Chromatographic analysis, antiproliferative effect and genotoxicity of aqueous extracts of Citrus sinensis (L.) osbeck on the Allium cepa L. test system

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

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Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L.

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420130170 ◽

2014 ◽

Vol 86 (3) ◽

pp. 1131-1137 ◽

Cited By ~ 7

Author(s):

ERASMOVLANE S.B. NEVES ◽

PAULO MICHEL PINHEIRO FERREIRA ◽

LEONARDO H.G.M. LIMA ◽

ANA PAULA PERON

Keyword(s):

Cell Cycle ◽

Allium Cepa ◽

Root Meristem ◽

Test System ◽

Aqueous Extracts ◽

Phyllanthus Niruri ◽

Root Cells ◽

Allium Cepa L ◽

Chi Squared ◽

Meristematic Root

This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical analysis (p<0.05). From the results obtained it was observed that all four concentrations tested had significant antiproliferative effect on the cell cycle of this test system. We also found the presence of cellular aberrations such as colchicined metaphases, anaphasic and telophasic bridges, and micronuclei in the two exposure times for all concentrations evaluated. Therefore, under the conditions studied the concentrations of aqueous extracts of leaves of P. niruri showed to be cytotoxic and genotoxic.

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Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

Biotemas ◽

10.5007/2175-7925.2015v28n1p45 ◽

2014 ◽

Vol 28 (1) ◽

pp. 45 ◽

Cited By ~ 2

Author(s):

Layana Menezes da Silva ◽

Ana Paula Peron ◽

Francisco Ronielson da Silva Carvalho ◽

Livia Martins ◽

Iana Felício Bantim Calou ◽

...

Keyword(s):

Allium Cepa ◽

Antiproliferative Effect ◽

Hydroalcoholic Extract ◽

Meristematic Cells ◽

Allium Cepa L

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Citogenotoxicidade de extratos aquosos de Erythrina fusca Lour. sobre o ciclo celular de Allium cepa L / Citotoxity and genotoxity of aqueous extracts of Erythrina fusca Lour. about the cell cycle of Allium cepa L

Brazilian Journal of Development ◽

10.34117/bjdv7n10-308 ◽

2021 ◽

Vol 7 (10) ◽

pp. 99270-99285

Author(s):

Rosieli Barboza Bispo ◽

Elisa dos Santos Cardoso ◽

Nilo Leal Sander ◽

Joari Costa De Arruda ◽

Alex Souza Rodrigues ◽

...

Keyword(s):

Cell Cycle ◽

Allium Cepa ◽

Aqueous Extracts ◽

Allium Cepa L ◽

Ciclo Celular

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UPLC-ESI-MS/MS based identification and antioxidant, antibacterial, cytotoxic activities of aqueous extracts from storey onion (Allium cepa L. var. proliferum Regel)

Food Research International ◽

10.1016/j.foodres.2019.108969 ◽

2020 ◽

Vol 130 ◽

pp. 108969 ◽

Cited By ~ 2

Author(s):

Yanyan Zhou ◽

Cong Li ◽

Bang Feng ◽

Bang Chen ◽

Lihua Jin ◽

...

Keyword(s):

Allium Cepa ◽

Cytotoxic Activities ◽

Aqueous Extracts ◽

Esi Ms ◽

Allium Cepa L

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Investigation of cytotoxic and mutagenic effects of Malpighia glabra L. (barbados cherry) fruit pulp and vitamin C on plant and animal test systems

Food Science and Technology ◽

10.1590/s0101-20612012005000054 ◽

2012 ◽

Vol 32 (2) ◽

pp. 405-411 ◽

Cited By ~ 2

Author(s):

Elisângela Düsman ◽

Márcia Flores da Silva Ferreira ◽

Alessandra Paim Berti ◽

Rosinete Gonçalves Mariucci ◽

Mário Sérgio Mantovani ◽

...

