch ◽

Yang F Wen ◽

YiYu Cheng

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Essential Oil ◽

Capric Acid ◽

Inlet Temperature ◽

Good Efficiency ◽

Houttuynia Cordata ◽

Acid Methyl ◽

Houttuynia Cordata Thunb

Abstract This paper describes a simple and novel on-column derivatization procedure used with gas chromatography/mass spectrometry (GC/MS) for the analysis of essential oil of Houttuynia cordata Thunb (HCT), a traditional Chinese medicine. In the procedure, the essential oil was obtained by hydrodistillation, and the fatty acid components were derivatized with tetramethylammonium acetate (TMAA) at 250°C and identified by GC/MS. Methylation improved the determination of both the fatty acids and the other components in the essential oil of HCT. To obtain optimum methylation conditions, several important factors were investigated with pentadecane as the internal standard and a GC inlet temperature of 250°C. Tetramethylammonium hydroxide (TMAH) and TMAA were compared as the derivatization agent, and a 2:1 ratio of TMAA to capric acid was evaluated. Fatty acid methyl esters produced good chromatographic peak shapes and did not interfere with the determination of dodecanal and caryophyllene. TMAA is a neutral methylation reagent, and it yielded no side reactions during derivatization. It was found that the fatty acid content of the essential oil was about 81%; among the methylated fatty acids found were capric acid, methyl (43.66%), methyl laurate (16.15%), methyl hexadecanoate (9.27%), undecanoic acid, methyl (5.62%), methyl oleate (1.98%), and methyl linoleate (1.40%). Other major constituents were (-)-β-pinene (1.02%), β-myrcene (1.62%), 1-terpinen-4-ol (1.59%), decanal (1.49%), and 2-undecanone (1.47%). The results obtained demonstrated good efficiency for the procedure. Pure chromatograms allowed quantitation, which was obtained by total volume integration. The on-column derivatization procedure was simple to perform, and it improved the sensitivity, the peak resolution, and the selectivity of the GC/MS determination.

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Chemical composition and antibacterial effect of Smyrnium olusatrum L. Fruit Essential Oil

Mediterranean Journal of Chemistry ◽

10.13171/mjc10602006231292iz ◽

2020 ◽

Vol 10 (6) ◽

pp. 577

Author(s):

Saida Cherif Hamida ◽

Imane Zalegh ◽

Fairouz Saidi ◽

Nabahat Benmanssour ◽

M. Carmen González-Mas ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antibacterial Effect ◽

Biological Activities ◽

Inhibition Zone ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Therapeutic Properties

The plants of the Apiaceae family are mainly used for food purposes and their multiple therapeutic properties and biological activities. This study aims to determine the chemical composition of Essential Oil (EO) of the fruits of Smyrnium olusatrum L. obtained by hydrodistillation in Clevenger-type apparatus. GC/MS analyzed the EO and the antibacterial activity was evaluated against clinical bacterial strains by two complementary methods (Disc diffusion and micro-atmosphere) and determination of Minimal Inhibitory Concentration (MIC). The results of chemical composition show that the EO is dominated by hydrocarbon monoterpenes (55,95%). The antibacterial effect shows that Gram-positive bacteria are the most sensitive to the inhibitory action of EO with inhibition zone diameters (DZI) ranging from 18 to 20 mm for Staphylococcus aureus and Streptococcus faecium strains. The same results were reported with the micro-atmosphere

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Determination of antioxidant, cytotoxic, anticholinesterase, antiurease, antityrosinase, and antielastase activities and aroma, essential oil, fatty acid, phenolic, and terpenoid-phytosterol contents of Salvia poculata

Industrial Crops and Products ◽

10.1016/j.indcrop.2020.112712 ◽

2020 ◽

Vol 155 ◽

pp. 112712

Author(s):

Ismail Yener

Keyword(s):

Fatty Acid ◽

Essential Oil

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Method validation of 15 phytochemicals in Hypericum lysimachioides var. spathulatum by LC–MS/MS, and fatty acid, essential oil, and aroma profiles with biological activities

Journal of Food Measurement & Characterization ◽

10.1007/s11694-020-00562-6 ◽

2020 ◽

Vol 14 (6) ◽

pp. 3194-3205

Author(s):

Mehmet Akdeniz ◽

Mustafa Abdullah Yilmaz ◽

Abdulselam Ertas ◽

Ismail Yener ◽

Mehmet Firat ◽

...

Keyword(s):

Fatty Acid ◽

Essential Oil ◽

Method Validation ◽

Biological Activities

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Determination of biological activities of essential oil and extract obtained from Achillea coarctata Poir.

Advances in Traditional Medicine ◽

10.1007/s13596-019-00378-w ◽

2019 ◽

Vol 20 (1) ◽

pp. 77-88 ◽

Cited By ~ 1

Author(s):

Sevil Albayrak ◽

Nurcan Silahtarlıoğlu

Keyword(s):

Essential Oil ◽

Biological Activities

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Simultaneous Determination of Essential Oil Components and Fatty Acids in Fennel using Gas Chromatography with a Polar Capillary Column

Natural Product Communications ◽

10.1177/1934578x1501000933 ◽

2015 ◽

Vol 10 (9) ◽

pp. 1934578X1501000 ◽

Cited By ~ 2

Author(s):

Menče Najdoska-Bogdanov ◽

Jane B. Bogdanov ◽

Marina Stefova

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Essential Oil ◽

Simultaneous Determination ◽

Soxhlet Extraction ◽

Volatile Fraction ◽

Foeniculum Vulgare ◽

Oil Components ◽

Main Components

Cultivated and wild growing samples of fennel ( Foeniculum vulgare Mill., Apiaceae) from R. Macedonia were studied for their volatiles and fatty acid composition. The main essential oil components isolated via hydrodistillation were: trans-anethole (>80%), estragole (<6%), limonene (<6%), anisaldehyde (<1%) and 0.5 % fenchone. An alternative method for characterization of both the non-polar volatile and non volatile fractions was developed using n-hexane and dichloromethane (3:1, v/v) in a Soxhlet extraction followed by transesterification. The obtained extracts were then characterized and the dominant fatty acid was 18:1 (petroselinic and oleic acid) 75.0–82.8 %, followed by 18:2 (linoleic acid) 10.8–16.2% and other fatty acids: palmitic (4.3–6.9%), stearic (1.2–1.7%) and myristic (0–2.9%). The results for the volatile fraction after Soxhlet extraction and transesterification did not significantly differ from results obtained after hydrodistillation, especially for the main components ( trans-anethole, estragole, fenchone and limonene), implying that the developed method can be used for simultaneous determination of volatiles and fatty acids.

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