PROCESSING OF BITTER ALMOND KERNELS AND OBTAINING EXTRACTS BASED ON THEM

Mountain almond kernels (seed) processing products are of practical interest as fungicidal, antimicrobial and antiviral substances, and also find application in medicine and cosmetics for various purposes. The composition and some technological properties of seeds of mountain almond seed (Amygdalus communis L. varietas amara DC.). Cultivated in the mountain zones (Bostanlyq) of Uzbekistan were studied. To obtain seeds from cold pressing, fatty oil was separated. The cake was degreased with organic solvents, the residues were dried and ground. Using gas-liquid chromatography, the presence of 3.24% amygdalin in the composition of crushed powder of bitter almonds was established. Spectral analysis of bitter almond powder showed the presence of Mg, P, Ca, K, Si, Sr, Fe, Mn, B, Cu and other elements. The nitrogen content of the powder determined by the Dume method was 5.72 (±0.2)%. Aqueous and ethanol extracts were obtained from bitter almond powder with a yield of 10.5 and 13.2%. Chromatographic analysis of the composition of the extracts showed the presence of 0.0029% amygdalin in aqueous and 27.2% in ethanol extracts. Protein with a yield of 35.25±0.2% was isolated from the studied powder of bitter almonds. The protein is purified by dialysis, centrifugation and identified by IR spectroscopy. The amino acid analysis of the composition of the hydrolyzate protein of bitter almonds showed the presence of glycine, aspartic acid, arginine, glutamine, alanine and other amino acids. Bitter almond seed powder, extracts and protein isolated from it are of practical interest for cosmetology practice.

Download Full-text

Analysis of all protein amino acids as their tert.-butyldimethylsilyl derivatives by gas-liquid chromatography

Analytical Biochemistry ◽

10.1016/0003-2697(87)90109-6 ◽

1987 ◽

Vol 163 (1) ◽

pp. 175-181 ◽

Cited By ~ 16

Author(s):

Chin-Joo Goh ◽

Kenneth G. Craven ◽

James R. Lepock ◽

Erwin B. Dumbroff

Keyword(s):

Amino Acids ◽

Liquid Chromatography ◽

Gas Liquid Chromatography ◽

Protein Amino Acids

Download Full-text

Chiral gas chromatographic analysis of amino acids in fortified wines

Chromatographia ◽

10.1007/bf02492104 ◽

2003 ◽

Vol 57 (S1) ◽

pp. S207-S212 ◽

Cited By ~ 15

Author(s):

R. Pätzold ◽

A. Nieto-Rodriguez ◽

H. Brückner

Keyword(s):

Amino Acids ◽

Chromatographic Analysis ◽

Gas Chromatographic Analysis ◽

Fortified Wines

Download Full-text

Physicochemical Properties and Chemical Constituents of Oil from Joan Seed (Trachyspermum ammi L)

Journal of Environmental Science and Natural Resources ◽

10.3329/jesnr.v5i2.14571 ◽

2013 ◽

Vol 5 (2) ◽

pp. 15-21

Author(s):

S Morshed ◽

MK Alam ◽

A Begum ◽

SMS Shahriar ◽

KN Sharmin ◽

...

Keyword(s):

Fatty Acid ◽

Chemical Constituents ◽

Petroselinic Acid ◽

Acid Value ◽

Chemical Characteristic ◽

Local Market ◽

Gas Liquid Chromatography ◽

Fatty Oil ◽

Trachyspermum Ammi ◽

Soxhlet Apparatus

The paper deals with the investigation of the fatty oil of carom seed (Trachyspermum ammi) for its physicochemical characteristics and fatty acid composition. Oil from carom seed collected from local market was extracted with n-hexane in a glass Soxhlet apparatus. Extracted oil was dark green, burning taste, spicy in odour and soluble in n-hexane, chloroform, pet. ether and benzene. Its chemical characteristic such as acid value (6.69), Iodine value (79.39), Peroxide value (457.11), Saponification value (184.32), Unsaponified matter (9.11), Free fatty acid value as petroselinic acid (48.1) were determined by conventional method. The composition of fatty oil was determined by Gas Liquid Chromatography (GLC). From GLC analysis the main constituents of fatty oil such as Petroselinic acid (89.35%), Oleic acid (5.86%), Linoleic acid (4.79%) were determined. For the better evaluation of these spices, it is necessary to know their complete chemical composition.DOI: http://dx.doi.org/10.3329/jesnr.v5i2.14571 J. Environ. Sci. & Natural Resources, 5(2): 15-21 2012

Download Full-text

A Micromethod for the Clinical Chemistry Routine Analysis of Amino Acids in Blood and Urine by Capillary Gas Liquid Chromatography of Isobutyl Esters N(O) -Heptafluorobutyrate Derivatives

Recent Developments in Mass Spectrometry in Biochemistry and Medicine ◽

10.1007/978-1-4613-3018-9_29 ◽

1979 ◽

pp. 377-400 ◽

Cited By ~ 4

Author(s):

J. Desgres ◽

D. Boisson ◽

F. Veyrac ◽

M. Susse ◽

P. Padieu

Keyword(s):

Amino Acids ◽

Liquid Chromatography ◽

Clinical Chemistry ◽

Routine Analysis ◽

Gas Liquid Chromatography

Download Full-text

Antimicrobial Activity and Polyphenol Profiles of Hydroalcoholic Extracts of Thymus rasitatus Klokov and Thymus eremita Klokov

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6117 ◽

2021 ◽

Vol 9 (A) ◽

pp. 313-317

Author(s):

Svetlana Ivasenko ◽

Ainur Zhumabekova ◽

Agnieszka Ludwiczuk ◽

Krystyna Skalicka–Wozniak ◽

Alexandr Marchenko ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chromatographic Analysis ◽

Antimicrobial Agents ◽

Raw Materials ◽

Wide Spectrum ◽

Raw Material ◽

Thymus Vulgaris ◽

Material Base ◽

Ultrasound Assisted Extraction ◽

Ethanol Extracts

BACKGROUND: A possible reduction in stocks of medicinal plant raw materials of Thymus serpyllum L. and Thymus vulgaris L. leads to the need to expand the raw material base of the official medicinal plants with using of endemic species of the flora of Kazakhstan, in particular, Thymus rasitatus Klokov, and Thymus eremita Klokov. AIM: The aim of the study was to study the possibility of using 70% ethanol extracts of T. rasitatus and T. eremita as antimicrobial agents. MATERIALS AND METHODS: The aerial parts of T. rasitatus and T. eremita were extracted with 70% ethanol using ultrasound assisted extraction. The qualitative and quantitative analyses of the 70% ethanol extracts were determined using the liquid chromatography-detection-ESI-mass spectrometry-(MS)/MS technique. The study of the antimicrobial activity of these extracts was performed for eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi. RESULTS: Chromatographic analysis of hydroalcoholic extracts of both investigated Thymus species showed very similar phenolic compounds composition. In both cases, the major components are luteolin-7-O-glucoside and rosmarinic acid. About 70% ethanol extracts of T. rasitatus and T. eremita have a broad spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at concentration range of 0.0195–20 mg/ml, but differ in their potency against tested strains of microorganisms. CONCLUSION: About 70% ethanol extracts of T. rasitatus and T. eremita, endemic plants in the flora of Kazakhstan, can be considered as potential drugs with a wide spectrum of antimicrobial activity. The results of chromatographic analysis could be used for drug standardization.

Download Full-text