Keyword(s):

Cell Division ◽

Vitamin C ◽

Allium Cepa ◽

Wistar Rats ◽

Chromosomal Abnormalities ◽

Bone Marrow Cells ◽

Recovery Period ◽

Test System ◽

Liquid Form ◽

Allium Cepa L

Fruits are important sources of nutrients in human diet, and Barbados Cherry (Malpighia glabra L.) is of particular interest due to its high content of antioxidants. Diets rich in fruits and vegetables protect individuals against diseases and cancer, but excessive intake of vitamins may act as pro-oxidant and generate changes in DNA. To evaluate the effect of different in natura (BAN) and frozen (BAF) Barbados Cherry pulp concentrations and synthetic vitamin C in liquid form (VC) on the chromosome level and the cell cycle division, root meristeme cells of Allium cepa L. and bone marrow cells of Wistar rats Rattus norvegicus, were used as test system. In Allium cepa L., BAN, at the highest concentration (0.4 mg.mL-1) and BAF, at the lowest concentration (0.2 mg.mL-1), inhibited cell division, and there was recovery of cell division after the recovery period in water only for BAN. In the Wistar rats, all treatments with Barbados Cherry, either acute or subchronic, were not cytotoxic or mutagenic; only the highest concentration of VC increased significantly the rate of chromosomal abnormalities. The data obtained are important to reinforce the use of Barbados Cherry fruit in the diet.

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ANTIPROLIFERATIVE AND GENOTOXIC POTENTIAL FROM EXTRACTS AND FRACTIONS OF RICHARDIA BRASILIENSIS GOMES (RUBIACEAE) BY THE ALLIUM CEPA L. TEST SYSTEM

Iheringia Série Botânica ◽

10.21826/2446-8231201772313 ◽

2017 ◽

Vol 72 (3) ◽

pp. 424-431

Author(s):

Rafaela C. Dornelles ◽

Gabriela C. Leal ◽

Ana Carla S. Decian ◽

Daniele D. dos Santos ◽

Gabriel A. Radiske ◽

...

Keyword(s):

Allium Cepa ◽

Test System ◽

Genotoxic Potential ◽

Allium Cepa L

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Differential sensitivity of Allium cepa L. and Vicia faba L. to aqueous extracts of Cascabela thevetia (L.) Lippold

South African Journal of Botany ◽

10.1016/j.sajb.2021.01.033 ◽

2021 ◽

Vol 139 ◽

pp. 67-78

Author(s):

Surochita Basu ◽

Kanika Tripura

Keyword(s):

Vicia Faba ◽

Allium Cepa ◽

Differential Sensitivity ◽

Aqueous Extracts ◽

Allium Cepa L ◽

Vicia Faba L

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CYTOGENETIC EFFECTS OF THE CRUDE SAPONINS OF Yucca elephantipesL. LEAVESONTHE Allium cepa L. ROOT TIPS MITOSIS.

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i2.980 ◽

2020 ◽

Vol 51 (2) ◽

pp. 542-549

Author(s):

Mohammed & Najem

Keyword(s):

Mitotic Index ◽

Allium Cepa ◽

Chromosomal Aberrations ◽

Test System ◽

Root Tips ◽

Cytogenetic Effects ◽

Phase Index ◽

Allium Cepa L ◽

Plant Test

This study was aimed to investigate cytogenetic effects of the crude saponinsof Yucca elephantipes leaves on mitosis. The root tips of Allium cepa L. were used as plant test system in vivo. Root tips of A. cepa were tested for four hours with four concentrations of the crude saponins (0.00, 6.25, 12.5, 25 or 50 mg/ml). This study were included some cytogenetic diagnosis included mitotic index, phase index, and chromosomal aberration. Results showed that saponins reduced the mitotic index (MI) to less than 50%, specifically when treated with 12.5,25 or 50 mg/ ml mitotic index reached 25.48, 17.98, 18.81% respectively, this reduction was considered toxic and sub lethal. Saponins arrested chromosomes at metaphase (c-metaphase) up to 100% at 6.25 mg/ml and chromosomal aberrations including micronuclei and nuclear lesion (chromatin lesion) in prophase and interphase at the concentrations 25 and 50 mg/l recorded 1.30-8.30%.

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