Differential sensitivity of Allium cepa L. and Vicia faba L. to aqueous extracts of Cascabela thevetia (L.) Lippold

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

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Aqueous Extract of (Eruca sativa Mill) as Growth Stimulant in Enhancing Growth and Yield of Faba Bean (Vicia faba L)

AL-MUKHTAR JOURNAL OF SCIENCES ◽

10.54172/mjsc.v36i1.14 ◽

2021 ◽

Vol 36 (1) ◽

pp. 57-66

Author(s):

Amal F Ehtaiwwesh ◽

Fouziyah Qarimidah

Keyword(s):

Vicia Faba ◽

Aqueous Extract ◽

Faba Bean ◽

Foliar Spray ◽

Dry Weight ◽

Growth And Yield ◽

Aqueous Extracts ◽

Eruca Sativa ◽

Vicia Faba L ◽

Growth Stimulant

A field study was conducted in the fall of 2019 to investigate the potential of Arugula (Eruca sativa Mill) aqueous extract as a growth stimulant in enhancing the growth and yield of Fa- ba Bean (Vicia faba L). The study was conducted using sandy soil at a farm in Abo Esaa town in a plot size of 3X5 m2 with a row spacing of 25cm,which based on a complete randomized design (RCD) with four replications, three treatments were carried out, including no foliar spray with E. sativa extracts (control) and foliar sprayed with 20% and 40% aqueous extracts of E. sativa. Ac- cordingly, Faba Bean (Vicia faba L) plants were foliar sprayed six times with the aqueous extracts of E. sativa at rates of 20 and 40% at vegetative and reproductive stages. The result showed that among these concentrations, the foliar spray of faba bean plants with 40% of E. sativa extracts po- tentially were increased all measured growth and yield traits. The results pointed out that plant height increased by 32%, number of branches by 73%, number of leaves by 95%, number of seeds plant by 89%, leaves, stems, pods and roots dry weight by 92%, 80%, 74%, and 89%, respectively. Thus, the study concluded that E. sativa aqueous extracts could potentially be used efficiently by crop producers as a growth enhancer for faba bean crops because of their productivity, great nutri- tive value, low cost, and environmentally friendly nature.

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EFEITO CITOTÓXICO DE EXTRATOS AQUOSOS DE ARTEMISIA ABSINTHIUM L. SOBRE O CICLO CELULAR DE ALLIUM CEPA L. / CYTOTOXIC EFFECT OF AQUEOUS EXTRACTS OF ARTEMISIA ABSINTHIUM L. ON THE CELL CYCLE OF ALLIUM CEPA L.

Brazilian Journal of Development ◽

10.34117/bjdv6n9-067 ◽

2020 ◽

Vol 6 (9) ◽

pp. 64893-64906

Author(s):

Daniele Paula Maltezo ◽

Alex Souza Rodrigues ◽

Uéliton Alves de Oliveira ◽

Larissa Lemes dos Santos ◽

Kelli Évelin Müller Zortéa ◽

...

Keyword(s):

Cell Cycle ◽

Allium Cepa ◽

Cytotoxic Effect ◽

Aqueous Extracts ◽

Artemisia Absinthium ◽

Allium Cepa L ◽

Ciclo Celular

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Chromatographic analysis, antiproliferative effect and genotoxicity of aqueous extracts of Citrus sinensis (L.) osbeck on the Allium cepa L. test system

Bioscience Journal ◽

10.14393/bj-v31n4a2015-23245 ◽

2015 ◽

Vol 31 (4) ◽

pp. 1213-1221 ◽

Cited By ~ 2

Author(s):

Marília Tedesco ◽

Andrielle Wouters Kuhn ◽

Aline Augusti Boligon ◽

Haywood Dail Laughinghouse IV ◽

Margareth Linde Athayde ◽

...

Keyword(s):

Allium Cepa ◽

Chromatographic Analysis ◽

Citrus Sinensis ◽

Test System ◽

Antiproliferative Effect ◽

Aqueous Extracts ◽

Allium Cepa L

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Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L.

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420130170 ◽

2014 ◽

Vol 86 (3) ◽

pp. 1131-1137 ◽

Cited By ~ 7

Author(s):

ERASMOVLANE S.B. NEVES ◽

PAULO MICHEL PINHEIRO FERREIRA ◽

LEONARDO H.G.M. LIMA ◽

ANA PAULA PERON

Keyword(s):

Cell Cycle ◽

Allium Cepa ◽

Root Meristem ◽

Test System ◽

Aqueous Extracts ◽

Phyllanthus Niruri ◽

Root Cells ◽

Allium Cepa L ◽

Chi Squared ◽

Meristematic Root

This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical analysis (p<0.05). From the results obtained it was observed that all four concentrations tested had significant antiproliferative effect on the cell cycle of this test system. We also found the presence of cellular aberrations such as colchicined metaphases, anaphasic and telophasic bridges, and micronuclei in the two exposure times for all concentrations evaluated. Therefore, under the conditions studied the concentrations of aqueous extracts of leaves of P. niruri showed to be cytotoxic and genotoxic.

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